Boyerknowles7142

Electric field-assisted aerobic composting (EAC) has been considered as a novel and effective process for enhancing compost maturation. However, the poor conductivity of compost piles affects the efficiency and applicability of EAC. Thus, this study aims to examine how conductive biochar affects compost maturation in biochar-added electric field-assisted aerobic composting (b-EAC). Our results demonstrated that the germination index and humus index significantly increased, and the compost maturation time was shortened by nearly 25% during b-EAC compared to EAC. The total oxygen utilization rate and total relative abundance of electroactive bacteria during b-EAC increased by approximately two and three times those in EAC, respectively. These findings indicated that the addition of conductive biochar has a synergistic effect which facilitated oxygen utilization by reducing resistance and accelerating electron transfer. Therefore, the addition of conductive biochar is proved to be an effective and applicable strategy for optimizing the efficiency of EAC.Sponge iron is a potential material for nitrogen and phosphate removal. To explore the performances and mechanisms of nitrogen and phosphate removal by sponge iron, a sponge iron biofilter was established. The results showed that nitrate was completely removed at HRT of 48 h without external carbon source and at HRT of 3 h with C/N ratio of 5. Furthermore, it was easy to achieve partial denitrification at HRT of 1 h with C/N ratio of 3. The mechanisms of nitrate removal were chemical reduction and nitrate dependent ferrous oxidation without external carbon source and heterotrophic denitrification with external carbon source. XPS result indicated that phosphate was removed by the formation of ferric phosphate precipitation. High throughput sequencing showed that iron-oxidizing bacteria Gallionellaceae was highly enriched in biofilter, accounting for 17.83%, which indicated that it was feasible to achieve autotrophic nitrate removal by sponge iron biofilter.The slaughterhouse waste (SHW) contains high organics which makes SHW a feasible feedstock for anaerobic digestion (AD). The present study systematically assessed the microbiome response and biomethanation along with the production of volatile fatty acids (VFAs) and ammonia under 2%, 4%, 6%, and 8% (w v-1) loadings of SHW in AD. The optimum loading was 2% SHW which resulted in maximum biomethane production and VFAs consumption. A higher SHW concentration (4% and 6%) resulted in a prolonged lag-phase and decreased biomethane production. High VFAs (28.88 g L-1) and ammonia nitrogen (>4 g L-1) accumulation were observed at 8% SHW leading to permanent inhibition of biomethane and methanogenic archaea. An increase in ammonia and VFAs concentration, at 4% and 6% SHW loadings, shifted the methanogenic pathway from acetoclastic to hydrogenotrophic lead by Methanoculleus. Acetoclastic Methanosaeta (77.15%) dominated the reactors loaded with 2% SHW resulting in the highest biomethane production.The role of different biochars in Cr(VI) removal process is not clear. Two raw materials with distinct components were prepared into biochars at various pyrolysis temperatures. The biochar derived from cotton stark (with less lignin than walnut shell) always had more surface functional groups. The pyrolysis temperature had a significant effect on the biochar. In the sorption experiments, the fitting results of two compartment models showed that slow compartment dominated by surface functional groups plays a leading role in the Cr(VI) removal by low-temperature char, while the contribution of the rapid or slow compartment to the process of high-temperature char was equal. In the experiment of biochar mediated the lactate to reduce Cr(VI), the biochar derived from walnut shell (richer lignin) had better conductivity due to its highly aromatic structure, which could directly transfer electrons. This study indicated that various biochars may play different roles in the Cr(VI) removal process.To recover the biogas from sewage sludge and paper waste (PW), the methanogenic performance of thermophilic anaerobic co-digestion of sewage sludge with PW was assessed by a continuous experiment. The effects on the biogas production and microbial community were investigated by changing the PW ratio from 0 to 66.7%. The optimal performance was obtained at the ratio of sewage sludge PW = 46 (total solids), where the COD removal efficiency and biogas production increased from 58.34±5.90% to 72.92±0.08% and 438±53 to 594±72 mL/g-VSadded, respectively. By investigating the trend of carbohydrate and protein degradation rates, the competition between carbohydrate and protein degradation was quantified. The critical PW addition ratio was about (63.64%), where the protein degradation rate decreased to zero with increasing PW addition. Meanwhile, the microbial analysis showed that cellulolytic bacteria outcompeted proteolytic bacteria and to be the predominant group after PW addition.Long noncoding RNAs (lncRNAs) are noncoding RNAs more than 200 nucleotides in length. A growing number of reports indicate that lncRNAs play a key role in multiple cancers by serving as oncogenes or tumor suppressor genes. MAGI2 antisense RNA 3 (MAGI2-AS3) is ubiquitously expressed in human cancers, and the level of MAGI2-AS3 expression is associated with the progression and prognosis of cancers. Moreover, dysregulation of MAGI2-AS3 has been found to regulate cancer cell proliferation, cell death, invasion and metastasis and treatment resistance by serving as a competing endogenous RNA (ceRNA), epigenomic regulator, and transcriptional regulator. Moreover, increasing evidence shows that MAGI2-AS3 may be a potential biomarker for cancer prognosis and a potential target for cancer therapy. In this review, we summarize current research on the functions, mechanisms and clinical significance of the lncRNA MAGI2-AS3 in cancer development.Obesity is increasing rapidly across the globe. It is widely accepted that natural products with a long safety background may modulate obesity. The current work aimed to investigate the effect of Nigella sativa, atorvastatin, or L-Carnitine on high-fat diet-induced obesity in white male albino rats. A regular basal diet was fed to 7 rats, and a high-fat diet (HFD) was fed to 24 rats throughout the study for 12 weeks. The HFD group was split equally into four subgroups, each containing six rats. The first group fed on HFD with no medication, the second group received HFD+ Nigella sativa, the third group received HFD+ atorvastatin, and the fourth group received HFD+L-carnitine. At the beginning of the seventh week (the start of the treatment regimen), Nigella sativa, atorvastatin, or L-Carnitine were administered for six weeks. Glucose, body weight, serum atherogenic index (AI), ALT, and AST activities were analyzed. The pathological alterations in the hepatic tissues were examined microscopically and scored. The results revealed that the HFD diet significantly increased the final body weight, serum AI, and serum levels of liver enzymes. Treatment with L-carnitine or Nigella sativa significantly normalized the lipid profile and decreased the final body weight, serum AI, and Serum ALT. Histopathological examination of the liver of HFD received rats showed features of steatosis, which were mitigated by the administration of Nigella sativa or L-Carnitine, while atorvastatin had no significant effect on the improvement of hepatic lesions. Collectively, study findings showed that Nigella sativa or L-Carnitine has mitigated effects on metabolic and histopathological changes in the liver tissues of rats fed with HFD.Xijiao Dihuang decoction combined with Yinqiao powder (XDD-YQP) is a classical combination formula; however, its therapeutic effects in treating influenza viral pneumonia and the pharmacological mechanisms remain unclear. The therapeutic effect of XDD-YQP in influenza viral pneumonia was evaluated in mice. Subsequently, an everted gut sac model coupled with UPLC/Q-TOF MS were used to screen and identify the active compounds of XDD-YQP. Furthermore, network pharmacological analysis was adopted to probe the mechanisms of the active compounds. Lastly, we verified the targets predicted from network pharmacological analysis by differential bioinformatics analysis. Animal experiments showed that XDD-YQP has a therapeutic effect on influenza viral pneumonia. Moreover, 113 active compounds were identified from intestinal absorbed solutions of XDD-YQP. Using network pharmacological analysis, 90 major targets were selected as critical in the treatment of influenza viral pneumonia through 12 relevant pathways. Importantly, the MAPK signaling pathway was found to be closely associated with the other 11 pathways. Moreover, seven key targets, EGFR, FOS, MAPK1, MAP2K1, HRAS, NRAS, and RELA, which are common targets in the MAPK signaling pathway, were investigated. These seven key targets were identified as differentially expressed genes (DEGs) between influenza virus-infected and uninfected individuals. Hence, the seven key targets in the MAPK signaling pathway may play a vital role in the treatment of influenza viral pneumonia with XDD-YQP. This research may offer an integrative pharmacology strategy to clarify the pharmacological mechanisms of traditional Chinese medicines. The results provide a theoretical basis for a broader clinical application of XDD-YQP.Foetal alcohol spectrum disorder (FASD) is the umbrella term used to describe the physical and mental disabilities induced by alcohol exposure during development. Early alcohol exposure induces cognitive impairments resulting from damage to the central nervous system (CNS). The neuroinflammatory response accompanied by neurodegenerative mechanisms contribute to those detrimental alterations. Cannabidiol (CBD) has recently emerged as an anti-inflammatory drug that might be useful to treat several neuropsychiatric disorders. In our study, we assessed the effects of CBD on long-lasting cognitive deficits induced by early alcohol exposure. Furthermore, we analysed long-term pro-inflammatory and apoptotic markers within the prefrontal cortex and hippocampus. To model alcohol binge drinking during gestational and lactation periods, we used pregnant C57BL/6 female mice with time-limited access to 20% v/v alcohol solution. Following the prenatal and lactation alcohol exposure (PLAE), we treated the male and female offspring with CBD from post-natal day (PD) 25 until PD34, and we evaluated their cognitive performance at PD60. TG003 Our results showed that CBD treatment during peri-adolescence period ameliorates cognitive deficits observed in our FASD-like mouse model, without sex differences. Moreover, CBD restores the PLAE-induced increased levels of TNFα and IL-6 in the hippocampus. Thus, our study provides new insights for CBD as a therapeutic agent to counteract cognitive impairments and neuroinflammation caused by early alcohol exposure.Transplantation of olfactory ensheathing cells (OECs) is a promising approach for repairing the injured nervous system that has been extensively trialed for nervous system repair. However, the method still needs improvement and optimization. One avenue of improving outcomes is to stimulate OEC migration into the injury site. Liraglutide is a glucagon-like peptide-1 receptor agonist used for management of diabetes and obesity. It has been shown to be neuroprotective and to promote cell migration, but whether it can stimulate glial cells remains unknown. In the current study, we investigated the effects of liraglutide on OEC migration and explored the involved mechanisms. We showed that liraglutide at low concentration (100 nM) overall promoted OEC migration over time. Liraglutide modulated the migratory behavior of OECs by reducing time in arrest, and promoted random rather than straight migration. Liraglutide also induced a morphological change of primary OECs towards a bipolar shape consistent with improved migration.