Cummingseliasen1676

In the present paper, we investigated the effects of rCsNOSIP on the lipopolysaccharide (LPS)-induced activated RAW264.7, a murine macrophage cell line. We found that endotoxin-free rCsNOSIP significantly promoted the levels of nitric oxide (NO) and reactive oxygen species (ROS) after pretreated with rCsNOSIP, while the level of SOD decreased. Furthermore, rCsNOSIP could also increase the level of lipid peroxidation MDA. Taken together, these results suggested that CsNOSIP was a key molecule which was involved in the production of nitric oxide (NO) and its reactive intermediates, and played an important role in oxidative stress during C. sinensis infection.This paper follows the study from 2013 focused on the molecular screening of mosquitoes as vectors of Dirofilaria spp. which provided the information on Aedes vexans as a potential vector of Dirofilaria repens in Slovakia. Current entomological and molecular research indicates that Ae. vexans can participate also in the transmission of Dirofilaria immitis within the region. Using the standard PCR method, we examined 10,500 mosquitoes (Ae. vexans, Ae. rossicus, Anopheles maculipennis s.l., Ochlerotatus sticticus, Ochlerotatus cantans, Ochlerotatus caspius, Culex pipiens/Culex torrentium, Coquillettidia richiardii), collected using CO2-baited traps at six locations in the Eastern Slovakia. Out of 105 pools, 6 pools of mosquitoes Ae. vexans were positive for D. repens DNA (minimum infective rate in Ae. vexas was 66.900, i.e. 0.8 per 1.000 mosquitoes), within which 4 were concurrently positive for D. immitis (minimum infective rate in Ae. vexans was 46.900 i.e. 0.5 per 1.000 mosquitoes).

To investigate orbital flow parameters of ophthalmic artery (OA) in patients with pseudoexfoliation glaucoma (XFG) by ultrasonography.

This case-control study included 38 patients with XFG and 32 healthy control subjects. Brepocitinib Color Doppler imaging was performed to evaluate orbital flow parameters of OA. Receiver operating characteristic analyses were performed to define the best cutoff value.

In the XFG group, decreased peak systolic velocity (p = 0.114), end diastolic velocity (p = 0.002), and increased resistivity index (RI) (p<0.001) at OA compared to healthy controls were observed. A cutoff value 0.72 for OA RI was found to have a sensitivity of 66% and specificity of 94%.

The results support the findings of increased vascular resistance in OA XFG. High sensitivity and specificity obtained for OA RI may be useful in evaluation of future therapeutic vascular agents.

The results support the findings of increased vascular resistance in OA XFG. High sensitivity and specificity obtained for OA RI may be useful in evaluation of future therapeutic vascular agents.

To describe a novel technique to release sticking haptic of a single-piece hydrophobic acrylic intraocular lens (IOL) using irrigation-aspiration (I/A) probe.

In our technique, the I/A probe is introduced into the anterior chamber on Visco mode. Using the aspiration port of the I/A probe, the sticking haptic is held at its tip and suction force is built up until occlusion is noted. Then the haptic is nudged towards the center of the IOL along its curve. After the haptic is free from optic, the suction is released.

Several techniques have been described to release the sticking haptic such as squeezing the haptic at the site where it sticks to the IOL or using Sinskey hook for releasing the adhesion. These techniques require extra manipulation of the IOL by introduction of surgical instruments. In our technique, we used the I/A probe itself for separating the sticky haptic successfully.

This technique allows separation of sticking haptic without any extra instrumentation, thus reducing intraocular maneuvering and total surgery time.

This technique allows separation of sticking haptic without any extra instrumentation, thus reducing intraocular maneuvering and total surgery time.

To report long-term experience with intravitreal anti-vascular endothelial growth factor treatment for radiation maculopathy.

From 2005-2015, 120 consecutive patients underwent intravitreal anti-VEGF therapy for radiation maculopathy. Inclusion criteria included a diagnosis of uveal melanoma treated with plaque radiotherapy and subsequent macular radiation vasculopathy (exudate, retinal hemorrhage, intraretinal microangiopathy, neovascularization, edema). Anti-VEGF therapy involved continuous injections in 4- to 12-week intervals with doses of 1.25 mg/0.05 mL, 2.0 mg/0.08 mL, 2.5 mg/0.1 mL, or 3.0 mg/0.12 mL of bevacizumab as well as 0.5 mg/0.05 mL or 2.0 mg/0.05 mL of ranibizumab. Goals were maintenance of visual acuity and normative macular anatomy. Safety and tolerability (retinal detachment, hemorrhage, infection), visual acuity, central foveal thickness on optical coherence tomography imaging, and clinical features of radiation maculopathy were analyzed.

Progressive reductions in macular edema, hemyears.

To evaluate the efficacy and outcomes of intracameral illuminator-assisted nucleofractis technique in cataract surgery.

Since June 2012, this novel technique has been performed in all cataract cases by one surgeon (approximately 300 cases of various densities). Trenching continues until the posterior plate white reflex between an endonucleus and an epinucleus is identified (enhanced depth trench). After trenching, cracking is initiated with minimal separation force, and completion of cracking is confirmed by posterior capsule reflex (one-shot crack). With followability enhanced by an elliptical phaco mode, the divided nucleus is efficiently cut into small fragments by a chisel-shaped illuminator (phaco cut).

We have not experienced any capsular bag or zonular complications, and the effective phacoemulsification time seemed to be shorter than that with the conventional technique.

This technique simplifies the complete division of the nucleus, which is the most challenging step in safe and efficient phacoemulsification.

This technique simplifies the complete division of the nucleus, which is the most challenging step in safe and efficient phacoemulsification.

To evaluate the effect of diabetes mellitus (DM) on central corneal thickness (CCT), corneal endothelial parameters (endothelial cell density [ECD], average size [AVE], hexagonality [A6], polymegathism [SD]), and subfoveal choroidal thickness (SFCT), and to determine whether these parameters depend on the duration of DM and hemoglobin A1c (HbA1c) level.

A total of 62 patients with type 2 DM and 65 healthy subjects were examined using a noncontact specular microscope, A-scan ultrasound, and spectral-domain optical coherence tomography. The study parameters included medical history, age, eye axial length, CCT, ECD, AVE, A6, SD, and SFCT. The duration of DM and HbA1c level of the 2 latest tests were noted.

The SFCT (258.0 ± 74.4 µm) and ECD (2721.8 ± 264.1 cells/mm2) were significantly lower, and the CCT (566.7 ± 35.7 µm) higher, in diabetic patients than in the control group (313.1 ± 88.5 µm, 2967.3 ± 220.6 cells/mm2, 550.0 ± 56.4 µm, respectively) (p<0.05). There were no differences in age, sex, or axial length between the diabetic and control groups (p>0.05). Diabetic retinopathy and macular edema did not influence SFCT significantly (p>0.05). No differences in endothelial parameters between groups and no correlations among HbA1c, duration of DM, and any of the examined parameters were found (p>0.05).

Diabetic patients have thicker corneas, lower ECD, and thinner subfoveal choroid than healthy subjects.

Diabetic patients have thicker corneas, lower ECD, and thinner subfoveal choroid than healthy subjects.

To evaluate the clinical outcome of surgical treatment for macular serous detachment associated with optic disc pit with pars plana vitrectomy (PPV) without laser photocoagulation on the temporal edge.

Vitrectomy was performed in 8 eyes of 8 patients (mean age 27.25 years; range 12-57 years) with unilateral macular detachment associated with optic disc pit. All patients underwent pars plana vitrectomy (cases 1, 2, 3, and 4, PPV 20 G; cases 5, 6, 7, and 8, PPV 25 G), internal limiting membrane(ILM) peeling, and SF6 20% gas tamponade (case 1 was treated with silicone oil tamponade). Endolaser on the temporal margin of the optic disk was not performed. Every patient was observed for a follow-up period of 59.25 months after surgery. Statistical analysis was carried out using Student t test paired data. link2 p Value <0.05 was considered to be significant.

Complete retinal reattachment was achieved in 7 of 8 patients. Case 8 was operated 11 months ago and he still has a small area of subretinal fluid not completely reabsorbed. Mean preoperative best-corrected visual acuity (BCVA) was 20/83 and the mean postoperative BCVA was 20/40. Mean preoperative foveal thickness was 973 μm and mean postoperative foveal thickness was 363.5 μm. link3 Case 7 developed a macular hole after treatment.

Pars plana vitrectomy, ILM peeling, and endotamponade (SF6 20% gas) without endolaser on the temporal edge of optic disc is an effective treatment. This procedure achieved successful anatomical and functional results.

Pars plana vitrectomy, ILM peeling, and endotamponade (SF6 20% gas) without endolaser on the temporal edge of optic disc is an effective treatment. This procedure achieved successful anatomical and functional results.

Few optical coherence tomography (OCT) studies have investigated outer retinal thickness including the photoreceptor layer in glaucoma. We measured changes in the macular outer retinal thicknesses using OCT in patients with glaucoma with an average follow-up of 2.9 ± 0.8 years.

A total of 39 eyes of 39 patients with primary open-angle glaucoma were analyzed. The RTVue-100 was used to measure the macular outer retinal, macular ganglion cell complex, and circumpapillary retinal nerve fiber layer thicknesses, global loss volume, and focal loss volume. Using the paired t test, baseline parameters were compared with those at the last follow-up.

The average baseline mean deviation value in the Humphrey Field Analyzer was -2.13 dB. The ganglion cell complex thickness significantly decreased over the follow-up period (baseline thickness, 79.66 ± 7.71 μm; final thickness, 76.79 ± 7.39 μm; p<0.001). There were significant differences between baseline and final visit measurements for circumpapillary retinal nerve fiber layer thickness, global loss volume, and focal loss volume (p<0.001, p<0.001, and p = 0.004, respectively). However, there was no significant change in outer retinal thickness (baseline thickness, 167.56 ± 7.26 μm; final thickness, 167.25 ± 7.93 μm; p = 0.540).

Outer retinal thickness was not altered during the follow-up period. The stability of outer retinal thickness may indicate the reliability of OCT analysis for glaucoma follow-up.

Outer retinal thickness was not altered during the follow-up period. The stability of outer retinal thickness may indicate the reliability of OCT analysis for glaucoma follow-up.

To compare phacoemulsification parameters at different high-altitude regions as well as between peristaltic and Venturi-based machines.

In this prospective, nonrandomized clinical study, 160 eyes of 160 patients with senile cataract underwent phacoemulsification using either peristaltic or Venturi system at a high-altitude Himalayan region (>10,000 feet). Patients (n = 200, including 100 each with either peristaltic or Venturi system) operated at mean altitude of 1115 feet (Delhi) were included as controls (group 1). At Leh (11,203 feet), 110 patients were operated with peristaltic (62) or Venturi (48) system (group 2), whereas 50 patients (group 3) (peristaltic = 37; Venturi = 13) were operated either with peristaltic (37) or Venturi (13) system at Tangtse (14,106 feet). Intraoperative parameters-i.e., bottle height (BH), vacuum (V), and flow rate (FR)-were compared for different phacoemulsification steps-i.e., central chopping (CC), segment removal (SR), epinucleus removal (ER), and cortex removal (CR)-between all groups and between peristaltic and Venturi pump-based machines in each group.