Danielagerskov4176

The current results suggest that autonomic nervous system activity might modulate motor-cognitive performance. This modulatory capability might be selective for plan-based approaches, hence the applied strategy to movement selection could be decisive when it comes to the vulnerability of motor-cognitive processes towards psychosocial stress.Time-domain diffuse optics (TD-DO) allows one to probe diffusive media with recognized advantages over other working domains but suffers from a poor signal-to-noise ratio (SNR) resulting from the need to build-up the histogram of single-photon arrival times with maximum count rates (CR) of few percent of the laser pulse rate to avoid the so-called "pile-up" distortion. Here we explore the feasibility of TD-DO under severe pile-up conditions with a systematic in-silico/experimental study evaluating the effects and correctability of the distortion by means of shared figures of merit. In-silico, we demonstrate that pile-up correction allows one the retrieval of homogeneous optical properties with average error  99%, while the optimal CR needed to detect localized perturbation was found to be 83%. Experiments reported here confirm these findings despite exhibiting higher accuracy errors in the retrieval of homogeneous optical properties and higher noise in the detection of localized absorption perturbations, but in line with the state-of-the-art systems. This work validates a new working regime for TD-DO, demonstrating an increase of the SNR at constant acquisition time, but also potentially leading in the future to previously unrealizable measurements of dynamic phenomena or in spatial scanning applications.The plant pathogenic bacterium Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) has become a paradigm to investigate plant-bacteria interactions due to its ability to cause disease in the model plant Arabidopsis thaliana. Pst DC3000 uses the type III secretion system to deliver type III secreted effectors (T3SEs) directly into the plant cytoplasm. Pst DC3000 T3SEs contribute to pathogenicity by suppressing plant defense responses and targeting plant's physiological processes. Although the complete repertoire of effectors encoded in the Pst DC3000 genome have been identified, the specific function for most of them remains to be elucidated. Among those effectors, the mitochondrial-localized T3E HopG1, suppresses plant defense responses and promotes the development of disease symptoms. Here, we show that HopG1 triggers necrotic cell death that enables the growth of adapted and non-adapted pathogens. We further showed that HopG1 interacts with the plant immunity-related protein AtNHR2B and that AtNHR2B attenuates HopG1- virulence functions. These results highlight the importance of HopG1 as a multi-faceted protein and uncover its interplay with AtNHR2B.Osteoarthritis (OA) is a chronic degenerative joint disease associated with age, mechanical stress, and obesity. Echinacea purpurea is a medicinal plant that shows good anti-inflammatory, antioxidant, and immunomodulatory activities. In this study, Echinacea purpurea ethanol extract nanoparticles (Nano-EE) were prepared by encapsulating Echinacea purpurea ethanol extract (EE) in chitosan-silica nanoparticles. Obesity (OB) in Sprague-Dawley (SD) rats was induced by fed 40% high-fat diet and then anterior cruciate ligament and meniscus injury were performed to induce OA. The rats got different doses of samples by oral gavage. The encapsulation efficiency and loading capacity of Nano-EE were 69.1% and 36.1%, respectively. The average size, polydispersity index (PDI), and zeta potential (ZP) of the Nano-EE were 145 ± 11 nm, 0.24 ± 0.01, - 4.57 ± 0.44 mV, respectively. Furthermore, electron microscopic images showed that the particles were spherical and were slightly agglomerated. Moreover, it showed that the leptin content, expression of MMPs, cytokines level, NF-κB level, and iNOS production were decreased whereas collagen II expression was increased after treatment. Besides, Nano-EE ameliorated the pain caused by OA and reduced the proteoglycan loss in cartilage. These results indicated that encapsulated EE (Nano-EE) can ameliorate OA with a low dosage and are more effective than unencapsulated EE.To develop and evaluate a fully automated pipeline that analyzes color fundus images in patients with tubercular serpiginous-like choroiditis (TB SLC) for prediction of paradoxical worsening (PW). In this retrospective study, patients with TB SLC with a follow-up of 9 months after initiation of anti-tubercular therapy were included. A fully automated custom-designed pipeline was developed which was initially tested using 12 baseline color fundus photographs for assessment of repeatability. After confirming reliability using Bland-Altman plots and intraclass correlation coefficient (ICC), the pipeline was deployed for all patients. The images were preprocessed to exclude the optic nerve from the fundus photo using a single-shot trainable WEKA segmentation algorithm. Fasudil cell line Two automatic thresholding algorithms were applied, and quantitative metrics were generated. These metrics were compared between PW + and PW- groups using non-parametric tests. A logistic regression model was used to predict probability of PW for assessing binary classification performance and receiver operator curves were generated to choose a sensitivity-optimized threshold. The study included 139 patients (139 eyes; 92 males and 47 females; mean age 44.8 ± 11.3 years) with TB SLC. Pilot analysis of 12 images showed an excellent ICC for measuring the mean area, intensity, and integrated pixel intensity (all ICC > 0.89). The PW + group had significantly higher mean lesion area (p = 0.0152), mean pixel intensity (p = 0.0181), and integrated pixel intensity (p  less then  0.0001) compared to the PW- group. Using a sensitivity optimized threshold cut-off for mean pixel intensity, an area under the curve of 0.87 was achieved (sensitivity 96.80% and specificity 72.09%). Automated calculation of lesion metrics such as mean pixel intensity and segmented area in TB SLC is a novel approach with good repeatability in predicting PW during the follow-up.The constitutively active androgen receptor (AR) splice variant, AR-V7, plays an important role in resistance to androgen deprivation therapy in castration resistant prostate cancer (CRPC). Studies seeking to determine whether AR-V7 is a partial mimic of the AR, or also has unique activities, and whether the AR-V7 cistrome contains unique binding sites have yielded conflicting results. One limitation in many studies has been the low level of AR variant compared to AR. Here, LNCaP and VCaP cell lines in which AR-V7 expression can be induced to match the level of AR, were used to compare the activities of AR and AR-V7. The two AR isoforms shared many targets, but overall had distinct transcriptomes. Optimal induction of novel targets sometimes required more receptor isoform than classical targets such as PSA. The isoforms displayed remarkably different cistromes with numerous differential binding sites. Some of the unique AR-V7 sites were located proximal to the transcription start sites (TSS). A de novo binding motif similar to a half ARE was identified in many AR-V7 preferential sites and, in contrast to conventional half ARE sites that bind AR-V7, FOXA1 was not enriched at these sites. This supports the concept that the AR isoforms have unique actions with the potential to serve as biomarkers or novel therapeutic targets.Freezing-thawing actions can affect the mechanical features of soil greatly, which is vital for the stability of soil slope in cold regions. Firstly, triaxial compression tests on sand samples under undrained conditions were performed to investigate the influences of freezing-thawing cycles, which shows that the freezing-thawing actions can weaken their strength and stiffness, and with the increasing freezing-thawing cycles, both the deviatoric stress and pore water pressure decrease gradually. Then, the double hardening constitutive model was revised to model the influences of freezing-thawing cycles in consideration of the influences of freezing-thawing actions, and the model was also validated by the test results. Finally, the proposed constitutive model was incorporated into a finite element code to numerically simulate the distribution of displacement and pore water pressure of sand slope subjected to freezing-thawing cycles, which shows that the freezing-thawing actions accelerate the dissipation of the pore water pressure and enlarge the displacement of the slope. The study here can provide a help in designing and construction of civil engineering in cold regions.This report is on the observation and analysis of nonlinear magnetoelectric effects (NLME) for in-plane currents perpendicularly to the hexagonal axis in single crystals and liquid phase epitaxy grown thin films of barium hexaferrite. Measurements involved tuning of ferromagnetic resonance (FMR) at 56-58 GHz in the multidomain and single domain states in the ferrite by applying a current. Data on the shift in the resonance frequency with input electric power was utilized to estimate the variations in the magnetic parameter that showed a linear dependence on the input electric power. The NLME tensor coefficients were determined form the estimated changes in the magnetization and uniaxial anisotropy field. The estimated NLME coefficients for in-plane currents are shown to be much higher than for currents flowing along the hexagonal axis. Although the frequency shift of FMR was higher for the single domain resonance, the multi-domain configuration is preferable for device applications since it eliminates the need for a large bias magnetic field. Thus, multidomain resonance with current in the basal plane is favorable for use in electrically tunable miniature, ferrite microwave signal processing devices requiring low operating power.We assessed the retinal microvascular alterations detected by optical coherence tomography angiography (OCT-A) in pediatric and juvenile craniopharyngioma (CP) patients with chiasmal compression. We included 15 eyes of 15 pediatric or juvenile CP patients and 18 eyes of 18 healthy subjects. The evaluation of vessel density from the superficial retinal capillary plexus (SRCP), the deep retinal capillary plexus, and the radial peripapillary capillary (RPC) segments was obtained by OCT-A. The association between vessel density measures and functional and structural measurements was also analyzed. There were significant reductions in the nasal sector of the SRCP (p  less then  0.0001) and all sectors of the RPC segment vessel density (nasal, temporal, and superior; p  less then  0.0001, inferior; p = 0.0015) in CP patients postoperatively compared to the healthy subjects. The peripapillary retinal nerve fiber layer (r = 0.6602, p = 0.0074) and ganglion cell-inner plexiform layer thicknesses (r = 0.7532, p = 0.0030) were associated with RPC segment vessel density. Visual acuity (r = - 0.5517, p = 0.0330) and temporal visual field sensitivity loss (r = 0.5394, p = 0.0465) showed an association with SRCP vessel density. In pediatric and juvenile patients with CP, parafoveal and peripapillary vascular changes following chiasmal compression were observed. The changes in vascular structures were closely related to structural and functional outcomes.