Carstensenward6656

icant toxicity was observed in animals after 28 days. In view of the in vitro cytotoxicity and genotoxicity detected, further studies are necessary to establish the safe use of CAE-Cp.For the audiological assessment of the speech-in-noise abilities of children with normal or impaired hearing, appropriate test materials are required. However, in Denmark, no standardized materials exist. The purpose of this study was to develop a Danish sentence corpus suitable for testing school-age children. Based on the 600 validated test sentences from the Danish DAT (Dagmar, Asta, or Tine) corpus, 11 test lists comprising 20 sentences each were carefully constructed. These lists were evaluated in terms of their perceptual similarity and reliability with a group of 20 typically developing, normal-hearing children aged 6 to 12 years. Using stationary speech-shaped noise and diotic stimulus presentation, speech recognition thresholds (SRTs) were measured twice per list and participant at two separate visits. click here The analyses showed that six test lists were perceptually equivalent. These lists are characterized by a grand average SRT of -2.6 dB signal-to-noise ratio, a test-retest improvement of 0.6 dB, and a within-subject standard deviation of 1.1 dB signal-to-noise ratio. The other lists were characterized by slightly higher SRTs, slightly larger training effects, and slightly larger measurement uncertainty, but were otherwise also usable. Overall, it is therefore concluded that the developed corpus is suited for assessing speech recognition in noise in Danish 6- to 12-year olds. The corpus is publicly available.The reviews of this paper are available via the supplemental material section.Background. To determine face and construct validity for the new Bimanual Fundamentals curriculum for the Simendo® Virtual Reality Laparoscopy Simulator and prove its efficiency as a training and objective assessment tool for surgical resident's advanced psychomotor skills. Methods. 49 participants were recruited 17 medical students (novices), 15 residents (intermediates), and 17 medical specialists (experts) in the field of gynecology, general surgery, and urology in 3 tertiary medical centers in the Netherlands. All participants performed the 5 exercises of the curriculum for 3 consecutive times on the simulator. Intermediates and experts filled in a questionnaire afterward, regarding the reality of the simulator and training goals of each exercise. Statistical analysis of performance was performed between novices, intermediates, and experts. Parameters such as task time, collisions/displacements, and path length right and left were compared between groups. Additionally, a total performance score was calculated for each participant. Results. Face validity scores regarding realism and training goals were overall positive (median scores of 4 on a 5-point Likert scale). Participants felt that the curriculum was a useful addition to the previous curricula and the used simulator would fit in their residency programs. Construct validity results showed significant differences on the great majority of measured parameters between groups. The simulator is able to differentiate between performers with different levels of laparoscopic experience. Conclusions. Face and construct validity for the new Bimanual Fundamental curriculum for the Simendo virtual reality simulator could be established. The curriculum is suitable to use in resident's training programs to improve and maintain advanced psychomotor skills.This study is about proposing a vaccine for all four strains of dengue virus (DENV) that could be an important approach for reaching the WHO goal of reducing dengue morbidity and mortality. The significance of the DENV envelope proteins III lies in the fact that it elicits an immune response and hence can be a potential vaccine design candidate. This domain appears to play a key role in the host cell receptor binding for viral entry and in inducing long lasting protective immunity against the infection. We used long molecular dynamic simulation and mutagenesis scanning methods to provide the dynamic environment and propose the potential mutation that may result in enhancing the binding specificity and affinity of the antigen-antibody (Ag-Ab) complex. The binding free energetics were also estimated using free energy perturbation method. One charged mutation that is theorinine 93L to arginine interacting with epitopic glutamic acid 368 strongly contributing in increasing the binding affinity as well as specificity, predicted as -9.6 kcal/mol gain in 2H12-Fab with dengue envelope domain III binding free energy relative to the wild-type. In conclusion, the one charged residue that showed theoretically enhances the binding affinity of Ag-Ab complex by making couple of interactions i.e. by substituting theorinine to arginine in the antibody chains and can be considered as potential dengue vaccine candidate. Communicated by Ramaswamy H. Sarma.

Declines in gait parameters are common with aging and more pronounced in tasks with increased executive demand. However, the neural correlates of age-related gait impairments are not fully understood yet.

To investigate (

) the effects of aging on prefrontal cortex (PFC) activity and gait parameters during usual walking, obstacle crossing and dual-task walking and (

) the association between PFC activity and measures of gait and executive function.

Eighty-eight healthy individuals were distributed into 6 age-groups 20-25 (G20), 30-35 (G30), 40-45 (G40), 50-55 (G50), 60-65 (G60), and 70-75 years (G70). Participants walked overground under 3 conditions usual walking, obstacle crossing, and dual-task walking. Changes in oxygenated and deoxygenated hemoglobin in the PFC were recorded using functional near-infrared spectroscopy. Gait spatiotemporal parameters were assessed using an electronic walkway. Executive function was assessed through validated tests.

Between-group differences on PFC activity were observed for all conditions. Multiple groups (ie, G30, G50, G60, and G70) showed increased PFC activity in at least one of the walking conditions. Young adults (G20 and G30) had the lowest levels of PFC activity while G60 had the highest levels. Only G70 showed reduced executive function and gait impairments (which were more pronounced during obstacle crossing and dual-task walking). PFC activity was related to gait and executive function.

Aging causes a gradual increase in PFC activity during walking. This compensatory mechanism may reach the resource ceiling in the 70s, when reduced executive function limits its efficiency and gait impairments are observed.

Aging causes a gradual increase in PFC activity during walking. This compensatory mechanism may reach the resource ceiling in the 70s, when reduced executive function limits its efficiency and gait impairments are observed.Measuring attitudes and behaviors associated with sexual consent is crucial to research addressing sexual violence, as lack of consent is a defining feature thereof. This research developed and validated the Process-Based Consent Scale to address concerns associated with missing components of existing consent scales. The factor structure was supported by an exploratory factor analysis (EFA) and subsequent confirmatory factor analysis (CFA). The scale contains three subscales ongoing consent, communicative sexuality, and subtle coercion. Results indicated good convergent and concurrent validity. Future research should consider using this tool to measure attitudes and behaviors related to sexual consent and coercion.Male-to-female sex reversal in horses is a developmental disorder in which phenotypic females have a male genetic constitution. Male-to-female sex reversal is the second most common genetic sex abnormality, after X chromosome monosomy. All male-to-female sex reversal cases studied to date have been found to be infertile. Therefore, a screening test is particularly useful in laboratories doing DNA genotyping in horses. Our laboratory has tested > 209,000 horses for parentage using a panel of microsatellite markers and the sex marker gene amelogenin (AMEL). Suspect XY sex reversal cases are reported females with a male profile by AMEL testing. After routine genotyping, 49 cases were detected and further tested using the sex-determining region Y (SRY) gene, confirming the XY SRY-negative genotype of suspect sex reversal cases. When some inconsistencies arose in the initial result, a molecular panel of X- and Y-linked markers was analyzed for these samples. Of the 49 cases, 33 were confirmed as XY SRY-negative. The remaining 16 cases were identified as false-positives as a result of anomalies of AMEL testing in horses.Research in theoretical quantum gravity has continued expansively even as it has become detached from classic arbiters of research such as direct empirical falsification. This makes it an interesting test case for theories of what motivates and mediates contemporary scientific research and of the nature of scientific objectivity. We conducted 50 semi-structured interviews with researchers in the rival camps of string theory and loop quantum gravity, coded a subset for reoccurring themes, and subjected the resulting data to statistical analysis. To delineate the subjective tastes and the related process of collective consensus-making in contemporary quantum gravity research, we mobilize aspects of Daston and Galison's depiction of the scientific self and its relation to epistemic virtues, Bourdieu's field-centered account of social space, and Kantian notions of aesthetics. We make two key contributions. First, our analysis sheds light on the inner workings of the field by connecting its internal epistemic struggles with approaches to understanding scientific fields. Second, our application of theories of social reproduction to the substance of scientific inquiry allows some substantive generalizations of Daston and Galison's framework.Abnormal foot motion is considered to be related to sports related injuries. This study aimed to identify the relationship between calf muscle activity and inter-segment coordination of the foot during single-leg drop jumps. Eleven healthy men participated and performed single-leg drop jumps from a 30-cm box. Muscle activity of the tibialis posterior (TP), flexor digitorum longus, peroneus longus (PL) and gastrocnemius were measured. The rearfoot and midfoot segment angle from landing to leaping were calculated according to the Rizzoli Foot Model and time scaled to 100%. A modified vector coding technique was employed to classify inter-segment coordination of every 1% into four patterns (in-phase, anti-phase, rearfoot phase,and midfoot phase). The relationship between percentage of each pattern and muscle activity levels were statistically analysed with correlation coefficient. The TP showed a significant positive correlation with percentage of in-phase in coronal plane (r = 0.61, p = 0.045). The PL also showed a trend of positive correlation to in-phase in coronal plane (r = 0.59, p = 0.058). TP and PL muscle activities may modulate the inter-segment coordination between the rearfoot and midfoot in coronal plane. Clinically, these muscles should be assessed for abnormal inter-segment foot motion.