Caseywall1507

The structure of the polysaccharide O-chain of the lipopolysaccharide isolated from the sequenced strain Chromobacterium violaceum ATCC 12472 (NCTC 9757) was investigated by chemical and NMR analyses, and concluded to be -4-α-Leg5Ac7Ala-4-β-d-ManNAlaA3OAc-3-α-d-GlcNAc-where Leg5Ac7Ala indicates 5-acetamido-7-alanylamido-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulopyranosonic acid and ManNAlaA3OAc 3-O-acetyl-2-alanylamido-2-deoxymannopyranuronic acid. The structure of the core with one repeating unit of the polysaccharide attached was also analyzed, and it was found that the O-chain polysaccharide is linked to the core via β-GlcpNAc, as opposite to α-GlcpNAc inside the O-chain.

A colorimetric microassay for the quantitative determination of galactose in the blood was taken and updated. This method helps in diagnosis and follow-up of several inherited metabolic diseases connected to galactose metabolism deficiency such as galactosemia, glycogenosis, glycosylation, tyrosinemia and citrin deficiency. Galactose assay in the blood presents difficulties due to interference with glucose. Proteasomal inhibitor In this study, we update a method to get around these difficulties.

This procedure was based on the incubation of whole blood with orcinol in a strongly acidic solution to form a galactose and glucose complexes able to absorb at two different wavelengths.

The standard curve analysis for the individual solutions of these two sugars showed a wide range of linearity from 0 to 200 mg / l. Under optimal experimental conditions, the stirring time of the orcinol is 3 minutes, the heating time of the reaction is 20 minutes at 56 ° C, and the duration of the incubation in the dark is 40 minutes. The analysis is carried out on fresh blood. The maximum absorbance of galactose and glucose is respectively 569 nm and 421 nm. An adapted diagnosis algorithm was developed based on our results.

this method could help in screening and identifying patients with hypergalactosemia that need further investigations. It could represent a promising method for neonatal screening in countries with limited resources.

this method could help in screening and identifying patients with hypergalactosemia that need further investigations. It could represent a promising method for neonatal screening in countries with limited resources.The stability and thermodynamic properties of an enzyme are the main factors that governing its applications in industry. With that intention we have immobilized the α-amylase onto synthesized chitosan-magnetite (CSM) composite and its modified forms by gluteraldehyde (CSM-GLA), glyoxal (CSM-GLY) and epichlorohydrin (CSM-ECH). In this study all the immobilized enzymes exhibited improved pH stability about 60-80% of relative activity at pH 9 compared to the free enzyme. The temperature stability at 60 °C is up to 50% of relative activity for covalently immobilized enzymes as enzyme become more rigid by covalent binding and so protected from the conformational changes caused by the environment. The thermal deactivation of the free and immobilized enzymes follows the first order kinetics. The t1/2 and D-values were prolonged considerably in case of covalently immobilized enzymes, indicating better thermal stability than that of free and adsorbed ones. The Ed values 18.71, 32.00, 27.19 and 20.46 KJmol-1 for CSM-E, CSM-GLY-E, CSM-GLA-E and CSM-ECH-E described the high stability and resistance to heat inactivation. The Km values 0.525 ± 0.04, 0.57 ± 0.06, 0.65 ± 0.04 mg/mL and Vmax values 25 ± 0.06, 19.6 ± 0.02, 16.39 ± 0.01 μmol mg-1 min-1for CSM-GLY-E, CSM-GLA-E and CSM-ECH-E showing better substrate affinity. The immobilized enzymes have exhibited about 60% of relative activity after 90 days of storage and very good reuse potential.Four contact models including Hertz, Jamari, Jeng-Wang and Weng-Tang-Zhou-Zhu were considered. To verify the results of contact mechanics, the indentation depth has been compared with spherical geometry. According to the obtained results, by comparing the spherical and elliptical geometries, the indentation depth for spherical geometry is higher than for the ellipsoidal geometry, due to the existence of eccentricity in elliptical contact models which does not exist in spherical geometry. Among the models, the Jeng-Wang and Weng-Tang-Zhou-Zhu are models that are based on the contact of a particle with the substrate. Based on the existing experimental work, Jeng-Wang is the most suitable model for being applied in particle-substrate contact point. The Hertz model has been developed from crowned cylinder equations in order to be implemented for ellipsoidal geometry, moreover the Jamari model investigates two ellipses in contact with each other. By comparing Jeng-Wang and Weng-Tang-Zhou-Zhu models it is obvious that Jeng-Wang has the highest indentation depth while for the Weng-Tang-Zhou-Zhu model it is the lowest. These trends happen for two contact points. Also four materials are compared, among which graphite and chromium have the highest and the lowest indentation depths, respectively. Finally based on the obtained results Jeng-Wang and Jamari are the most suitable contact models that can be implemented in the manipulation of ellipsoidal nanoparticles.The purpose of this study was to use polynomial regression analyses to examine the torque- and power-velocity relationships and calculate and compare the vertices of these nonlinear models, and how they relate to measurements of muscle size and maximal strength, between male and female children and adolescents during maximal isokinetic leg extension muscle actions. Sixteen children (n = 8 males, n = 8 females) and 22 adolescents (n = 11 males, n = 11 females) participated in this study. Measurements of growth included age, maturity offset, height, body mass, fat-free mass, and quadriceps femoris muscle cross-sectional area (CSA). Participants completed maximal voluntary isometric contractions (MVICs) of the leg extensors and maximal voluntary isokinetic leg extensions at 60, 120, 180, 240, and 300°·s-1. Variables calculated during all leg extension muscle actions included peak torque (PT, Nm) and mean power (MP, W). Polynomial regression analyses determined the model of best fit for the PT- and MP-velocity relationships.