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Based on the results, the mean score of quality of life before the intervention was 51.73 ± 13.91, and after the intervention was 60.46 ± 13.80 (P less then 0.001). Also, in all four dimensions of quality of life, the mean score of individuals after the intervention increased compared to before the intervention, but this difference was recognized as significant in only two dimensions of physical and mental health (P less then 0.001). The results of HE4 gene expression also showed that supportive and educational care of nurses had a significant effect on the expression of this gene. Therefore, this study confirmed the positive effect of educational and supportive programs in improving the quality of life of patients with ovarian cancer. In general, the design and implementation of such programs are proposed more widely and based on patients' educational and supportive needs.Stroke is the most common, deadly, and complicating neurological disease. Many studies have shown that the levels of some acute inflammatory reactants in people with ischemic stroke are higher than average. Therefore, in this study, three acute inflammatory reactants, i.e., C-reactive protein, Serum cystatin C, and carbohydrate antigen 125, were evaluated in patients with acute ischemic stroke to consider the association between these serums with intra and extra-cerebral vessels stenosis. In this cross-sectional study, 90 patients with non-embolic ischemic stroke were evaluated. The diagnosis was by physical examination, rejection of emboli, and brain imaging. Blood samples were taken in the first 24 hours of a stroke. ELISA test was used to measure CRP, Serum cystatin C, and CA125. Doppler ultrasound of cerebral arteries was also performed in the first five days. Independent chi-square and t-tests were used to analyze the data. The result of CRP level in patients with stenosis was 7.58±1.33μg/ml and in patieof the internal carotid artery and middle cerebral artery is more common in patients with ischemic stroke with high serum CRP levels. This finding suggests that abnormal CRP may be more associated with narrowing some cerebral arteries.with the advent of a large number of drugs in recent years and the aggravation of human aging, drug-induced liver injury is increasing year by year. The protective effect of dandelion extract on acetaminophen (APAP) - induced drug-induced liver injury in rats and its specific mechanism was studied by in vitro cell culture. For this aim,twenty healthy SD rats with the same physiological status were divided into model group and normal group, with 10 rats in each group. The drug-induced liver injury model was made by intragastric administration of 1 g/kg APAP for 14 days. The liver function lactate dehydrogenase (LDH), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were detected to verify the success of the model. After that, the liver tissues were aseptically isolated from the normal group and APAP model group, and the primary hepatocytes were cultured. They were divided into control group (control), liver injury model group (model), medium-dose dandelion extract group (1mm dlwe) and high d liver injury model group had more suspended cells, pseudopodia, polygonal and poor growth state. The cells in the medium-dose dandelion extract group (1mm dlwe) and high dose dandelion extract group (2mm dlwe) adhered well, mostly oval, similar to the normal group and grew well. CCK8 found that the cells in the model group decreased significantly, the proliferation activity decreased significantly, the ast, alt, LDH and ROS in the cell supernatant of the model group increased compared with other groups (P less then 0.05), and the contents of GSH and GSH PX decreased (P less then 0.05). Apoptotic cells in the model group increased, and TNF in the model group-α, COX-2, CYP2E1, MAPK, JNK and NF KB p65 increased (P less then 0.05). CYP2E1, MAPK and NF KB p65 increased in the model group (P less then 0.05).Stroke is the leading cause of neurological problems and the third leading cause of death globally, leading to various neurological defects. Due to the importance of applying nursing and rehabilitation measures to reduce complications in these patients, a study was conducted to determine the effect of nursing and rehabilitation measures on the quality of life of patients with stroke. This two-stage experimental study (before and after) was performed on 20 patients with stroke admitted to the internal medicine department. Patients were selected by sampling method, which had inclusion criteria. Data were collected using a questionnaire based on the quality of life in four areas of physical, mental, general health, and social functioning before and after the intervention. Real-Time PCR measured the expression of Bcl2 / Bax genes. Descriptive and inferential statistics analyzed the data. The results showed that the mean quality of life scores in physical function, psychological, social position, and general health after nursing and rehabilitation measures increased significantly (p = .05). Also, the quality of life score after these measures had a significant increase compared to before (p = .05). Also, a significant increase was observed in the expression ratio of the Bcl2 / Bax genes in the study group compared to the control group, which indicates the effect of nursing and rehabilitation measures on cerebral ischemia. The findings showed that the application of nursing and rehabilitation measures positively affects various aspects of patients' quality of life with stroke. These programs should be provided while educating patients and their families to help them achieve greater independence in the future.Periodontal ligament fibroblasts (PDLFs) play a vital role in the period of periodontal regeneration. In addition, studies show that diphenylhydantoin (phenytoin) increases the growth of gingival fibroblasts. If this effect is also present in the periodontal ligament (PDL) fibroblasts, it may be used to regenerate periodontal tissues. Accordingly, this study aimed to compare the effect of phenytoin on the growth rate of gingival fibroblast cells and PDL in the cell culture medium. In this regard, 10 Wistar rats were selected. The gingival specimen was obtained from the area between the upper teeth, and the PDL specimen was obtained from the middle third of the lower teeth root. After transferring the samples to a suitable culture medium for culturing PDL and gingival fibroblasts, each sample was divided into two experimental and control groups. In the experimental group, 20 mg/ml phenytoin dissolved in sodium hydroxide was added to Dulbecco's modified Eagle's medium (DMEM). After 48 hours, fibroblast cell proliferation was assessed through a 1-WST cell proliferation kit by ELISA. The proliferation of gingival fibroblast cells and PDL in both test and control groups were statistically analyzed by the independent t-test. The results showed that the effect of phenytoin on the proliferation of gingival fibroblast cells and PDL fibroblast cells is significant. Also, the proliferation of PDL cells was significantly different from gingival cells in the experimental group (P less then 0.001) and was higher in PDL cells. check details In general, in this study, it was found that phenytoin in vitro, like in vivo, is able to increase the proliferation of gingival fibroblast cells, and this phenytoin effect is also present in PDL fibroblast cells.Nowadays, the incidence and mortality of head and neck tumors are gradually increasing. Head and neck malignant tumors (such as laryngeal cancer, hypopharyngeal cancer, oral cancer, nasopharyngeal cancer, oropharyngeal cancer, and other head and neck malignancies) are more common among systemic tumors. The most common pathological head and neck tumor type is squamous cell carcinoma, accounting for about 90%. In this study, immunohistochemical methods were used to collect the normal squamous epithelial tissues of the head and neck, atypical hyperplasia tissues, and head and neck squamous cell carcinomas on a tissue chip for detection. The recombinant LATS1 overexpression plasmid was prepared and transferred into B88 cells. Western blotting, MTT, and Transwell chamber methods were used to detect the effects of LATS1 proliferation, migration, and B88 cell overexpression. The experimental results showed that in head and neck squamous cell carcinoma, the expression of LATS1 protein decreased from 59.3% to 11.3%. At the same time, this protein inhibited the proliferation, migration, and invasion of head and neck squamous epithelial cells and also inhibited epithelium- Interstitial transformation exerts its tumor suppressor effect, indicating that LATS1 may play a tumor suppressor effect as a tumor suppressor gene. An in-depth study of the role and mechanism of LATS1 protein in the occurrence of head and neck squamous cell carcinoma may provide new opportunities for the treatment of head and neck squamous cell carcinoma in the future.CTLA4 is a regulator gene for T cells and relates to autoimmune diseases. By using a case-control method, CTLA4 functional single-nucleotide polymorphisms for potential associations with Type 1 diabetes mellitus in an Iraqi children's population. ARMS-PCR method is used for genotyping +49AG (rs231775) variations in 60 obese children and 60 ethnically matched controls; all measured subjects were (fasting glucose, fasting insulin, and HbA1c). The glucose oxidase method is used to determine plasma glucose levels. The amounts of insulin in the blood were determined using a radioimmunoassay (RIA); Insulin resistance was measured using the HOMA-IR index. A HOMA-IR cut-off level of 2.5 was acceptable. There was no significant difference in allele and genotype frequencies between the groups, according to CTLA4 +49AG analyses. In conclusion, AA cases had a high frequency of A/A genotype than healthy participants but lower rates of A/G and G/G genotypes.Although the production of the secondary metabolite is frequently restricted, methods to regulate and optimize their synthesis are extremely beneficial. The current study proposes to enhance the production of antibiofilm metabolite in Streptomyces cellulosae (S. cellulosae). It was isolated from soil by growing on Gause's media and identified by colony morphology and genomic sequencing of 16S rDNA. Antibacterial and antibiofilm activities of the isolates were screened against a series of pathogenic bacteria by agar plug diffusion and 96 well microtiter plate methods, respectively. Physiological regulation of the bacterial bioactivity against biofilm formation was monitored under different cultural conditions. The isolated Streptomyces sequence analysis of the 16S rDNA was 100% identical to the sequence of S. cellulosae strain NBRC 13027. Physical (temperature and pH) and chemical (carbon, nitrogen, and minerals) culture medium factors have shown variable impacts on the growth and bioactive substances of S. cellulosae. Moreover, results of simple linear regression and correlation suggested that most of the physiological regulations with the highest response (r2= 0.85-0.99; p less then 0.01) and linearly (r= 0.88-0.99; p less then 0.01) were correlated between microbial biomass and crude extract. Lastly, under different culture growth conditions, biofilm inhibition was tested against Pseudomonas aeruginosa (P. aeruginosa). The physiological regulation results exhibited that 1 μg/mL of the extract was the most efficient concentration against biofilm formation in P. aeruginosa while 3 μg/mL is an effective bactericidal dose against P. aeruginosa. We concluded that S. cellulosae can produce antibacterial and antibiofilm metabolites. Physiological regulation is considered a powerful tool that can be used for increasing the biosynthesis of the active metabolites and biomass.