Borretodd9682

In this study, polyacrylonitrile (PAN) nanofiber membrane was prepared by an electrospinning technique. After alkaline hydrolysis, the ion-exchange nanofiber membrane (P-COOH) was grafted with chitosan molecules to form a chitosan-modified nanofiber membrane (P-COOH-CS). Poly(hexamethylene biguanide) (PHMB) was then covalently immobilized on P-COOH and P-COOH-CS to form P-COOH-PHMB and P-COOH-CS-PHMB, respectively. The nanofiber membranes were subjected to various surface analyses as well as to the evaluations of antibacterial activity against Escherichia coli. The optimal modification conditions for P-COOH-CS-PHMB were attained by water-soluble chitosan at 50 kDa of molecular weight, coupling pH at 7, and 0.05% (w/w) of PHMB. Within 10 min of treatment, the antibacterial rate was close to 100%. Under the similar conditions of antibacterial treatment, the P-COOH-CS-PHMB exhibited a better antibacterial efficacy than the P-COOH-PHMB. When the number of bacterial cells was increased by 2000 folds, both types of nanofiber membranes still maintained the antibacterial rate close to 100%. After five cycles of repeated antibacterial treatment, the antibacterial efficacy of P-COOH-PHMB was 96%, which was higher than that of P-COOH-CS-PHMB (83%). The experimental results revealed that the PHMB-modified nanofiber membranes can be suitably applied in water treatment such as water disinfection and biofouling control. Wide sustainability and reusability of biomacromolecules such as carbohydrates and proteins-based biopolymers pave the way for providing maximal importance in the field of generating biopolymeric nanoparticles. As compared to synthetic nanomaterials, carbohydrate and protein based biopolymeric nanomaterials offer unique advantages that include antibacterial, biocompatible, immunogenicity, and biodegradable properties. Additionally, they have the significant property of more size distribution. Carbohydrate nanoparticles are primarily derived from the polysaccharide biopolymers such as alginate and chitosan; and protein nanoparticles are made from the diverse peptide biopolymers such as albumin, keratin, sericin, fibroin, gelatin and collagen. Advanced methods such as emulsification, desolvation, electrohydrodynamic atomization and coacervation are employed for the controlled fabrication of green biomacromolecules based nanoparticles. Suitability of biopolymeric nanoparticles in plethora of biotechnological applications are quite feasible with the advent of advanced technologies such as dynamic light scattering, scanning electron microscopy, transmission electron microscopy, atomic force microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and UV visible spectroscopy etc. Applications of such biomacromolecules nanoparticles are highly prevalent in agriculture, food, and biomedical industries. Thus, contributions of biopolymeric nanoparticles derived from carbohydrates and proteins biomacromolecules and their recent trends of patents granted in the biotechnological applications are critically discussed along with a promising future scope. This paper explores the application of cashew gum (CG) as an in vitro antiproliferative, firstly by isolating and characterizing the gum using elemental analysis, gel-permeation chromatography, nuclear magnetic resonance (NMR) and atomic force microscopy (AFM). The molar mass of isolated CG was in the order of 103-104 g/mol, with small protein traces present. Polymer characterization by NMR identified key signals correlating to galactose, glucose, rhamnose and acid-related groups. Three distinct conformational stages were observed by AFM. The impact of CG on cell morphology and viability with both tumor and non-tumor cell lines was studied by AFM and 3-(4,5-dimethyl-2-thiazole)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay respectively. Antiproliferative activity was confirmed for HCT116 (colorectal carcinoma), B16F10 (melanoma) and HL60 (promyelocytic leukemia) cancer cell lines. A change in cell morphology was demonstrated as an increased surface roughness for HL60. Considering that a CG does not exhibit cytotoxicity to non-tumor lines, it can be seen that the CG shows selectivity for tumor cells and can be a promising biomaterial for future studies. V.Mung bean seeds were germinated at 25 °C for 12, 24, 36, 48, 60 and 72 h. Changes in structural and physicochemical properties of mung bean starch during germination were investigated. Microscopy analysis showed germination did not change the shape of starch granules, but granules isolated from germinated mung bean starch exhibited dents surface structure compared with smooth surface of native starch. The molecular weight of starch isolated from germinated mung bean was found to be smaller than that of native ones. Although germinated mung bean showed greater crystallinity than that of native sample, the germination did not change the crystalline structure type of starch (C-type). Moreover, solubility and swelling power of starch varied depending on germination duration and testing temperature. Peak viscosity and trough viscosity slightly decreased as germination prolonged to 72 h. Furthermore, starch isolated from mung bean germinated for 12 h showed greater enthalpy (ΔH) than that of starch isolated from native sample; while, ΔH decrease as germination time prolonged from 36 to 72 h. Benzylpenicillin potassium in vitro The obtained results may help in understanding the influence of germination on functional properties of mung bean starch and choosing appropriate applications to promote utilization of mung bean starch in food industry. In this research paper, the utilization of the magnetic calcium alginate/carboxymethyl chitosan/Ni0.2Zn0.2Fe2.6O4 (CA/CMC/Ni0.2Zn0.2Fe2.6O4) was investigated for the simultaneous aqueous adsorption of Nd (III), Tb (III), and Dy (III). The magnetic products were characterized by FE-SEM, EDX, XRD, FT-IR, TGA, and VSM techniques. The saturation magnetization value for Ni0.2Zn0.2Fe2.6O4 and CA/CMC/Ni0.2Zn0.2Fe2.6O4 was found to be 45.87 and 14.14 emu/g, respectively. Using RSM, a quadratic polynomial equation was obtained to predict the adsorption efficiency of each ion. Under the conditions of pH = 5.5, adsorbent dosage of 0.1 g, initial concentration of 30 mg/L, and contact time of 53 min predicted by RSM, the adsorption efficiencies of Nd (III), Tb (III), and Dy (III) were respectively 95.72, 96.17, and 99.44%. The isotherm and kinetic data were respectively fitted well with Freundlich and pseudo-second-order (PSO) models. The desorption of the loaded ions was effectively carried out by 0.2 M HNO3, and the adsorbent was consecutively utilized with 2.