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Conclusion The theoretical framework is a feasible and effective guide to enhance human dimensions of care. Our rigorous participative process facilitates sharing of patient and staff experience, sensitizing practitioners' understandings and helping develop new ways of providing theoretically robust person-centred care based on lifeworld approaches.The ornamental plant Camellia japonica is widely distributed worldwide and is susceptible to various environmental stresses. The WRKY transcription factor (TF) is an important node of plant tolerance. However, WRKY TFs from C. japonica have not been reported yet. In this study, 48 CjWRKYs, namely, CjWRKY1 to CjWRKY48, were identified. Protein structure analysis revealed that CjWRKY proteins contain a highly conserved motif (WRKYGQK) and two variant motifs (WRKYGKK and WRKYGRK). Phylogenetic analysis indicated that the 48 CjWRKYs can be divided into three groups, which are further classified into six subgroups, namely, I-C, II-a, II-b, II-c, II-e, and III, which contain 10, 6, 8, 13, 7, and 4 members, respectively. The expression patterns of 15 CjWRKYs under salicylic acid (SA) treatment were investigated by real-time quantitative PCR (qRT-PCR). Results showed that the 15 CjWRKYs could be induced by SA treatment. This study is the first to screen CjWRKYs and identify the expression profile of CjWRKYs under SA treatment and provides a theoretical basis for analyzing the function of CjWRKY genes to SA stress tolerance in C. japonica.We address three major questions in astronomy, namely the detection of biosignatures on habitable exoplanets, the geophysics of exoplanets and cosmology. To achieve this goal, two requirements are needed (i) a very large aperture to detect spectro-polarimetric and spatial features of faint objects such as exoplanets, (ii) continuous monitoring to characterize the temporal behaviour of exoplanets such as rotation period, meteorology and seasons. An Earth-based telescope is not suited for continuous monitoring and the atmosphere limits the ultimate angular resolution and spectro-polarimetrical domain. Moreover, a space telescope in orbit is limited in aperture, to perhaps 15 m over many decades. This is why we propose an OWL-class lunar telescope with a 50-100 m aperture for visible and IR astronomy, based on ESO's Overwhelmingly Large Telescope concept, unachievable on Earth for technical issues such as wind stress that are not relevant for a lunar platform. It will be installed near the south pole of the Moon to allow continuous target monitoring. The low gravity of the Moon will facilitate its building and manoeuvring, compared to Earth-based telescopes. Pixantrone We introduce a new original idea such a large lunar telescope will allow Intensity Interferometric measurements when coupled with large Earth-based telescopes, leading to picosecond angular resolution. Rather than going into all details, our objective is essentially to inject new ideas and give a kind of roadmap. In particular, the choice of a final location will have to find a compromise between the cool temperature of craters at the Moon South Pole and the visibility of Earth for some science objectives. This article is part of a discussion meeting issue 'Astronomy from the Moon the next decades'.Due to ionosphere absorption and the interference of natural and artificial radio emissions, astronomical observation from the ground becomes very difficult at the wavelengths of decametre or longer, which we shall refer to as the ultralong wavelengths. This unexplored part of the electromagnetic spectrum has the potential for great discoveries, notably in the study of cosmic dark ages and dawn, but also in heliophysics and space weather, planets and exoplanets, cosmic ray and neutrinos, pulsar and interstellar medium (ISM), extragalactic radio sources, and so on. The difficulty of the ionosphere can be overcome by space observation, and the Moon can shield the radio frequency interferences (RFIs) from the Earth. A lunar orbit array can be a practical first step to opening up the ultralong wave band. Compared with a lunar surface observatory on the far side, the lunar orbit array is simpler and more economical, as it does not need to make the risky and expensive landing, can be easily powered with solar energy, and the data can be transmitted back to the Earth when it is on the near-side part of the orbit. Here, I describe the discovering sky at the longest wavelength (DSL) project, which will consist of a mother satellite and 6-9 daughter satellites, flying on the same circular orbit around the Moon, and forming a linear interferometer array. The data are collected by the mother satellite which computes the interferometric cross-correlations (visibilities) and transmits the data back to the Earth. The whole array can be deployed on the lunar orbit with a single rocket launch. The project is under intensive study in China. This article is part of a discussion meeting issue 'Astronomy from the Moon the next decades'.LOUPE, the Lunar Observatory for Unresolved Polarimetry of the Earth, is a small, robust spectro-polarimeter for observing the Earth as an exoplanet. Detecting Earth-like planets in stellar habitable zones is one of the key challenges of modern exoplanetary science. Characterizing such planets and searching for traces of life requires the direct detection of their signals. LOUPE provides unique spectral flux and polarization data of sunlight reflected by Earth, the only planet known to harbour life. These data will be used to test numerical codes to predict signals of Earth-like exoplanets, to test algorithms that retrieve planet properties, and to fine-tune the design and observational strategies of future space observatories. From the Moon, LOUPE will continuously see the entire Earth, enabling it to monitor the signal changes due to the planet's daily rotation, weather patterns and seasons, across all phase angles. Here, we present both the science case and the technology behind LOUPE's instrumental and mission design. This article is part of a discussion meeting issue 'Astronomy from the Moon the next decades'.

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