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These results provided information about the mechanisms involved in drought response of common bean response that could be useful for enhancing the drought tolerance of this important crop legume.Various traffic-sensing technologies have been employed to facilitate traffic control. Due to certain factors, e.g., malfunctioning devices and artificial mistakes, missing values typically occur in the Intelligent Transportation System (ITS) sensing datasets, resulting in a decrease in the data quality. In this study, an integrated imputation algorithm based on fuzzy C-means (FCM) and the genetic algorithm (GA) is proposed to improve the accuracy of the estimated values. The GA is applied to optimize the parameter of the membership degree and the number of cluster centroids in the FCM model. An experimental test of the taxi global positioning system (GPS) data in Manhattan, New York City, is employed to demonstrate the effectiveness of the integrated imputation approach. Three evaluation criteria, the root mean squared error (RMSE), correlation coefficient (R), and relative accuracy (RA), are used to verify the experimental results. Under the ±5% and ±10% thresholds, the average RAs obtained by the integrated imputation method are 0.576 and 0.785, which remain the highest among different methods, indicating that the integrated imputation method outperforms the history imputation method and the conventional FCM method. On the other hand, the clustering imputation performance with the Euclidean distance is better than that with the Manhattan distance. Thus, our proposed integrated imputation method can be employed to estimate the missing values in the daily traffic management.Circadian rhythm is an endogenous, self-sustainable oscillation that participates in regulating organisms' physiological activities. Key to this oscillation is a negative feedback by the main clock components Periods and Cryptochromes that repress the transcriptional activity of BMAL1/CLOCK (defined in the Abbreviations) complexes. In addition, a novel repressor, CHRONO, has been identified recently, but details of CHRONO's function during repressing the circadian cycle remain unclear. Here we report that a domain of CHRONO mainly composed of α-helixes is critical to repression through the exploitation of protein-protein interactions according to luciferase reporter assays, co-immunoprecipitation, immunofluorescence, genome editing, and structural information analysis via circular dichroism spectroscopy. This repression is fulfilled by interactions between CHRONO and a region on the C-terminus of BMAL1 where Cryptochrome and CBP (defined in the Abbreviations) bind. Our resultsindicate that CHRONO and PER differentially function as BMAL1/CLOCK-dependent repressors. Besides, the N-terminus of CHRONO is important for its nuclear localization. click here We further develop a repression model of how PER, CRY, and CHRONO proteins associate with BMAL1, respectively.Pesticides are among the most important contaminants in food, leading to important global health problems. While conventional techniques such as high-performance liquid chromatography (HPLC) and mass spectrometry (MS) have traditionally been utilized for the detection of such food contaminants, they are relatively expensive, time-consuming and labor intensive, limiting their use for point-of-care (POC) applications. Electrochemical (bio)sensors are emerging devices meeting such expectations, since they represent reliable, simple, cheap, portable, selective and easy to use analytical tools that can be used outside the laboratories by non-specialized personnel. Screen-printed electrodes (SPEs) stand out from the variety of transducers used in electrochemical (bio)sensing because of their small size, high integration, low cost and ability to measure in few microliters of sample. In this context, in this review article, we summarize and discuss about the use of SPEs as analytical tools in the development of (bio)sensors for pesticides of interest for food control. Finally, aspects related to the analytical performance of the developed (bio)sensors together with prospects for future improvements are discussed.GM1-gangliosidosis is caused by a reduced activity of β-galactosidase (Glb1), resulting in intralysosomal accumulations of GM1. The aim of this study was to reveal the pathogenic mechanisms of GM1-gangliosidosis in a new Glb1 knockout mouse model. Glb1-/- mice were analyzed clinically, histologically, immunohistochemically, electrophysiologically and biochemically. Morphological lesions in the central nervous system were already observed in two-month-old mice, whereas functional deficits, including ataxia and tremor, did not start before 3.5-months of age. This was most likely due to a reduced membrane resistance as a compensatory mechanism. Swollen neurons exhibited intralysosomal storage of lipids extending into axons and amyloid precursor protein positive spheroids. Additionally, axons showed a higher kinesin and lower dynein immunoreactivity compared to wildtype controls. Glb1-/- mice also demonstrated loss of phosphorylated neurofilament positive axons and a mild increase in non-phosphorylated neurofilament positive axons. Moreover, marked astrogliosis and microgliosis were found, but no demyelination. In addition to the main storage material GM1, GA1, sphingomyelin, phosphatidylcholine and phosphatidylserine were elevated in the brain. In summary, the current Glb1-/- mice exhibit a so far undescribed axonopathy and a reduced membrane resistance to compensate the functional effects of structural changes. They can be used for detailed examinations of axon-glial interactions and therapy trials of lysosomal storage diseases.In this article, we report the preparation of lignin-derived carbon nanosheet (L-CNS) by direct thermal treatment of lignin without activation operation and the functions of the L-CNS as an adsorbent for rhodamine dye. The L-CNSs are fabricated by freeze-drying (FD) methods of lignin followed by high-temperature carbonization. It is found that lower frozen temperature in FD or lower concentration of lignin aqueous solution renders L-CNSs' more porous morphology and higher specific surface area (SSA), allowing a promising application of the L-CNSs as an efficient adsorbent for organic pollutants. In particular, the alkaline hydroxide catalyst helps to increase the SSA of carbon products, leading to a further improved adsorption capacity. On the other hand, p-toluenesulfonic acid (TsOH) catalyzed pyrolysis, which dramatically increased the L-CNS product yield, and provided a high-yield approach for the production of pollutant absorbent.