Choijama5846
This study aimed to develop a loop-mediated isothermal amplification (LAMP) assay for the rapid detection of tick-borne relapsing fever in resource-limited areas.
A set of six primers were designed based on the conserved regions of the Glycerophosphodiester phosphodiesterase (
) gene of
species. For sensitivity assay, serial dilutions of a recombinant plasmid containing a 219bp sequence of the glpQ were prepared and used as the template DNA. The LAMP reactions containing the six primers and the reagents required for amplification were incubated at 60-65 °C for 60min in a Loopamp real-time turbidimeter. For the specificity test, DNA from 14 other bacteria were included in the assays, and double-distilled water was used as the negative control. Also, DNA from dried blood spots (DBSs) of spirochetemic mice, and blood samples from relapsing fever-suspected patients were examined by the LAMP along a
-specific nested PCR that targets the
IGS region.
The LAMP detected as low as 90
copies in reactions. The primers reacted with DNA from DBS of spirochetemic mice showing spirochete concentrations of ≤ one per a 1000X microscopic field. In clinical samples, the LAMP assay showed a higher sensitivity compared to nested-PCR. The LAMP specificity was 100%, as the primers did not react with other bacteria DNA.
The high sensitivity and specificity of the test, along with the simplicity of the DNA extraction procedure, make the LAMP a reliable and adaptable tool for the diagnosis of tick-borne relapsing fever in rural endemic areas.
The high sensitivity and specificity of the test, along with the simplicity of the DNA extraction procedure, make the LAMP a reliable and adaptable tool for the diagnosis of tick-borne relapsing fever in rural endemic areas.
Mediterranean form of visceral leishmaniasis (VL) is endemic among some provinces of Iran. The present study was designed to determine the prevalence of canine visceral leishmaniasis (CVL) in the owned dogs of the rural areas of Alborz Province near Tehran as the capital of Iran.
This study conducted on 303 owned dogs that selected using a stratified random sampling method. The direct agglutination test (DAT) was used to determine the frequency of Vl. The spleen biopsy was taken from the serology-positive dogs for the confirmation of CVL in the suspected dogs. Nested PCR and sequencing methods were used to determine the type of
species in the dogs which were parasitological positive.
Overall, the DAT results of 9 dogs (2.97%, CI 1.57-5.55) showed anti
antibodies at titers ≥ 1320 indicating VL infection. One dog (0.33%, CI 95% 0.06-1.85) showed clinical signs and symptoms of VL. There was a significant correlation between the positive cases of CVL and rural area (p< 0.001). The
was observed in the impression smears that were prepared from spleen biopsy of five the studied dogs.
were confirmed in all them using nested-PCR assay. The sequence analysis of all five isolates was 95% similar to
.
This study shows that domestic cycle of
has been established in rural areas of Alborz province where located near Tehran as capital city of Iran. It is necessary to increase the awareness and monitoring of the disease periodically.
This study shows that domestic cycle of L. infantum has been established in rural areas of Alborz province where located near Tehran as capital city of Iran. It is necessary to increase the awareness and monitoring of the disease periodically.
The common bed bug, a nocturnal bloodsucking insect, is known as a human parasite and public health problem in the world. The prevalence and geographical dispersion of bed bug in Ahvaz City, southwest of Iran was measured.
Spatial distribution of
was determined by surveying 520 houses in 62 areas of Ahvaz City in 2017. GDC-0980 inhibitor Some information like as infested points, the concern level of the residents and allergic reaction to the insect bite were registered in a form using the secondhand instrument.
According to the spatial distribution map, of 62 areas, 27 of them are infested with bed bugs. Infestation is scattered throughout the city, but its focus is on the east of the Karun River. The most bed bug infestation was in Asiabad followed by Manbaab areas. Prevalence of bed bug infestation estimated 9.61% in Ahvaz city. It was 5.4% and 11.6% in apartments and single houses, respectively. The lowest and highest infestation rates based on its source were 1.35% and 9.03% in wallpaper and cracks and crevices, respectively. Bedroom and sitting room were the main harborages for bed bug in the houses. The majority of residents who had bitten by a bed bug showed various allergic reactions like redness skin, papules, vesicles, pustules and blisters. Most of the people in the infested houses (62%) were very concern about bed bug infestation.
Public education and increasing the knowledge of people can lead to successful management, prevention and elimination of this nuisance pest.
Public education and increasing the knowledge of people can lead to successful management, prevention and elimination of this nuisance pest.
Leishmaniases are parasitic diseases caused by
species and transmitted by the bite of sand flies. The genus
and
of sand flies are known to be the responsible vector for transmitting almost all
species to humans. The detection of
DNA in species of the genus
, in different regions, suggests their likely role in
transmission.
Our objective was to determine the potential geographical distribution of
the most dominant
species in Morocco, using ecological niche modeling.
The results showed the widespread geographical distribution of
in Morocco, specifically in northern and central Morocco where visceral and cutaneous leishmaniasis foci occur. There were six abiotic factors affecting the distribution of
whose annual precipitation, precipitation seasonality and precipitation of driest month as the most important ecological variables of the model.
A positive statistical correlation between human leishmaniasis cases and
abundance was noted suggesting the potential involvement of
in local
transmission cycles.
