Brochrivas8742

BACKGROUND Lung volume reduction surgery (LVRS) was introduced to alleviate clinical conditions in selected patients with heterogenous emphysema. Clarifying the most suitable patients for LVRS remained unclear. AIM This study was undertaken to specifically analyze the preoperative factor affecting to LVRS. Estradiol Benzoate clinical trial METHODS The prospective study was conducted at 103 Military Hospital between July 2014 and April 2016. Severe heterogenous emphysema patients were selected to participate in the study. The information, spirometry, and body plethysmographic pulmonary function tests in 31 patients who underwent LVRS were compared with postoperative outcomes (changing in FEV1 and CAT scale). RESULTS Of the 31 patients, there was statistically significant difference in the outcome of functional capacity, lung function between two groups (FEV1 ≤ 50% and > 50%) (∆FEV1 22.46 vs 18.32%; p = 0.042. ∆ CAT 6.85 vs 5.07; p = 0.048). Changes of the FEV1 and CAT scale were no statistically significant differences in three groups residual volume. Patients with total lung capacity less then 140% had more improved than others (∆FEV1 23.81 vs 15.1%; p = 0.031). CONCLUSION Preoperative spirometry and body plethysmographic pulmonary function tests were useful measures to selected severe heterogenous emphysema patients for LVRS. Patients with FEV1 ≤ 50%, TLC in the range of 100-140% should be selected. Copyright © 2019 Nguyen Truong Giang, Trung Nguyen Ngoc, Nguyen Van Nam, Nguyen Viet Nhung, Ta Ba Thang, Dong Khac Hung, Nguyen Duy Bac, Chu Dinh Toi, Pham Ngoc Hung.BACKGROUND β-thalassemia is one of the most common monogenic diseases worldwide. Preimplantation genetic testing (PGT) of β-thalassemia is performed to avoid affected pregnancies has become increasingly popular worldwide. In which, the indirect analysis using short tandem repeat (STRs) linking with HBB gene to detect different β-globin (HBB) gene mutation is a simple, accurate, economical and also provides additional control of contamination and allele-drop-out ADO. AIM This study established microsatellite markers for PGT of Vietnamese β-thalassemia patient. METHODS Fifteen (15) STRs gathered from 5 populations were identified by in silico tools within 1 Mb flanking the HBB gene. The multiplex PCR reaction was optimized and performed on 106 DNA samples from at-risk families. RESULTS After estimating, PIC values were ≥ 0.7 for all markers, with expected heterozygosity and observed heterozygosity values ranged from 0.81 to 0.92 and 0.53 to 0.86, respectively. One hundred percent of individuals had at least seven heterozygous markers and were found to be heterozygous for at least two markers on either side of the HBB gene. CONCLUSION In general, a pentadecaplex marker (all less then 1 Mb from the HBB gene) assay was constituted for β-thalassemia PGT on Vietnamese population. Copyright © 2019 Dang Tien Truong, Ngo Van Nhat Minh, Dinh Phuong Nhung, Hoang Van Luong, Do Quyet, Tran Ngoc Anh, Trinh The Son, Nguyen Thanh Tung, Nguyen Thi Thu Ha, Duong Thi Phuong Anh, Le Hoang, Nguyen Le Thuy, Nguyen Thi Hoa, Nguyen Duy Bac, Vu Thi Nga, Toi Chu Dinh.BACKGROUND N-methyl-d-aspartate receptor (NMDAR) antibody encephalitis appears common in the world, but the number of clinical cases in Vietnam which were recorded is rare. CASE REPORT We describe two new cases of disease in recent years with the aim of contributing to diagnosis and treatment experiences. These cases were noted over the past 3 years with the patients who have been treated at lower levels but have no results. They came to us when symptoms became worse and therefore required prolonged treatment with special intensive care facilities. The atypical and easily confused symptoms are the reasons that make the disease be detected late; leading to a much higher cost of treatment and the complication may appear in the patient. In the past, patients with these manifestations were diagnosed with unexplained encephalitis and severe sequelae or death. Autoimmune encephalitis has many types; NMDA encephalitis associated with ovarian teratoma is the most common autoimmune encephalitis in young women. CONCLUSION In conclusion, based on the case report, we hope to contribute some experiences on the diagnosis and the strategy in early treatment. With most female patients at very young age, early treatment to avoid complications will help patients have a quality life and maintain reproductive function. Copyright © 2019 Nguyen Phuong Tu, Pham Ba Nha, Nguyen Duy Hung, Nguyen Hoang Minh, Hoang Ngoc Anh, Thien Chu Dinh.BACKGROUND Spirulina platensis contains a large amount of chlorophylls, chlorophyll a, that are starting materials to synthesize functionalized chlorins. Chlorin e6 (Ce6) as well as its derivatives are second generation sensitizers using in photodynamic therapy (PDT) of various cancers. In this study, we transfer chlorophyll a of S. platensis to Ce6 derivatives and determine their several applications. AIM We aimed to evaluate the effects of Ce6 derivatives to treat cancer cells. METHODS Ce6 trimethylester was created from methyl pheophorbide a2 in S. platensis provided by the Hidumi Company, Nghe An province, Viet Nam. Hela cells were incubated with Ce6 trimethylester and the irradiated with the diode laser dose of 1.2 J/cm2/min through the system of filters £ 650 nm. MTT assay and clonogenic assay were used to determine survival rate and cloning efficiency of cells. Antimicrobial effect of Ce6 trimethylester with halogen light were studied with Propionibacterium acnes VTCC 0218 and Staphylococcus aureus VTCancer and acne. Copyright © 2019 Hoa Thi Hai Bui, Tam Thi Pham, Hien Thi Thu Nguyen, Trung Minh Do, Vu Thi Nga, Nguyen Duy Bac, Vu Thi Bich Huyen, Hai Minh Le, Quang Canh Tran.BACKGROUND Hepatocellularcarcinoma (HCC) metastasis include intrahepatic and extrahepatic metastasis. Similar to intrahepatic metastasis, extrahepatic metastases are not unusual in cases with HCC. However, colonic metastasis is infrequent. CASE REPORT We describe a clinical case, he was diagnosed with HCC a year ago, treated with TACE (transarterialchemoembolisation), re-examined with abdominal pain and defecation disorder. The tests such as CT scan, colorectal endoscopy, fine needle aspiration (FNA) revealed secondary metastatic lesion of HCC in sigmoid colon. This is the first gastrointestinal (GI) tract metastatic we have encountered. CONCLUSION HCC metastases of the colon are rare, especially cases of hematogenous spread. The prognosis of these patients is often very critical. Indications for surgical removal of the lesion may be used if the general situation of patient is acceptable. Copyright © 2019 Binh Van Pham, Huynh Huu Phan, Lam Le Ngo, Hang Thi Thuy Nguyen, Ky Van Le, Thien Chu Dinh, Nguyen Duy Bac.