Broussardrichter6487

Additionally, the geometrical features of scaffolds were examined using a scanning electron microscopy (SEM) technique. The morphology of scaffolds, dried with the aformentioned methods, was assess using SEM images to evaluate the dimensional stability of scaffolds. Chondrocyte cells cultured on the 3D-printed chitosan scaffolds dried using the air-drying technique showed high cell attachment while retaining round cellular morphology. Also, the results of the cytotoxicity test indicated that there was proper biocompatibility of the chitosan for the ATDC5 cells. Results showed that the drying method plays a decisive role in the mechanical and biological behavior of chitosan scaffolds. Considering biological and mechanical properties, the proposed 3D-printed chitosan scaffold can be of a potential structure for cartilage tissue engineering applications.Membranes based on natural polymers are highly promising therapies for skin damaged sites as they can mimic its biological microstructure to support the fibroblasts cells survival and proliferation. In addition, these membranes could be loaded with active molecules that help in skin regeneration and eliminate the potential bacterial infection. This research aims to formulate novel medicated membranes for controlled release and cytocompatibility elevation of fibroblast cells for engineering of soft tissue. Pre-formulation researches have been conducted for membranes of sodium alginate (Alg)/methyl cellulose (MC) that used loaded with undoped, Bi doped and Bi, Cu co-doped SrTiO3 using solvent casting technique. In addition, another group of these membranes were loaded with DOXycycline antibiotic (DOX) as model drug as well as for eliminating the potential bacterial infections. The prepared membranes were evaluated by XRD, SEM-EDX, FTIR, Zetasizer, and swelling behaviour was also tested. Profiles of the releasedr treatments of skin infections.High-solid chitosan matrices were prepared to investigate the effect of their swelling on structural relaxation and glass transition. Degree of crosslinking in genipin-crosslinked chitosan networks was measured with a ninhydrin assay and a suitable crosslinker concentration was determined for gels used in swelling and thermomechanical analysis. Fourier transform infrared spectroscopy and wide angle X-ray diffraction examined the intermolecular interactions, crystallinity and amorphicity of the biopolymer networks. Swelling characteristics following immersion in water included approximate equilibrium values of the average molecular weight between crosslinks and network mesh size, e.g. 902 g mol-1 and 110 nm for the preparation with an initial solids content of 60% (w/w) after 80 min of swelling, which were quantified with the modified Flory-Rehner theory. Thermal glass transition temperature was observed in the condensed crosslinked networks (≥ 70% w/w total solids) during differential scanning calorimetry experiments. Time-temperature superposition of rheological measurements, obtained with dynamic oscillation in-shear, generated a master curve describing the viscoelastic behaviour of the system, moving through the rubbery, glass transition region and glassy state. Combined analysis using the modified Arrhenius and William-Landel-Ferry theories determined mechanical glass transition temperatures in the range of -68 to -8 °C for the crosslinked biopolymer at intermediate-solid concentrations (40 to 60% w/w solids). An understanding of the effect of swelling on molecular network characteristics was achieved, which is crucial for greater control in the design of systems for the targeted delivery of bioactive compounds.Multicomponent reactions (MCRs) as a green strategy was used to crosslink carboxymethylcellulose (CMC) via the construction of bioactive carboxamide skeletons for the gentamicin (GM) oral delivery. The prepared multicomponent-crosslinked (MCC) CMC hydrogels (CMC-MCC) were fully characterized using various methods. INS018-055 research buy To explain the productivity of the prepared system, GM delivery tests performed in the simulated gastrointestinal tract (GIT) conditions. CMC-MCC could efficiently protect the loaded-GM against the acidic environment of the stomach and enhance the sustainability of drug dosing with controlling the releases in the GIT circumstances. The antibacterial performance showed that the GM-loaded CMC-MCC has notable activity against S. aureus and E. coli bacteria. Besides, the cytotoxicity study indicated that CMC-MCC toward healthy normal human umbilical vein endothelial cells (HUVEC) has a cytocompatibility nature.This work proposes the development of a starch-based drug carrier for fluoxetine (FLX) delivery and evaluate the improvement of the drug antibacterial activity. The starch nanocapsules were prepared via interfacial polyaddition reaction presenting a core-shell morphology, based on polyurethane linkage, with a particle size in the range 250-300 nm. Furthermore, FLX-loaded nanocapsules were evaluated regarding antibacterial potential against Staphylococcus aureus (ATCC® 6538P ™) and its clinical strains of methicillin-resistant. As expected, the FLX-loaded presented lower minimum inhibitory concentration (MIC) values, in the range of 190-95 μg mL-1, against all isolated microorganisms in comparison to FLX, 255 μg mL-1. According to results, the FLX-loaded starch nanocapsules have successfully improved drug antibacterial activity, generating promising perspectives on the field of the hydrophilic drug delivery systems.A study was carried out to determine the effectiveness of lignin, extracted from oil palm (Elaeis guineensis) biomass as water-in-oil (W/O) emulsifying agent. To achieve this goal, soda lignin (SL) was extracted via soda pulping process and a series of nanosized soda lignin (NSL) were prepared using homogenizer at three different speed i.e. 10,400 rpm (NSL 10), 11,400 rpm (NSL 11) and 12,400 rpm (NSL 12) for one hour. All prepared samples were characterized by FT-IR, UV-Vis spectroscopy, thermogravimetric analysis (TGA), zeta potential analyser, Transmission Electron Microscope (TEM) and Extreme High Resolution Field Emission Scanning Electron Microscope (XHR-FESEM). The result of FTIR showed that there is no prominent change occurred in spectra of all samples while a good stability was reflected by TGA curves. The percentage of creaming index and visual observations of all samples demonstrated that NSL 12 and dosage 2 g (out of 1 g, 1.5 g and 2 g) were found to be the best among all samples. Furthermore, the results of IFT indicate that NSL 12 was proven to be more stable than the commercial product.