Campbellwind8930

However, LY294002 attenuated the protective effect of STRAP on cardiomyocytes. CONCLUSIONS STRAP reduces ERS and apoptosis in cardiomyocytes by activating the PI3K/PDK1/Akt signaling pathway, thereby reducing myocardial MIRI.OBJECTIVE To investigate the expression of circ_0072309 in patients with ischemic stroke (IS) and in LIFR humanized mice with middle cerebral artery occlusion (MCAO). Further, we explored the underlying mechanism of circ-0072309 in IS. MATERIALS AND METHODS The content of circ-0072309 in serum of patients with IS (n = 70) was measured by qRT-PCR, and the ROC curve was analyzed. LIFR humanized mice were used to measure the content of circ-0072309 in ischemic hemisphere by qRT-PCR and the protein expression of cleaved-caspase-3, cleaved-caspase-8 were detected by Western blot. After that, the expression of miR-100 in serum of patients with IS and in ischemic hemisphere of MCAO mice were detected, and then, we analyzed the correlation between the expression of circ-0072309 and miR-100. The binding sites between circ-0072309 and miR-100 were predicted by online database. We detected whether cric-0072309 bind to miR-100 by Dual-Luciferase report in bEnd2. In addition, bEnd2 was treated with oxygen-glucose deprivat Dual-Luciferase showed that miR-100 regulated cell survival and apoptosis by directly binding to mTOR. By comparing treated bEnd2 with circ-0072309, co-transfected bEnd2 with circ-0072309 and miR-100 reduced cell survival and increased apoptosis. CONCLUSIONS According to these results, this study revealed that the circ_0072309-miR-100-mTOR regulatory axis could alleviate IS, and it may be a potential target for the treatment of IS.OBJECTIVE The aim of this study was to explore the association between the expression of mitogen-activated protein kinase (MAPK)/extracellular regulated protein kinase (ERK) pathway and neuronal apoptosis in rats with white matter lesions (WML). MATERIALS AND METHODS Sprague-Dawley (SD) rats were selected as the research objects. Rat models of ischemic WML were established by bilateral common carotid artery ligation. NSC-2260804 Subsequently, brain tissues were collected from rats in sham operation group and WML group, respectively. Hematoxylin-eosin (HE) staining assay was conducted to observe the pathological changes in white matters (WMs) (callosum, internal capsule, and optic nerve) and apoptotic cells in brain tissues. The protein expression levels of phosphorylated ERK (p-ERK) and ERK, phosphorylated MAPK (p-MAPK), and MAPK in tissues were measured by Western blotting. Immunohistochemistry was employed to detect the expression levels of p-ERK, ERK, p-MAPK, and MAPK in brain tissues of the two groups. Next, nerve ces in WML group. Besides, WML group exhibited significantly up-regulated expressions of p-ERK and p-MAPK, as well as basically unchanged expressions of ERK and MAPK (p less then 0.05). After PD was added for 1 d, 2 d, and 3 d, the MAPK/ERK pathway was repressed, which was the most significantly at 3 d. Furthermore, the anti-apoptotic phenotype of neurons was detected, which was more pronounced at 3 d (p less then 0.05). CONCLUSIONS Rats with WML exhibited elevated MAPK/ERK activity and evident apoptosis. After inhibiting the phosphorylation site of MAPK/ERK in rat neuronal cells, the expression of pro-apoptotic protein decreased, and the apoptosis was relieved. In rats with WML, neuronal apoptosis is promoted by activating the MAPK/ERK pathway, thus worsening the condition.OBJECTIVE This study aims to clarify the influences of miRNA-584 on proliferative and invasive abilities in NK/T-cell lymphoma, and to illustrate the underlying mechanism. PATIENTS AND METHODS MiRNA-584 levels in peripheral blood of NK/T-cell lymphoma patients and healthy controls were detected by qRT-PCR. Potential relationship between miRNA-584 level and clinical indicators of NK/T-cell lymphoma patients was analyzed. Kaplan-Meier curves were depicted for assessing the prognostic value of miRNA-584 in NK/T-cell lymphoma patients. After overexpression or knockdown of miRNA-584, changes in proliferative and invasive abilities in KHYG-1 and SNK-6 cells were assessed by CCK-8 and transwell assay, respectively. The potential mechanism of miRNA-584 and its downstream gene FOXO1 in regulating the development of NK/T-cell lymphoma was explored by Dual-Luciferase reporter gene assay and rescue experiments. RESULTS Peripheral blood levels of miRNA-584 were lower in NK/T-cell lymphoma patients than those of healthy control. Compared with NK/T-cell lymphoma patients expressing high level of miRNA-584, those expressing a low level suffered worse tumor staging, higher rate of bone marrow invasion, and worse prognosis. Overexpression of miRNA-584 suppressed proliferative and invasive abilities in KHYG-1 and SNK-6 cells. On the contrary, knockdown of miRNA-584 yielded the opposite results. FOXO1 was showed to be the direct target binding miRNA-584, and its level was negatively regulated by miRNA-584. Notably, FOXO1 could partially abolish the regulatory effects of miRNA-584 on proliferative and invasive abilities in NK/T-cell lymphoma. CONCLUSIONS Low level of miRNA-584 is closely linked to advanced stage, susceptibility to bone marrow invasion, and poor prognosis in NK/T-cell lymphoma. MiRNA-584 suppresses proliferative and invasive abilities in NK/T-cell lymphoma by targeting FOXO1.OBJECTIVE To explore the association between c-myc and K-ras gene polymorphisms and non-Hodgkin lymphoma (NHL). PATIENTS AND METHODS A total of 200 NHL patients in our hospital in the past 3 years were collected as disease group, while 200 healthy people were taken as control group. The genomic deoxyribonucleic acid (DNA) in the peripheral blood was extracted in both groups, amplified via Polymerase Chain Reaction (PCR) and sent to the company for the detection of c-myc and K-ras gene polymorphisms. The expressions of c-myc and K-ras were detected via Reverse Transcription-quantitative PCR (RT-qPCR), and the levels of clinical indexes hemoglobin (Hb), platelet (PLT) and lactate dehydrogenase (LDH) were determined in the Laboratory Department. RESULTS The allele distribution at c-myc gene locus rs121918684 was different between control group and disease group (p=0.000), and the G allele frequency was 202 (0.505) in the control group and 263 (0.657) in the disease group. In the disease group, the GG genotype frequency at c-myc gene locus rs121918684 [97 (0.