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to measure rehabilitation success, particularly strength and return to activity. Identified evidence gaps should be addressed in future research.

Making impressions of 2-piece implants is typically associated with the repeated disassembly and reassembly of superstructures and related to soft-tissue trauma. Intraoral scanning of 1-piece zirconia implants is problematic because scan bodies are not readily available. Whether using virtual hybrid casts generated by merging intraoral scan data with the known surface geometry of abutments can solve these difficulties is not clearbecause data on accuracy of the workflow are sparse.

The purpose of this invitro study was to evaluate the accuracy of virtual hybrid casts with respect to the impact of different gingival situations. The workflow was designed to render pointless the use of impression posts and scan bodiesand avoid any displacement of the gingiva.

The mandibular right first molar in a typodont was replaced with a 2-piece titanium implant with a custom abutment and then a 1-piece zirconia implant. Three situations representing different gingival heights covering the abutments were simulated. Twehen the preparation margin was covered. When only half of the abutment was captured, a larger deviation of 22.0 μm (IQR 7.0 μm) was observed. The hybrid cast concept demonstrated superior accuracy compared with protocols using scan body scans (76.0 μm; IQR 27.0 μm) and cast scans (23.0 μm; IQR 15.0 μm).

Digital intraoral scanning and the generation of virtual hybrid casts providehigh accuracy and are suitable for the fabrication of single-implant-supported restorations. The atraumatic procedure avoids tissue manipulation and reduces clinical effort.

Digital intraoral scanning and the generation of virtual hybrid casts provide high accuracy and are suitable for the fabrication of single-implant-supported restorations. The atraumatic procedure avoids tissue manipulation and reduces clinical effort.

Evidence is limited for the impact of clinical adjustments and polishing on the longevity of glazed lithium disilicate restorations.

The purpose of this invitro study was to evaluate the influence of surface finishing on the survival and success rates of lithium disilicate restorations based on fatigue resistance and failure mode.

Lithium disilicate (IPS e.max CAD) maxillary premolar crowns (N=54) were cemented on a dentin analog. The restorations were divided into 3 groups overglaze (OG), abrasion (GA), and abrasion and polishing (AP). The crowns were submitted to cyclic fatigue in 37

C water at 100 N and 2 Hz in 2 lifetimes. Memantine The load was applied to the occlusal surface by using anatomic pistons to simulate a clinical tripod occlusal contact. After cycling, the crowns were examined for failure (cracking, chipping, or catastrophic fractures) under optical and scanning electron microscopy. Cracking was considered either a structural failure (success analysis) or a survival (clinical criteria - survival analysis). Data were analyzed by using the log rank Kaplan-Meier and Holm-Sidak tests (α=.05).

Surface finishing had no influence on the structural integrity of lithium disilicate, with similar success rates (P=.720). The calculated survival rate was higher for AP than that for other groups (P=.028). Cracking was found for GA and AP crowns, mostly initiating from the external surface. Chipping occurred in all experimental groups, and AP crowns did not show catastrophic failures.

Although surface treatments had no influence on the success of lithium disilicate, polishing showed a positive effect on the survival rate of the crowns based on the clinical implications of cracking (no need for replacement).

Although surface treatments had no influence on the success of lithium disilicate, polishing showed a positive effect on the survival rate of the crowns based on the clinical implications of cracking (no need for replacement).

The traditional sintering of zirconia takes an extended time, and accelerated treatments have been developed to reduce treatment time and manufacturing costs. Studies evaluating the effect of sintering time on the mechanical properties of zirconia are lacking.

The purpose of this invitro study was to evaluate the effect of the sintering condition and the low-temperature degradation (LTD) of zirconia on the flexural strength of monolithic zirconia.

Zirconia specimens (LUXEN Enamel) were sintered at 1500 °C, 1530 °C, and 1560 °C for 4, 5, 6, 7, and 12 hours and subjected to LTD (n=10). Control specimens were assessed in the nontreated condition. The 3-point flexural strength was measured by using a universal testing machine. The crystal phases of the specimens were compared and quantitatively analyzed through X-ray diffraction (XRD) analysis, and the crystal grain size was measured by using a field emission scanning electron microscope (FE-SEM). Two-way ANOVA and the independent sample t test were used to phase change from tetragonal to monoclinic.

At all sintering times, the flexural strength of zirconia was over 800 MPa, which is the minimum flexural strength of a 4-unit or longer prosthesis as specified in the International Organization for Standardization (ISO) 6872. With LTD exposure, the increase in the flexural strength of zirconia was associated with the phase change from tetragonal to monoclinic.

Occlusal devices can be either conventionally processed, milled, or printed. However, little is known about the biocompatibility of 3D printing resin materials.

The purpose of this invitro study was to compare the viability and morphology of human gingival fibroblast cells (HFG-1) after cultivation on conventionally processed, milled, and printed occlusal device materials with different surface treatments.

Disks of a conventionally processed (PalaXpress Clear [pP]), milled (Yamahachi PMMA Clear [sY]), and 2 different printed materials (Dental LT Clear Resin [aD]; Freeprint splint [aF]) were prepared. The surfaces of the specimens were finished by using 2 different treatments (unpolished and polished with P1200-grit silicon carbide paper). HGF-1 cells were cultivated on the specimens for 24 hours, and a viability assay was performed by using polystyrene disks as a control (n=9 disks per group). Cell morphology and the topography of the specimens were examined with scanning electron microscopy (n=3 disks polishing, cell behavior was similar to that of the conventionally processed and milled specimens.Acute pericarditis is an inflammatory disease associated with a non-negligible risk of acute complications and future recurrence. However, the exact incidence of pericarditis recurrence in patients with a first uncomplicated clinical course is unknown. We sought to evaluate the incidence and clinical predictors of recurrence after a first episode of acute uncomplicated pericarditis in a large urban hospital in the United States. We conducted a retrospective review, through electronic health records, to complete a database that includes patients admitted with a first episode of acute pericarditis and selected only those with an uncomplicated course (without in-hospital death, large pericardial effusion [>20 mm] or tamponade, constriction, or incessant pericarditis) at the VCU Medical Center (Richmond, Virginia) from 2009 to 2018. A total of 240 patients met acute pericarditis criteria of the 240 patients, 164 patients (68%) had an uncomplicated course (median age [interquartile range] in years 50 [32 to 62], 43% females). The median follow-up time was 186 (19 to 467) days. Pericarditis was idiopathic in 84 patients (51%). Fifteen patients (9%) had at least 1 episode of recurrent pericarditis. Compared with those without recurrence, patients with recurrent pericarditis were younger (37 [25 to 59] vs 51 [34 to 62] years, p = 0.034), had a higher prevalence of subacute/delayed presentation (2 [13%] vs 1 [1%], p = 0.023), and less frequently received colchicine (6 [40%] vs 100 [67%], p = 0.036). At multivariate logistic regression analysis, subacute presentation and younger age remained predictors of recurrence at follow-up. In conclusion, 9% of patients with acute pericarditis experienced a recurrence over a 6-month median follow-up despite an initial uncomplicated course. Younger age and subacute presentation were associated with a significantly increased risk of recurrence.

Antibiotics alter the diversity, structure, and dynamics of host-associated microbial consortia, including via development of antibiotic resistance; however, patterns of recovery from microbial imbalances and methods to mitigate associated negative effects remain poorly understood, particularly outside of human-clinical and model-rodent studies that focus on outcome over process. To improve conceptual understanding of host-microbe symbiosis in more naturalistic contexts, we applied an ecological framework to a non-traditional, strepsirrhine primate model via long-term, multi-faceted study of microbial community structure before, during, and following two experimental manipulations. Specifically, we administered a broad-spectrum antibiotic, either alone or with subsequent fecal transfaunation, to healthy, male ring-tailed lemurs (Lemur catta), then used 16S rRNA and shotgun metagenomic sequencing to longitudinally track the diversity, composition, associations, and resistomes of their gut microbiota both witlemur resistomes. Beyond providing new perspectives on the dynamics that govern host-associated communities, particularly in the Anthropocene era, our holistic study in an endangered species is a first step in addressing the recent, interdisciplinary calls for greater integration of microbiome science into animal care and conservation.

Long-term, integrated study post antibiotic-induced microbial imbalance revealed differential, metric-dependent evidence of recovery, with beneficial effects of fecal transfaunation on recovering community composition, and potentially negative consequences to lemur resistomes. Beyond providing new perspectives on the dynamics that govern host-associated communities, particularly in the Anthropocene era, our holistic study in an endangered species is a first step in addressing the recent, interdisciplinary calls for greater integration of microbiome science into animal care and conservation.

Whole-plant cannabis extracts are consumed by the public for medical and non-medical ("recreational") purposes but are poorly researched compared to pure cannabinoids. There is emerging evidence that cannabis extracts comprising complex mixtures of cannabinoids may have different biological effects from that of pure cannabinoids. In the current study, we sought to assess the effect of whole-plant cannabis extracts produced from different chemotypes of cannabis on the normal behavior of zebrafish larvae.

Three cannabis plant chemotypes were used in this study that contained either high amounts of THC, high amounts of CBD, high but equal amounts of THC and CBD, or low but equal amounts of THC and CBD. Following solvent extraction, liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) was performed for the detection and quantitation of target cannabinoids. Larval zebrafish behavioral models were subsequently used to assess the effect of the four different whole-plant cannabis extracts on the normal larval behavior using the DanioVision behavioral tracking systems and software.