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The current study demonstrates development of an eco-friendly technique for intensified synthesis of designer lipids having numerous nutraceutical benefits.Among the different properties of the hydrophobic semiconductor surfaces, self-cleaning promoted by solar illumination is probably one of the most attractive from the technological point of view. The use of sonochemistry for nanomaterials' synthesis has been recently employed for the associated shorter reaction times and efficient route for control over crystal growth and the management of the resulting material's photocatalytic properties. Moreover, the sol-gel method coupled to sonochemistry modifies the chemical environment, with reactive species such as •OH and H2O2, which yield a homogeneous synthesis. TLR2INC29 Therefore, in the following investigation, the sol-gel method was coupled to sonochemistry to synthesize a SiO2@TiO2 composite, for which the sonochemical amplitude of irradiation was varied to determine its effect on the morphology and mechanical and self-cleaning properties. SEM and AFM characterized the samples of SiO2@TiO2 composite, and while the micrographs indicate that a high ultrasonic energy results in an amorphous SiO2@TiO2 composite with a low rugosity, which was affected in the determination of the contact angle on the surface. On the other hand, FTIR analysis suggests a significant change in both SiO2-SiO and SiO2-TiO2 chemical bonds with changes in vibrations and frequency, corroborating an important influence of the sonochemical energy contribution to the hydrolysis process. Raman spectroscopy confirms the presence of an amorphous phase of silicon dioxide; however, the vibrations of TiO2 were not visible. The evaluation of hydrophobic and self-cleaning properties shows a maximum of ultrasonic energy needed to improve the contact angle and rhodamine B (RhB) removal.The harm of pesticide residues to human health via environmental pollution in agriculture has recently become a significant livelihood issue. Herein, a new strategy for smart ultra-trace analysis of phytoregulator α-naphthalene acetic acid (NAA) residues in farmland environments and agro-products via machine learning (ML) using a nanozyme flexible electrode fabricated by two-dimensional phosphorene (BP) nanohybrid with graphene-like titanium carbide MXene (Ti3C2-MXene) on the flexible substrate surface of laser-induced porous graphene (LIPG) is proposed. Highly ambient-stable BP nanohybrid with Ti3C2-MXene is prepared by ultrasonic-assisted liquid-phase exfoliation in organic solvent containing grinding black phosphorus, cuprous chloride and, Ti3C2-MXene that is obtained by selectively etching Al layers of Ti3AlC2. Nanozyme flexible electrode is fabricated by drop-coating Ti3C2-MXene/BP that is formed through electrostatic self-assembly between positively charged BP and negatively charged Ti3C2-MXene onto LIPG that is obtained by direct laser writing on commercial polyimide and patterned via a computer-aided design system as a flexible substrate. The ML model via artificial neural network algorithm for smart output of NAA is discussed. NAA is electrochemically detected in a wide linear range of 0.02-40 μM with a low limit of detection (LOD) of 1.6 nM using a portable mini-workstation. Large and rough surfaces, excellent electrochemical response, and satisfactory practicability demonstrated the feasibility and detectability of the proposed method. This will provide a portable wireless intelligent nanozyme flexible sensing platform for cost-effective, simple, fast and, ultra-trace detection of hazardous substances in the safety of environments, products, and food in agriculture.

Electrophysiological sensing of cardiomyocytes (CMs) in optogenetic preparations applies various techniques, such as patch-clamp, microelectrode array, and optical mapping. However, challenges remain in decreasing the cost, system dimensions, and operating skills required for these technologies.

This study developed a low-cost, portable impedance plethysmography (IPG)-based electrophysiological measurement of cultured CMs for optogenetic applications.

To validate the efficacy of the proposed sensor, optogenetic stimulation with different pacing cycle lengths (PCL) was performed to evaluate whether the channelrhodopsin-2 (ChR2)-expressing CM beating rhythm measured by the IPG sensor was consistent with biological responses.

The experimental results show that the CM field potential was synchronized with external optical pacing with PCLs ranging from 250ms to 1000ms. Moreover, irregular fibrillating waveforms induced by CM arrhythmia were detected after overdrive optical pacing. Through the combined evidence of the theoretical model and experimental results, this study confirmed the feasibility of long-term electrophysiological sensing for optogenetic CMs.

This study proposes an IPG-based sensor that is low-cost, portable, and requires low-operating skills to perform real-time CM field potential measurement in response to optogenetic stimulation.

This study demonstrates a new methodology for convenient electrophysiological sensing of CMs in optogenetic applications.

This study demonstrates a new methodology for convenient electrophysiological sensing of CMs in optogenetic applications.Kras and Braf are major oncogenes. The mutation of Kras codon 12 or Braf V600E can lead to ovarian carcinoma. The detection of oncogene-related DNAs and their mutations offers solution for early diagnosis of ovarian cancer. Herein, a size-tunable multi-functional DNA hexahedral-nanostructure (DHN) has been rationally designed and modified on the electrode to response to Kras and Braf DNA. The size of DHN is controlled via polyadenines (polyA). The complete self-assembly of DHN depends on the presence of both target DNAs and two assistant probes. Meanwhile, a HRP-mimicking DNAzyme forms in DHN, which catalyzes the polymerization of aniline. The produced polyaniline is utilized as the output signal through differential pulse voltammetry (DPV). The biosensor shows the linear range from 100 fM to 1 μM, with the detection limit of 48.7 fM for Kras gene; and the linear range from 100 fM to 100 nM, with the detection limit of 44.1 fM for Braf gene, respectively. Since the current response depends on both gene sequences, the high specificity of the biosensor endows it to operate in an "OR"-type logic gate to discriminate the mutation of both genes.