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Surface tension was measured by solving the Young-Laplace equation for pendant drop shape hanging on a vertical needle. An anticipated outcome of this study would be the development of a novel automated classifier based upon the rheological attributes of blood. #link# This automated classifier would have potential application in evaluating the health status of a population at regional and global levels. Results The proposed system was used to measure the physical properties of various samples like normal, tuberculous and anemic blood samples. The results showed that the physical properties of these samples were different as compared to normal blood samples. The major advantage of this system was low-cost, as well as its simplicity and portability. Tolinapant In this work, we proposed making a case for the validation of a low-cost version of a microfluidic system capable of scanning large populations for a variety of diseases as per the WHO mandate of "One Health".The COVID-19 pandemic may exacerbate factors influencing abusive workplace behaviors in general such as psychological health, economic and social inequities. This is true in academic and research environments where we can expect to see an increase in the incidence of academic bullying. Research and experience shows that academic bullying will have significant and enduring negative effects on scientific integrity and academic health. In this perspective piece we will explore the potential facilitative influence of COVID-19 and specifically responses to it, on bullying behaviors in academic and research environments.COVID-19 pandemic has profoundly affected the lives of humans worldwide. We no longer experience the same quality of life and need to come up with effective solutions to combat the clinical implications. The vast knowledge about the pathways that regulate the virus entry and molecular signaling of the pathogenesis of coronavirus are the key factor for the development of de novo diagnostic/therapeutic strategies. Meanwhile, the emergence of nanotechnology, could offer enormous help in the battle against coronavirus. link2 In this editorial, the role of molecular elements in the pathobiology of the disease and the significance of nanoscaled pharmaceuticals is highlighted.While nucleic acid and protein analysis approaches continue to see significant breakthroughs, analytical strategies for glycan determination have by comparison seen slower technological advances. Here we provide a strategy for glycan probe development using an engineered lectin fusion that can be incorporated into various common pathology lab assay formats including Western blot and agglutination assays. In this proof of concept, we use the natural lectin, Pseudomonas fluorescens agglutinin (PFA), capable of binding core Man alpha(1-3)-Man alpha(1-6)-Man units, where this lectin has previously been shown to bind to the glycans presented by the gp120 coat protein of (HIV) Human Immunodeficiency Virus. In our strategy, we engineered the lectin to possess a fusion of the biotin mimetic tag equence of amino acids V-S-H-P-Q-A-P-F. With the glycan receptive PFA directly linked to the biotin mimic, we could facilitate a probe for various standard clinical assay formats by virtue of coupling to streptavidin-HRP (horseradish peroxidase) or streptavidin beads for Western blot and agglutination assays respectively. We found the PFA fusion retained low nanomolar affinity for gp120 by ELISA (Enzyme Linked Immunosorbent Assay) and microscale thermophoresis. This probe engineering strategy proved effective in the relevant assay formats that may now allow detection for the presence of glycans containing the core Man alpha(1-3)-Man alpha(1-6)-Man units recognized by PFA.Vasosensory reflex responses are elicited by instillation of nociceptive agents in a segment of peripheral blood vessel. A novel method for the stimulation of perivascular afferents was designed by retrograde cannulation of femoral artery, using a 24G, double ported polyethylene cannula. The vertical port of which was used to inject the algogen into the artery and horizontal port to measure the BP continuously, as this port was connected to the pressure transducer. Previously, separate carotid artery cannulation was used for the BP recording. But our experimental design excluded the need for carotid artery cannulation that might compromise the circulation to the CNS centers mediating cardiorespiratory reflex responses. link3 After cannulation, the proximal end of femoral artery became an end artery and the drugs were instilled retrogradely. The volume of chemicals was kept minimal (100 µl) and the ipsilateral femoral vein was also ligated. These measures made sure that the instilled drug remained in a local segment of femoral artery and did not spill out to the systemic circulation. Further, there was no increase in the water content of ipsilateral paw as compared to the contralateral paw. This finding also substantiates our proposition regarding minimal systemic spillage.•The femoral artery is cannulated by a double ported cannula.•This cannula helps instillation of algogen and BP measurement simultaneously.•Retrograde instillation helps to deposit the algogen in a local segment of femoral artery.Hybrid methodologies have become popular in many fields of research as they allow researchers to explore various methods, understand their strengths and weaknesses and combine them into new frameworks. Thus, the combination of different methods into a hybrid methodology allows to overcome the shortcomings of each singular method. This paper presents the methodology for two hybrid methods that can be used for time series forecasting. The first combines singular spectrum analysis with linear recurrent formula (SSA-LRF) and neural networks (NN), while the second combines the SSA-LRF and weighted fuzzy time series (WFTS). Some of the highlights of these proposed methodologies are•The two hybrid methods proposed here are applicable to load data series and other time series data.•The two hybrid methods handle the deterministic and the nonlinear stochastic pattern in the data.•The two hybrid methods show a significant improvement to the single methods used separately and to other hybrid methods.This article introduces a methodology that aims to develop a detailed framework for measuring land-use changes in an area affected by several impact factors, especially by a new project which is economically and spatially critical in an urban area. The basis of the methodology lays on the Sieve approach that analyzes changes by categorizing different factors in an urban settlement or even on vacant lands. It is a multi-factorial approach that evaluates and simulates land-use changes in an urban area with a newly-emerged impact factor (Airport in this study). Factors such as proximity to Transportation nodes, Commercial areas, Industrial areas, Health centers, Educational areas, and many others that define investors, users, and planners' tendencies are all critical parameters that can be used as inputs of this quantitative approach. Function Impact Analysis Oriented Land-Use Assessment Model (APELUM), developed in this study, is a new method useful for measuring land cover changes in an urban area even before it takes place. It can be used as a method for analyzing different scenarios to determine the strengths and weaknesses of each model. Two different scenarios, supported by two different spatial models, are evaluated "Demands and trends of the users and investors in the urban area" that simulates rapid and unplanned development and "The planning decisions and user trends" to simulate strategic development. The methodology uses AutoCAD and ArcGIS tools for producing maps and making different measurements.•This quantitative model and similar ones are useful tools for measuring the range of land-use changes after or before the implementation of a new project in an urban area.•It generates quantitative estimations based on spatial data in the planning process of cities.•It can be useful to develop simulation models that make correlations between different features of measured tendencies.Runoff monitoring experiments are often conducted in remote sites. Sample collection and dispatch for analysis are often delayed due to sites' remoteness and limited local laboratory facilities. The standard method of dissolved organic carbon (DOC) and total organic carbon (TOC) analysis in water samples requires storage of the samples at 4 °C after collection and analysis with a minimum of delay. However, there is no freezing storage time limit specified to avoid degradation. To overcome the limitations of this approach, we investigated the method of storage, that is refrigeration (4 °C) versus freezing (-18 °C), and the effect of storage time on DOC and TOC fractions in runoff water. Storage of samples at 4 °C for more than a week resulted in a decline of TOC and DOC concentrations in runoff water.•Freezing unfiltered water samples immediately after collection minimized TOC losses during storage, however, it may lead to variable DOC results.•Filtering a subsample of runoff or irrigation water immediately after collection using a 0.45 µm filter and freezing both the filtered and unfiltered samples until analysis of DOC and TOC, respectively, can minimize losses during storage.The presence of pharmaceutically active compounds (PhACs) in aquatic biota has been received much less attention than their presence in surface or waste water, and it was not until the mid-2000s, this gap started to be addressed. Here, we present SQUEEZe (Solid-liQuid Ultrasound Extraction with QuE Z-Sep/C18 as dispersive clean-up) a fast method for analysis of the trace 47 PhACs in fish muscle. Compared to our previously reported method [1], it offers alternatives with improvements in recoveries, number of analytes, sample volume and solvent used. The key aspects of this method are•The ultrasound extraction was performed with acetonitrile/isopropanol 0.1% V/V formic acid. A clean-up step using QuE Z-Sep/C18 sorbents was employed to reduce lipid content of the extracts and further matrix effects in the detection of the analytes.•A HPLC separation with a Kinetex EVO C18 packed column in 11 min was optimized. MS and MS/MS data were collected using SWATH acquisition on the SCIEX X500R QTOF in (+)-ESI mode.•The method validated at 3 different concentrations levels 5, 25 and 50 ng/g fish. It presented good intraday/interday reproducibility and absolute recoveries ≥ 60% for majority of analytes in composite homogenate muscle matrix of Squalius cephalus.•10 out 47 compounds were detected in fish samples.We established a laboratory propagation method of Cryptocaryon irritans, a parasitic ciliate of marine fishes, with black molly Poecilia sp. as host fish, using small plastic aquaria. One cycle of the propagation usually takes one week. With this method, 1500-3000 protomonts are obtained from five challenged mollies every week, from which more than 100,000-200,000 theronts are obtained. Using this method, an isolate of C. irritans has been successfully maintained more than three years. This propagation method reduces labor for maintaining and propagating the parasite and will much contribute to researches on cryptocaryoniasis.•The method is a laboratory propagation technique of Cryptocaryon irritans.•Using small plastic aquaria and black molly as a host, the parasites can be stably propagated and maintained.•An isolate of C. irritans has been successfully maintained more than three years.