Bryanlindgren0145

To analyze the inflammatory millieu in oral squamous cell carcinoma (OSCC) tumors and the influence of macrophages related-cytokines on the tumor cell migration.

Inflammatory protein profile and macrophage population (M2/M1 ratio) of human OSCC fragments were analyzed by proteomic analysis and flow cytometry assay respectively. To evaluate the effects of inflammation on OSCC behavior, we analyzed the role of polarized macrophages and cytokines (IL-6, IL-1β and TNF-α) on OSCC cell lines (SCC25 and Cal27) responsiveness by western blotting (cell signaling) and time-lapse (cell migration). Also, it was addressed the crosstalk of IL-6-STAT3 axis with cell migration signaling using a STAT3 inhibitor (Stattic®) and a pull down assay for the RhoGTPase Rac1 activity.

It was observed a ~2 fold predominance of M2 over M1 macrophages and a pro-inflammatory state in OSCC fragments. The M2 conditioned media increased migration speed and directionality of highly invasive OSCC cells (SCC25). OSCC cell lines were responsive to cytokine stimuli (IL6, IL-1β and TNF-α), but only IL-6 increased migration properties of OSCC cells. This effect was dependent on STAT3-phosphorylation levels, which interfered with Rac1 activation levels.

Our results suggest that the inflammatory milieu might favor invasion and metastasis of OSCC by the direct effect of macrophage-related cytokines on tumor migration.

Our results suggest that the inflammatory milieu might favor invasion and metastasis of OSCC by the direct effect of macrophage-related cytokines on tumor migration.Visual-motor illusion (VMI) is to evoke a kinesthetic sensation by viewing images of oneself performing physical exercise while the body is at rest. Previous studies demonstrated that VMI activates the motor association brain areas; however, it is unclear whether VMI immediately alters the resting-state functional connectivity (RSFC). This study is aimed to verify whether the VMI induction changed the RSFC using functional near-infrared spectroscopy (fNIRS). The right hands of 13 healthy adults underwent illusion and observation conditions for 20 min each. Before and after each condition, RSFC was measured using fNIRS. After each condition, degree of kinesthetic illusion and a sense of body ownership measured using the Likert scale. A2ti-2 research buy Our results indicated that, compared with the observation condition, the degree of kinesthetic illusion and the sense of body ownership were significantly higher after the illusion condition. Compared with the observation condition, RSFC after the illusion condition significantly increased brain areas associated with kinesthetic illusion, a sense of body ownership, and motor execution. In conclusion, RSFC has become a biomarker that shows changes in brain function occurring due to VMI. VMI may be applied to the treatment of patients with stroke or orthopedic diseases.We are all saints and sinners Some of our actions benefit others, while other actions lead to harm. How do people balance moral rights against moral wrongs when evaluating others' actions? Across 9 studies, we contrast the predictions of three conceptions of intuitive morality-outcome-based (utilitarian), act-based (deontologist), and person-based (virtue ethics) approaches. These experiments establish four principles Partial offsetting (good acts can partly offset bad acts), diminishing sensitivity (the extent of the good act has minimal impact on its offsetting power), temporal asymmetry (good acts are more praiseworthy when they come after harms), and act congruency (good acts are more praiseworthy to the extent they offset a similar harm). These principles are difficult to square with utilitarian or deontological approaches, but sit well within person-based approaches to moral psychology. Inferences about personal character mediated many of these effects (Studies 1-4), explained differences across items and across individuals (Studies 5-6), and could be manipulated to produce downstream consequences on blame (Studies 7-9); however, there was some evidence for more modest roles of utilitarian and deontological processing too. These findings contribute to conversations about moral psychology and person perception, and may have policy and marketing implications.We explored the acquisition of three types of second language (L2) words in a paired-associates learning task. Seventy-six Polish participants were presented with 24 nonwords paired with pictures; they completed 8 interleaving test blocks of form production and meaning recognition, both followed by feedback. The nonwords included "cognates" (nonwords resembling the Polish word for the object depicted in a given picture), "false cognates" (resembling a different Polish word than depicted), and "non-cognates" (nonwords different from Polish words). We measured the learning trajectories for all word types across the blocks. Cognates were fastest to learn in the recognition blocks as well as in the production blocks. Compared to non-cognates, false cognates were learned equally fast in the recognition blocks, but faster in the production blocks. This suggests the learning of false cognates benefits from the overlap in L1-L2 form and is not harmed by L1 interference, while the learning of cognates benefits from both form overlap and conceptual overlap. The study is unique as it examines how learners acquire both the form of new words, and the link between the L2 forms and their meanings. It also explores the dynamics of the learning process.Human attention is subject to fluctuations. Mind-wandering (MW) - attending to thoughts unrelated to the current task demands - is considered a ubiquitous experience. According to the Control Failure x Concerns view (McVay & Kane, 2010), MW is curbed by executive control, and task-irrelevant thoughts enter consciousness due to attentional control lapses. The generation of off-task thoughts is assumed to increase with higher number of personal concerns. Challenging this view, older adults report less MW than younger adults. Here, we addressed the hypothesis that older adults report less MW due to a lower ability to notice attention lapses and to appraise their current on-task focus. In an age-comparative study (N = 40 younger and N = 44 older adults) using a battery of three tasks spanning working memory, reading comprehension, and sustained attention, we assessed the correlation between the degree of self-reported on-task focus and task performance on a trial-by-trial basis. Younger and older adults' degree of on-task attention measured through thought probes was correlated equally strongly with performance across trials in all tasks, indicating preserved ability to monitor attentional fluctuations in healthy aging.