Clementsmacmillan3591

Phototheranostics has attracted great interest in cancer therapy. Small-molecule self-reporting photosensitizers, one kind of idea agent in phototheranostics, enables simultaneous photodynamic therapy (PDT) and feedback of therapeutic efficacy. However, previous such photosensitizers exclusively employed the change of single emission to monitor cell death, which can be disturbed by variations in photosensitizer concentration and the excitation intensity. Herein, we report a unique self-reporting photosensitizer TPA-3PyA+ constructed from a twisted triphenylamine unit (TPA), three benzene ring units and three cyanovinyl-pyridinium units (PyA) for PDT and its real-time monitoring in a dynamic dual-color mode. TPA-3PyA+ possesses a rotatable electron donor-π bridge-electron acceptor framework and exhibits high singlet oxygen quantum yield (124%) and a twisted intramolecular charge transfer (TICT) effect. TPA-3PyA+ not only enables effective staining of cancer cells with dual-color fluorescence due to the TICT effect but also shows excellent PDT performance. The simultaneous change in emission color, intensity and intracellular location of TPA-3PyA+ during cell death allows it to self-report cell death. Moreover, the change of dual-emission color allows distinguishing living and dead cells and effectively avoids interference in previous single-emission self-reporting photosensitizers. This work highlights the great potential of a self-reporting photosensitizer with dual-color emissions for efficient feedback of theranostics.Different weight percentages of NiCo2O4-rGO nanocomposites were prepared via a facile hydrothermal method. The prepared nanocomposites were structurally and morphologically characterized by X-ray diffraction, Raman spectroscopy, and electron microscopy. The structural studies show the formation of rGO-NiCo2O4 nanocomposites by embedment of porous NiCo2O4 rods on rGO sheets. The effect of the NiCo2O4 content on photochemical water oxidation was investigated. It revealed that the catalysts NiCo2O4-rGO with 1  26 ratio (NCO26) and 1  13 ratio (NCO13) are efficient in generating oxygen under light illumination. It proves that NCO26 works far more effectively as a photocatalyst compared to NCO13. Methanol electro-oxidation of the NCO26 nanocomposite shows a current density of 24 mA cm-2 at a potential of 0.45 V in cyclic voltammetry and maintains the current for 3600 s at 0.45 V in chronoamperometry. An onset potential of 0.344 V was observed for 0.5 M methanol oxidation. The specific capacitance values were found to be 354.75 F g-1 and 375.32 F g-1 at 1 mV s-1 and 1 A g-1, respectively, for NCO26 in supercapacitor studies. The charge stored via capacitive and diffusion-controlled processes was determined using Power's law and Trasatti plot. An asymmetric supercapacitor device shows a specific capacitance of 122.2 F g-1 at a current density of 1 A g-1 and exhibits a retention of 74.3% after 5000 cycles. An energy density of 67.89 W h kg-1 and a power density of 1 kW kg-1 at a current density of 1 A g-1 are observed.We designed and synthesized a fatty aldehyde surrogate containing a formyl thioester group, which can be reduced by fatty aldehyde reductase (FALR) with stoichiometric formaldehyde generation. It can be rapidly visualized and quantified using the Purpald assay. We demonstrated its successful application in the high throughput screening of FALR engineering.Anthocyanins have anti-inflammatory, anticarcinogenic and antioxidant properties and anti-aging effects as well as potential application as pigments. The metabolism of anthocyanins in fermented food has attracted increasing attention. However, the effect of lactic acid bacteria (LAB) fermentation on its anti-aging activity remains mostly unknown. The current study aimed to investigate the compositions, antioxidant activities and anti-aging effect of fermented purple sweet potato anthocyanins (FSPA) on aging Caenorhabditis elegans compared to raw purple sweet potato anthocyanins (PSPA). Results showed that anthocyanins were degraded into more bioavailable phenolic acids by Weissella confusa fermentation. PSPA and FSPA can extend the lifespan of C. elegans by 26.7% and 37.5%, respectively, through improving the activity of antioxidant enzymes as well as decreasing MDA content, ROS levels and lipofuscin accumulation. Pretreatment of the worms with PSPA and FSPA induced their potential to resist to thermal tolerance and oxidative stress, and FSPA exerted a higher anti-stress effect than PSPA. Moreover, FSPA supplementation upregulated the mRNA expressions of genes daf-16, hsp-16.2, sir-2.1, skn-1 and sod-3 and downregulated the expression of daf-2 in the nematodes, whereas PSPA only induced the increase in the expressions of sir-2.1, skn-1 and sod-3. Overall, FSPA can improve stress resistance and extend the lifespan of C. elegans by both insulin/IGF-1 signaling pathway and dietary restriction pathway, providing a theoretical basis for the application of PSPA in fermented food as functional pigments.The hexagonal-phase (β) of NaREF4 upconversion nanocrystals (RE = rare earth elements) has been widely employed because of the outstanding luminescence performance, yet less is known about the essence of this superior property. The current understanding of this issue is raised from the advantage of weak electron-vibration interactions in fluoride systems, while the interpretability of this statement is controversial and contradictory results are commonly reported. One feasible way to solve this puzzle is from the aspect of "structure-property" relationship, yet even after decades of investigation, the structural details of β-NaREF4 are still under debate. Herein, the reported results relevant to this topic are reviewed, and the conflicting viewpoints are summarized. The similarities and differences between different lattice templates are assessed, and the reasons underlying the divergence are analysed. Based on these discussions, it is realized that the crystal structure of β-NaREF4 should be more reliably depicted as one flexible lattice framework with complex characteristics, and the structural disorder induced by atom displacements in the lattice is probably the key to supporting the superior luminescence properties of β-NaREF4 nanocrystals.Failure of sulfate-reducing bacteria (SRB)-mediated treatment of acid mine drainage (AMD) in cold regions due to inhibition of bacteria by acidic pH and low temperature can be overcome by enriching psychrophilic and acidophilic microbial consortia from local metal-rich sediments. In this study, we enriched microbial consortia from Arctic mine sediments at varying pH (3-7) and temperatures (15-37 °C) under anaerobic conditions with repeated sub-culturing in three successive stages, and analyzed the microbial community using 16S rRNA gene sequencing. The enriched SRB genera resulted in high sulfate reduction (85-88%), and significant metal removal (49-99.9%) during the initial stages (stage 1 and 2). Subsequently, sub-culturing the inoculum at pH 3-4.5 resulted in lower sulfate reduction (9-34%) due to the inhibition of SRB by accumulated acetic acid (0.3-9 mM). The microbial metabolic interactions for successful sulfate and metal removal involved initial glycerol co-fermentation to acetic acid at acidic pH (by Desulfosporosinus, Desulfotomaculum, Desulfurospora, and fermentative bacteria including Cellulomonas and Anaerovorax), followed by acetic acid oxidation to CO2 and H2 (by Desulfitobacterium) at neutral pH, and subsequent H2 utilization (by Desulfosporosinus). The results, including the structural and functional properties of enriched microbial consortia, can inform the development of effective biological treatment strategies for AMD in cold regions.Atomic layer deposition (ALD) is a thin film deposition technique based on self-saturated reactions between a precursor and reactant vacuum conditions. A typical ALD reaction consists of the first half-reaction of the precursor and the second half-reaction of the counter reactant, in which the terminal groups on the surface change after each half-reaction. GS-0976 price In this study, the effects of counter reactants on the surface termination and growth characteristics of ALD HfO2 thin films formed on Si substrates using tetrakis(dimethylamino)-hafnium (TDMAH) as a precursor were investigated. Two counter reactants, H2O and O3, were individually employed, as well as in combination with consecutive exposure by H2O-O3 and O3-H2O. The film growth behaviors and properties differed when the sequence of exposure of the substrate to the reactants was varied. Based on X-ray photoelectron spectroscopy (XPS) and density functional theory (DFT) simulation, the changes are attributed to the effects of the surface terminations formed from different counter reactant combinations. The knowledge from this work could provide insight for precisely tuning the growth and properties of ALD films.Phosphor is an important part of the new generation of light-emitting diodes (LEDs), which requires high luminous intensity and high-temperature resistance. In this study, a series of excellent (Ba1-x-yCax)AlSi5O2N7yEu2+ phosphors was developed, which were synthesized by a high-temperature solid-state reaction in a reducing atmosphere. In addition, the crystallinity and luminescence intensity of the samples could be enhanced by some amount of Ca2+ substitution. The luminescence intensity was the highest when the Eu2+ concentration reached 0.06. Furthermore, the thermal stability of the luminescence was studied in detail. The results were satisfactory, showing that the luminescence intensity of the (Ba1-xCax)AlSi5O2N7Eu2+ phosphors exhibited unique negative-thermal-quenching characteristics both at high (273-473 K) and low (4-273 K) temperatures. And the phosphor combined with UV LED chip and red phosphor Sr2Si5N8Eu2+ can achieve a CRI of 90.4 in white LED application which indicated the (Ba1-xCax)AlSi5O2N7Eu2+ phosphor has potential in LED applications.Lipid droplets (LDs) are dynamic multifunctional organelles that participate in the regulation of many metabolic processes, visualization of which is necessary for biological research. In this work, a series of two-photon responsive fluorescent probes (C-H, C-Br, and C-I) based on carbazole units were designed and synthesized. Thereinto, an iodine-modified carbazole derivative C-I exhibited an exciting lipid droplet targeting ability due to its excellent lipophilicity. Meanwhile, benefiting from its larger Stokes shift and two-photon absorption cross-section, C-I was employed for two-photon confocal laser scanning microscopy (CLSM) and stimulated emission depletion (STED) microscopy imaging to observe LDs more accurately. In addition, given the heavy atom effect, C-I can effectively generate reactive oxygen species (ROS) leading to cancer cell apoptosis under near-infrared light irradiation. Notably, we explained the process of cell apoptosis through in vitro simulation experiments. This study provides a promising platform for visualization of lipid droplets.The global COVID-19 pandemic starting at 2020 induced by the severe acute respiratory syndrome coronavirus 2 virus (SARS-CoV-2) has revealed a very pressing need for rapid, affordable and effective diagnosis for epidemic management and control. Although several commercialized analytical methods (e.g., reverse transcription polymerase chain reaction and enzyme linked immunosorbent assay) have been developed for detecting SARS-CoV-2, they are expensive and time-consuming. Most recently, low-cost molecularly imprinted polymer (MIP)-based sensors have received attention. In this study, by introducing gold/graphene (Au/Gr) nanohybrids to modify a screen-printed carbon electrode (SPCE) and using arginine as the functional monomer, a simple and highly sensitive MIP sensor was proposed to detect SARS-CoV-2 nucleocapsid protein (ncovNP). By optimizing various influencing factors, the proposed MIP sensor shows wide linear range and low detection limit for ncovNP owing to excellent electrical property and large surface of Au/Gr and specific recognition ability of MIP, revealing important potential application for the effective early diagnosis of COVID-19.