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The cavitation and mechanical of ultrasound have effects on the self-assembly process and collagen gel structure and digestion characteristics, which is of great significance for the development of the chicken industry and collagen market.Electric fields (EF) technologies have been establishing a solid position in emergent food processing and have seen as serious alternatives to traditional thermal processing. During the last decades, research has been devoted to elucidation of technological and safety issues but also fundamental aspects related with interaction of electric fields (EF) with important macromolecules, such as proteins. Proteins are building blocks for the development of functional networks that can encompass health benefits (i.e. nutritional and bioactive properties) but may be also linked with adverse effects such as neurodegenerative diseases (amyloid fibrils) and immunological responses. The biological function of a protein depends on its tridimensional structure/conformation, and latest research evidences that EF can promote disturbances on protein conformation, change their unfolding mechanisms, aggregation and interaction patterns. This review aims at bringing together these recent findings as well as providing novel perspectives about how EF can shape the behavior of proteins towards the development of innovative foods, aiming at consumers' health and wellbeing.The use of ingredients based on plant protein isolates is being promoted due to sustainability and health reasons. Proxalutamide purchase However, it is necessary to explore the behaviour of plant protein isolates during gastrointestinal digestion including the profile of released free amino acids and the characterization of resistant domains to gastrointestinal digestion. The aim of the present study was to monitor protein degradation of four legume protein isolates garden pea, grass pea, soybean and lentil, using the harmonized Infogest in vitro digestion protocol. In vitro digests were characterized regarding protein, peptide and free amino acid content. Soybean was the protein isolate with the highest percentage of insoluble nitrogen at the end of the digestion (12%), being this fraction rich in hydrophobic amino acids. Free amino acids were mainly released during the intestinal digestion, comprising 21-24% of the total nitrogen content, while the percentage of nitrogen corresponding to peptides ranged from 66 to 76%. Legume globulins were resistant to gastric digestion whereas they were hydrolysed into peptides and amino acids during the intestinal phase. However, the molecular weight (MW) distribution demonstrated that all intestinal digests, except those from soybean, contained peptides with MW > 4 kDa at the end of gastrointestinal digestion. The profile of free amino acids released during digestion supports legume protein isolates as an excellent source of essential amino acids to be used in protein-rich food products. Peptides released during digestion matched with previously reported epitopes from the same plant species or others, explaining the ability to induce allergic reactions and cross-linked reactivity.This study aimed to evaluate the effects of metal salts on α-amylase-catalyzed hydrolysis of broken rice under a moderate electric field (MEF) by monitoring changes in hydrolysis efficiency, temperature, α-amylase activity, starch-metal ion interaction, and the structural and physicochemical properties of hydrolysates. Results showed that metal salts affected the hydrolysis mainly by altering α-amylase activity rather than by inducing thermal effect or interacting with starch. Reducing sugar content reached 125.0 g/L, while α-amylase activity increased by 18.16% when treated with 0.12 mmol/L Ca2+. Holes on hydrolysates treated with Ca2+ and Mg2+ were larger than those treated with Mn2+ and Cu2+. No M-O bond was formed after the hydrolysis. The crystallinity was slightly increased with the hydrolysis and the values for Ca2+- and Mg2+-treated samples were larger. The water and oil absorption capacity of the hydrolysate treated with Ca2+ was the highest. This study extended the knowledge of the roles of metal ions on MEF-assisted enzymatic hydrolysis and will contribute to the development of an innovative technology for starch modification.Morin and PUFAs are bioactive compounds provided by the diet, with multiple biological activities, among which are the modulation of inflammation in various chronic diseases. The effect of supplementation with Morin, PUFAs, and the mixture of both on the levels of mRNA expression of the Nlrp3 inflammasome as well as genes associated with inflammation and lipid metabolism, in an obesity model through a high-fat diet, during 8 weeks of administration were evaluated. The three treatments negatively regulated the expression of Nlrp3 mRNA. Morin showed a better effect by modulating downwards the expression of the mRNA of Il-18, Casp-1, Pparγ, and Serbp-1c, in addition to positively modulating the expression of the mRNA of Ppar-α, as well as Adiponectin. The combined treatment of Morin plus the PUFAs maintained similar levels under normal conditions for the mRNA expression of Tlr4 and Ucp2.This study evaluates the effects of intra-amniotic administration of yacon (Smallanthus sonchifolius) flour soluble extracts (YFSE) on intestinal bacterial populations, brush border membrane (BBM) functionality and morphology, by using the Gallus gallus model. The YFSE increased (p less then 0.05) relative abundance of Lactobacillus, Bifidobacterium, Clostridium and E. coli compared to 18MΩ H2O. The YFSE had systematic effect on BBM functionality, via the upregulation of zinc (zinc transporters - ZnT1, ZnT7 and ZIP9) and iron (ferroportin, Duodenal cytochrome (DcytB) transporters, sucrose isomaltase (SI), and down regulation of Interleukin 1 beta (IL1β), and hepcidin genes expression when compared to the inulin administered group. The YFSE administration increased glycogen concentrations in pectoral muscle compared to noninjected and 18 Ω H2O groups, however, did not change gene expression of enzymes related to glycolysis (phosphofructokinase) and gluconeogenesis (glucose-6 phosphatase). The YFSE increased the depth of crypts, crypt goblet cell diameter, number and type (acidic), and villi goblet cell diameter and type (acidic) when compared to all other groups.