Blevinsdinesen9679

Mitochondrial transcription factor A (TFAM), which is required for mitochondrial DNA (mtDNA) transcription, has been linked to metabolic changes that contribute to tumorigenesis and chemoresistance. In this work, we investigated the expression pattern and role of TFAM in hepatocellular carcinoma (HCC). TFAM expression level is similar in 18 out of 20 paired normal liver and HCC tissues with only 2 HCC tissues showing 1.8-fold increase in TFAM. Similar phenomenon was observed in HCC cell lines compared to normal liver lines. Interestingly, TFAM expression is upregulated in resistant HCC cells regardless of the differential TFAM expression level in their parental lines and mechanism of resistance. TFAM depletion led to inhibition of growth and survival but not migration, and sensitization to doxorubicin and sorafenib treatment, through AMPK activation, reduction of nucleoside triphosphates and mitochondrial respiration in HCC cells. In addition, we demonstrated that resistant HCC cell lines were more sensitive to TFAM inhibition than parental lines, and this might be due to the increased mitochondrial biogenesis in resistant HCC cell lines. Our work reveals the preferential role of TFAM in HCC cell response to standard of care drugs, which suggests a potential sensitizing therapeutic target for HCC treatment.Background/objective The transanal total mesorectal excision(TaTME) of rectal malignancies is largely referred to as treatment of mid to low, especially low rectal cancer. This study was to compare the short-term efficacy of TaTME and laparoscopic total mesorectal excision (LaTME) for low rectal cancer. Methods A prospective study of patients with low rectal cancer who underwent laparoscopic radical surgery at the General Surgery of Guangzhou Red Cross Hospital from January 2017 to December 2019 was performed. The general information, perioperative results and pathological results of the two groups were compared. Results A total of 64 patients were included in the study, 32 in the TaTME group and 32 in the LaTME group. The clinical characteristics of the two groups was comparable (P > 0.05). The operation time in the TaTME group was longer than that in the LaTME group (212.59 ± 28.71min vs 187.66 ± 27.15min, P = 0.001), no significant differences were seen in the conversion rate, intraoperative complications, morbidity, serious morbidity, anastomotic leak, unplanned reoperation and hospital stay(P > 0.05). The circumferential resection margin (CRM) distance in the TaTME group was longer than that in the LaTME group (6.81 ± 2.99 mm vs 5.21 ± 3.06 mm, P = 0.039). The inter-group difference in terms of harvested lymph nodes, mesorectum integrity, CRM involvement, DRM distance, R1 resection, complete remission, pathological T stage, pathological N stage and pathological TNM stage was not significant (P > 0.05). Conclusions TaTME is a promising surgical technique and maybe offers a safe and feasible alternative to LaTME in managing low rectal cancer.Purpose The purpose of this study was to examine the effects of school-based meditation courses on middle school students' self-reflection, academic attention (ability to focus in classrooms), and subjective well-being. Design and methods The research design was a nonequivalent group comparison (n = 163) with pretest and post-test. The experimental group (n = 81) was given an eight-week meditation course and the control group (n = 82) was given other elective courses such as calligraphy and reading. Results The experimental group showed significant increases in self-reflection (t = 2.536, p = .012) and academic attention (t = 2.767, p = .006), but subjective well-being did not increase significantly (t = 0.906, p = .367). Life satisfaction was the only subcomponent of subjective well-being that increased significantly (t = 2.438, p = .016); the other subjective well-being subcomponents did not show any significant changes. Conclusions Self-reflection and academic attention significantly increased in middle school students after an eight-week meditation course. Even though changes in subjective well-being were not significant, one of its subcomponents (life satisfaction) did show significant improvement. Self-reflection and subjective well-being were shown to be influential factors for academic attention (48.5% of the variance explained). Practice implications This study is meaningful in that it examined positive benefits of a meditation course in middle school students and explored the feasibility of such a course in a school system.Purpose To determine the purpose of the parents of infants with cleft lip and/or palate (CL/P) for using Facebook, how they share for the audience and what kind of information they search for. Design and methods The results of the study were analyzed using qualitative and quantitative methodologies. Data were collected for one major social media platform (Facebook) from public accounts dedicated to CL/P. The former involved thematic and word count analysis for online posts on the Facebook platform, while the latter employed descriptive statistics. Results Facebook has a total of 8 group accounts and 7695 users in Turkey. Among the 702 posts analyzed, "requesting information" was found to be the most commonly encountered theme (55.4%). This study, it was determined that especially parents of infants with CL/P need information about surgery. The "announcement" was mentioned in only 3.3% of all posts. find more Our text analysis of 17.134 words mentioned in posts revealed that the most common word was surgery (6.6%). Conclusions The current study provides a comprehensive reference to the role of social media in CL/P. Practice implications In the near future, analysis of patients' and public knowledge and perception of surgery will be an important next step in building greater awareness of its role in the management of CL/P.Study objective Single-payer health care is supported by most Americans, but the effect of single payer on any particular sector of the health care market has not been well explored. We examine the effect of 2 potential single-payer designs, Medicare for All and an alternative including Medicare and Medicaid, on total payments and out-of-pocket spending for treat-and-release emergency care (patients discharged after an emergency department [ED] visit). Methods We used the 2013 to 2016 Medical Expenditure Panel Survey to determine estimates of payments made for ED visits by insurance type, and the 2015 National Hospital Ambulatory Medical Care Survey to estimate the proportion of ED visits covered by each insurance type. Results We found that total payments were predicted to increase from $85.5 billion to $89.0 billion (range $81.3 to $99.8 billion) in the Medicare-only scenario and decrease to $79.4 billion (range $71.6 to $87.2 billion) under Medicare/Medicaid, whereas out-of-pocket costs were predicted to decrease from $116 per visit to $45 with Medicare and to $36 with Medicare/Medicaid.