Boyeodom1694

Environmental surveillance as a part of wastewater-based epidemiology (WBE) of SARS-CoV-2 can provide an early, cost-effective, unbiased community-level indicator of circulating COVID-19 in a population. The objective of this study was to determine how widely SARS-CoV-2 detection in wastewater is being investigated and what methods are used. A survey was developed and distributed, with results showing that methods were rapidly applied to conduct SARS-CoV-2 WBE, primarily to test wastewater influent from large urban wastewater treatment plants. Additionally, most methods utilized small wastewater volumes and the primary concentration methods used were polyethylene glycol precipitation, membrane filtration and centrifugal ultrafiltration followed by nucleic acid extraction and assay for primarily nucleocapsid gene targets (N1, N2, and/or N3). Since this survey was performed, many laboratories have continued to optimize and implement a variety of methods for SARS-CoV-2 WBE. Method comparison studies completed since this survey was conducted will assist in developing WBE as a supplemental tool to support public health and policy decision making responses.Restoration has been increasingly adopted to halt trends in coastal wetland loss globally. Existing restoration often assumes that once abiotic stress is relieved, disturbances are prevented, and invasive species are eradicated, coastal wetlands will recover if propagules of native species are supplied either through natural dispersal or planting. Whether other factors including consumers can help explain the often suboptimal performance of existing restoration remains poorly understood. In a series of field experiments in the Yangtze estuary, we examined the relative importance of abiotic stress and crab grazing in regulating the recovery of the native foundation plant species Scirpus mariqueter in salt marsh areas where exotic cordgrass was successfully eradicated. We found that grazing by herbivorous crabs, rather than abiotic stress, was the primary obstacle restricting the recovery of planted Scirpus. This negative effect of crab grazing varied predictably across elevation and was strongest at low elevations where abiotic conditions were positive for Scirpus. These findings highlight that i) measures to control crab grazing are needed to enhance the success of Scirpus restoration, even in areas where abiotic conditions are set to be optimal, and ii) restoration measures purely focused on reducing abiotic stress could be ineffective or suboptimal in field conditions, likely jeopardizing restoration investment and success. Since top-down control of foundation plant species is common in many coastal wetlands and can be especially important in degraded systems where herbivores are abundant, we urge that future coastal wetland restoration assesses for the impacts of grazers and, when present, apply intervention measures.Salinity stress is common for plants growing in coastal wetlands. The addition of biochar in the soil may alleviate the negative effect of salinity through its unique physicochemical properties. To test this, we conducted a greenhouse experiment where the cosmopolitan wetland plant Phragmites australis was subjected to four salinity treatments (0, 5, 10 and 15‰) and three biochar treatments (no biochar addition, with biochar addition and with biochar-compost addition, both biochar and compost were made from P. australis) in a factorial design. Both biochar addition and biochar-compost addition to the substrate enhanced belowground mass of P. australis, application of biochar-compost significantly increased total mass by 35.5% and net photosynthesis rate of P. australis by 51.4%. Both biochar addition and biochar-compost addition significantly increased soil organic carbon content by 62.9% and 31.7%, respectively, but decreased soil ammonium nitrogen content. In the saline soil, application of the mixture of biochar-compost had a strong, and positive effect on the growth of P. australis, compared to biochar alone. Therefore, incorporation of biochar and compost might be an appropriate approach to improve the productivity of P. australis growing in coastal wetlands, where soil salinity is a common environmental stress.An ex situ fermentation system (EFS) can efficiently transform and utilize nitrogen in swine wastewater and reduce environmental pollution. High-throughput sequencing was used to study the relationship between the succession of total bacteria, fungi, and functional bacteria in a swine wastewater EFS, as well as nitrogen metabolism and environmental factors. During the fermentation process, inorganic nitrogen gradually accumulated and the pH changed rapidly from weakly acidic to alkaline. The dominant genera of bacteria, fungi and functional bacteria carrying amoA, nirK, and nosZ genes changed gradually, and Clostridium sensu stricto 1, Thermomyces, Nitrosomonas, Mesorhizobium, and Pseudomonas genera became the most abundant, which showed positive correlations with temperature, pH, and nitrogen levels. Other changed populations showed different correlations with environmental factors, and physical-chemical factors explained more variation of microorganisms than nitrogen resources. These findings contribute to a comprehensive understanding of nitrogen metabolism in EFSs from a molecular micro-ecology perspective.Unrestricted cattle access to streams and rivers can be a significant source of pollution in fluvial systems, contributing to bank erosion and fine sediment inputs. Despite this pressure, observational data are scarce. This study quantified stream bank geomorphic modifications caused by cattle access at fine scale using motion-capture cameras and Terrestrial Laser Scanning (TLS) campaigns. Continuous monitoring of rainfall, discharge, conductivity and turbidity further augmented this dataset. The application of these techniques extended over a five-month grazing period in agricultural sub-catchments with intensive cattle production. At low flow, high-resolution water quality data showed that the frequency of cattle activity in and around stream margins was associated with elevated turbidity signals downstream. However, when elevated turbidity coincided with high flow events, it was not possible to distinguish between local erosion and upstream sediment transfers. Plinabulin TLS results indicated a loss of 0.141 m3 to 1.