Carrmartin9077

Annexin V/PI assay showed that the apoptotic cells were 0.75, 5.8, 12.4 and 22.66% at 0,7,14 and 28 µM concentrations of Withaferin-A. Withaferin-A also caused increase in the ROS and decrease in the MMP levels of the SCC-4 cells. Western blot analysis showed that the expression of LC3B II increased while of p62 decreased remarkably upon treatment with Withaferin-A, suggestive of autophagic cell death. Cell cycle analysis by flow cytometry showed that Withaferin-A caused increase in the G2/M phase cells triggering arrest of cancer cells at the G2/M checkpoint of the cell cycle. Finally, western blot analysis showed that Withaferin-A blocked the MAPK/RAS/RAF signalling pathway in the SCC-4 cells. CONCLUSIONS These results suggest that Withaferin-A may prove a potent lead molecule in oral cancer treatment and warrants further investigations.PURPOSE This study aimed to compare between the clinical efficacy of laparotomy and laparoscopic radical resection of gastric cancer and their effects on C-reactive protein (CRP), carcinoembryonic antigen (CEA) and insulin resistance. METHODS 210 patients with gastric cancer admitted to Dongying People's Hospital from September 2013 to July 2015 were included in this study. The patients were divided according to surgery type into the laparotomy group (n = 104) and the laparoscopy group (n = 106). The operative time, intraoperative bleeding, lymph node dissection, postoperative exhaust time and postoperative complications were recorded. Peripheral blood CRP and CEA levels were measured by enzyme-linked immunosorbent assay (ELISA). Fasting blood glucose (FBG), AND fasting insulin (FINS) levels were measured before operation and 1, 3 and 7 days after operation. All patients were followed up by telephone and letters for 5 years. The patients in the two groups were investigated by a quality of life questionnaire. RESULTS The intraoperative bleeding and postoperative exhaust time in THE laparoscopy group were significantly lower than those in the traditional laparotomy group, while the operative time and the number of lymph node dissections were higher. The CRP and CEA in the laparoscopy group were significantly lower than in the laparotomy group on the 1ST, 2ND and 3RD day after operation (p less then 0.05). The FBG, FINS and HOMA-IR in the laparoscopy group were significantly lower than those in the laparotomy group on the 1ST and 3RD day after operation (p less then 0.05). The scores of quality of life in the laparoscopy group were lower than those in the laparotomy group (p less then 0.05). CONCLUSION In conclusion, laparoscopic radical resection of gastric cancer can reduce the levels of CRP, CEA and insulin resistance, while the degree of inflammation and insulin resistance after laparoscopy is lower than that after laparotomy, which is beneficial to postoperative recovery.PURPOSE Nodal status represents probably the most important determinant of gastric cancer prognosis. The purpose of the present study was to assess the impact of the primary tumor's T stage on lymph node harvesting after D1 resections for gastric cancer. METHODS Between January 2000 and January 2012, the medical files of patients who presented to our department with the diagnosis of gastric cancer and were submitted to a gastric resection with curative intent were retrospectively reviewed. check details A total of 134 gastric cancer patients (mean age 67.36±9.64 - 35 females and 99 males) were submitted to a gastrectomy in our department (total or subtotal) with curative intent. The distribution of the tumors within the stomach was as follows upper third - 37 patients, middle third - 49 patients and lower third - 46 patients. RESULTS Lymph node retrieval was superior in advanced T stage patients (T3,T4a/T4b) compared to their low T stage (T1a/T1b,T2) counterparts (p=0.0008). Similar findings were encountered when the comparison was reduced to the subtotal gastrectomy subgroup (p=0.0047). However, although there was a distinct trend, statistical significance was not reached for the patient group submitted to total gastrectomy (p=0.1088). CONCLUSION The results of the present study seem to add another value i.e. tumor's T stage in the equation of lymph node retrieval in gastric cancer resection specimens. Lymph node retrieval in gastric cancer patients appeared to be dependent to the primary tumors T stage in the given patient sample.PURPOSE Gastric cancer accounts for considerable mortality across the globe. In this study the anticancer effects of a natural compound Berberine were investigated in vitro. Effects of berberine on cell migration, cellular apoptosis, Nf-kB and JNK/p38 signalling pathways were also studied. METHODS The cell viability of SNU-1 gastric cancer cells after berberine treatment was evaluated by CCK-8 assay, while the effects on cell migration were checked by wound healing assay. Effects on cellular apoptosis were evaluated by fluorescence microscopy using DAPI staining, as well as using flow cytometry with annexin V/propidium iodide (PI) staining. Effects on apoptosis-related protein expressions were checked by western blot method. RESULTS The results showed that Berberine decreased the viability of the gastric cancer SNU-1 cells and exhibited an IC50 of 30 µM. The cytoxicity of Berberine was also investigated on the normal GES-1 gastric cells and it was found that Berberine exerted very low toxic effects on these cells and exhibited an IC50 of 120 µM. Berberine also caused remarkable changes in the morphology of the SNU-1 cells. PI and DAPI staining revealed that Berberine prompted apoptosis of the SNU-1 cells in a dose-dependent manner. The apoptotic cells increased from 2.2% in control to around 35% at 30 µM concentration. Berberine also suppressed the migration and invasion of the gastric cancer cells via blocking of the JNK/p38 signalling pathway. CONCLUSIONS Berberine may act as a promising drug candidate for gastric cancer as demonstrated from the current study.PURPOSE Studies have shown that Phloretin exerts anticancer effects on several types of cancer cells. Nonetheless, the anticancer effects of Phloretin have not been fully explored against the human gastric cancer cells. Therefore, this study was undertaken to evaluate the anticancer effects of Phloretin against the human gastric cancer cells. METHODS Cell proliferation was evaluated by WST-1 assay while cell cycle analysis was carried out by flow cytometry. The effects on cell migration and invasion were evaluated by wound healing assay and transwell assays, respectively. Electron microscopy and western blot methods were used to study effects on autophagy and ERK1/2/MAPK signalling pathway. RESULTS The results showed that Phloretin inhibited the proliferation rate of the human SNU-1 gastric cancer cells and showed an IC50 of 18 µM. However, Phloretin showed very high IC50 (80 µM) against the normal GES-1 normal gastric cells. Electron microscopy showed that Phloretin triggered autophagy in the SNU-1 gastric cancer cells which was accompanied by enhancement in the expression of LC3B II and Beclin 1.