Burnettsheehan5958

Treatment with Artemisinin (50 mg/kg) reduced the levels of PAP, LDH, prostate weight and prostatic index to a significant extent and restored the histoarchitectural features of the cells. Conclusion The present study concludes that the Artemisinin is efficacious in testosterone propionate induced BPH. This could be attributed, at least partly, to its anti-inflammatory property or its role in testosterone level reduction or as a Vitamin D receptor modulator.Background Finding a safe and effective vaccine against HIV-1 infection is still a major concern and highly valuable. Objective This study aimed to design and produce a recombinant Nef-MPER V3 protein fused with IMT-P8, using E. coli expression system to provide a potential HIV vaccine with high cellular penetrance. Methods After synthesizing the DNA sequence of the fusion protein, the construct was inserted into pET-28 expression vector. The recombinant protein expression was induced using 1 mM IPTG and the product was purified through affinity chromatography. Characterization of cellular delivery, toxicity and immunogenicity of the protein was carried out. Results The recombinant protein was expressed and confirmed by anti-Nef antibody through western blotting. Data analyses showed that the protein has no considerable toxicity effect and has improved the IMT-P8 penetration rate in comparison with a control sample. Moreover, the antigen immunogenicity of the protein induced specific humoral response in mice. Conclusion It was concluded that IMT-P8- Nef-MPER-V3 fusion protein has a high penetrance rate into mammalian cell line and low toxicity for potential application as a vaccine against HIV-1.Background The anticancer effects of Phyllanthus amarus extract on various cancer cells have been investigated, however, effect of its major constituents on HCT116 human colorectal cancer cells has not been reported. Objective In the present study, we investigated the cytotoxic effect of 80% ethanol extract of P. amarus and its marker constituents (phyllanthin, hypophyllanthin, gallic acid, niranthin, greraniin, phyltetralin, isolintetralin, corilagin and ellagic acid) on HCT116 and their underlying mechanisms of action. Method Their anti-proliferative and apoptotic effects on HCT 116 were performed using MTT assay and flow cytometric analysis, respectively, while caspases 3/7, 8 and 9 activities were examined using colorimetric method. The expression of cleaved poly ADP ribose polymerase enzyme (PARP) and cytochrome c proteins was investigated by immune-blot technique. Results HPLC and LC-MS/MS analyses demonstrated the extract contained mainly lignans and polyphenols. The plant samples markedly suppressed the growth and expansion of HCT116 cells concentration- and time-dependently with no toxicity against normal human fibroblast CCD18 Co. P. amarus extract, phyllanthin and gallic acid induced mode of cell death primarily via apoptosis as confirmed by the exteriorization of phosphatidylserine. Caspases 3/7, 8, and 9 activities increased concentration-dependently following 24 h treatment. The expressions of cleaved PARP (Asp 214) and cytochrome c were markedly upregulated. Conclusion P. amarus extract, phyllanthin and gallic acid exhibited apoptotic effect on HCT116 cells via caspases-dependent pathway.Background Home parenteral nutrition (HPN) is a lifesaving clinical care process. However, undetected hazards and vulnerabilities in care transitions from hospital to community care may pose risk to patient safety. learn more Avoidable complications and adverse events may hinder the benefits of treatment. Objective The analysis carried out aims at framing through human factors and ergonomics (HF/E) the critical issues for patient safety related to clinical care practices for HPN in healthcare organization. Methods We present the results of a proactive risks assessment analysis based on the FMEA methodology (Failure Mode and Effects Analysis) carried out in three different areas of the regional health care system of Tuscany, Italy. The clinical risk management and patient safety unit assessed the risk perception of healthcare workers (HWs) in regard to patient safety and situational awareness throughout the HPN patient journey. Results The analysis revealed heterogeneity in the Risk Priority Index (RPI) expressed by HWs.proved awareness of the criticalities and the role of nutrition units throughout the care process.Background With the improvements in living standards, height is getting more attention. Malnutrition is one of the main causes of children's short stature, therefore nutritional intervention in adolescence is the key to prevent short stature. The peptides from Antarctic krill (AKPs), the ideal protein model, act in bone formation and anti-osteoporosis. However, the studies on promoting longitudinal bone growth by AKPs have not been reported. Methods Three-week-old male ICR mice, to construct the adolescent mice model, randomly divided into three groups normal group, casein group (casein, 300 mg/kgꞏBW), and AKPs group (AKPs, 300 mg/kgꞏBW). After 21 days of drugs administration, the effects of AKPs on serum biochemical indexes and femur histomorphology of mice, and the mechanism of AKPs promoting longitudinal bone growth was discussed. Results AKPs significantly increased the longitudinal bone growth and improved bone strength. In addition, AKPs remarkably promoted proliferation and hypertrophy of chondrocytes in the growth plate. The further mechanism revealed that AKPs increased serum growth hormone (GH) and insulin-like growth factors-1(IGF-1) contents, which activated the downstream GH/IGF-1 axis signaling pathways. Moreover, AKPs induced the secretion and expression of bone morphogenetic protein 2 (BMP-2) and triggered the activation of BMP2-dependent Smads signaling. AKPs also activated Wnt/βcatenin signaling, and synergistically activated the expression of runt-related transcription factor 2 (Runx 2) and osterix (OSX). Conclusion AKPs promoted longitudinal bone growth by activating GH/IGF-1 axis, BMP-2/Smads and Wnt/β-catenin pathways, suggesting AKPs to be a potential nutrient fortifier for longitudinal bone growth.