Connollyniebuhr0548

The COVID-19 pandemic forced a nationwide lockdown in India for months when close to 1.3 billion people were confined to their homes. MitoQ cell line An abrupt halt in the majority of the urban activities reduced the generation of anthropogenic heat which often exacerbates the Urban Heat Island (UHI) effect in the urban pockets of the country. We studied the lockdown impact on seven highly populated and polluted mega urban agglomerations across India, namely Delhi, Ahmedabad, Hyderabad, Kolkata, Mumbai, Bengaluru and Chennai, using near-anniversary Landsat 8 data. The results revealed that the lockdowns have improved the air quality and reduced the Land Surface Temperature (LST) and hence the UHI effect over these cities. Each of the cities experienced an improved Air Quality Index (AQI) ranging from 18 to 151 units except Chennai (with a marginal 8 units increase in AQI), a decrease in mean LST in the range of 0.27 °C to 7.06 °C except Kolkata which showed an increment by ∼4 °C, and a reduction in daily averaged air temperature ranging from 0.3 °C to 10.88 °C except Hyderabad which witnessed an increase of 0.09 °C during the lockdown (April 2020) compared to the previous years (April 2019 and 2018). Delhi exhibited the maximum positive impact of the lockdown in all aspects with two-fold improved air quality, and Ahmedabad showed the least improvement. In addition to the variations in regional land use and land cover and proportion of essential industries that remained operational throughout the lockdown, the geographic location, topography, local meteorology and climate were some of the other factors also responsible for either aiding or overcompensating the large scale LST variabilities observed in these cities. These results hint at an unprecedented opportunity to evaluate the effectiveness of periodic planned lockdowns as a possible mitigating measure to reduce LST spikes and degraded air quality in urban areas in the future.Human 2-arachidonoylglycerol (2-AG) is an agonist of endocannabinoid system and acts as an important modulator of many physiological processes such as emotional state and pain sensation. Identification and quantification of 2-AG is vital for medical and pathological processes. There are no reports on the measurement of 2-AG in human biofluids using modern methods such as biosensors. This study reports an ultra-sensitive and selective immunosensor to determine endocannabinoids 2-AG in human plasma samples. In this study, gold nano-flowers (AuNFs) were synthesized and conjugated with a specific biotinylated antibody of 2-AG. Bioconjugated composite (bioreceptor with AuNFs) was immobilized on the surface of a gold electrode and used for the monitoring of the antigen (target molecules) based on the immunoreaction process. Moreover, a constructed interface was characterized by field-emission scanning electron microscopy (FE-SEM), dynamic light scattering (DLS), transmission electron microscopy (TEM) and zeta potential methods. Using the proposed immuno-platform, 2-AG was determined in two dynamic ranges of 0.00024-0.0078 ng L-1 and 2-16 ng L-1 with a lower limit of quantitation (LLOQ) of 0.00024 ng L-1. These results suggest that our immunosensor might be appropriate for an early diagnosis of 2-AG towards the screening of immunomodulatory activity and neuroprotection.The description of forces across confined complex fluids still holds many challenges due to the possible overlap of different contributions. Here, an attempt is made to untangle the interaction between charged surfaces across nanoparticle suspensions. Interaction forces are measured using colloidal-probe atomic force microscopy. The experimental force profiles are considered as a superposition of double layer and structural forces. In order to independently describe the decay of the double layer force, the ionic strength of the suspension is determined by electrolytic conductivity measurements. Jellium approximation is used to define the impact of the fluid on screening the surface potential. There, the nanoparticles are considered homogeneously distributed across the fluid and screening is only carried out via the particles counterions and added salt. The structural force follows a damped oscillatory profile due to the layer-wise expulsion of the nanoparticles upon approach of both surfaces. The description of the oscillatory structural force is extended by a depletion layer next to the confining surfaces, with no nanoparticles present. The thickness of the depletion layer is related to the electrostatic repulsion of the charged nanoparticles from the like-charged surfaces. The results show that the total force profile is a superposition of independent force contributions without any mutual effects. Using this rather simple model describes the complete experimentally determined interaction force profiles very well from surface separations of a few hundred nanometres down to the surfaces being almost in contact.Bovine milk-derived exosomes have recently emerged as a promising nano-vehicle for the encapsulation and delivery of macromolecular biotherapeutics. Here we engineer high purity bovine milk exosomes (mExo) with modular surface tunability for oral delivery of small interfering RNA (siRNA). We utilize a low-cost enrichment method combining casein chelation with differential ultracentrifugation followed by size exclusion chromatography, yielding mExo of high concentration and purity. Using in vitro models, we demonstrate that negatively charged hydrophobic mExos can penetrate multiple biological barriers to oral drug delivery. A hydrophilic polyethylene glycol (PEG) coating was introduced on the mExo surface via passive, stable hydrophobic insertion of a conjugated lipid tail, which significantly reduced mExo degradation in acidic gastric environment and enhanced their permeability through mucin by over 3× compared to unmodified mExo. Both mExo and PEG-mExo exhibited high uptake by intestinal epithelial cells and mediated functional intracellular delivery of siRNA, thereby suppressing the expression of the target green fluorescence protein (GFP) gene by up to 70%. We also show that cationic chemical transfection is significantly more efficient in loading siRNA into mExo than electroporation. The simplicity of isolating high purity mExo in high concentrations and equipping them with tunable surface properties, demonstrated here, paves way for the development of mExo as an effective, scalable platform technology for oral drug delivery of siRNA.