Bengtsendickey0147

KGaA, Weinheim.Esophageal squamous cell carcinoma (ESCC) belongs to one of the most common malignant tumors worldwide and possesses high mortality. Long non-coding RNAs (lncRNAs) have been demonstrated to be essential biological participants in the progression of ESCC. Based on bio-informatics prediction, forkhead box P4 antisense RNA 1 (FOXP4-AS1) and forkhead box P4 (FOXP4) were upregulated in esophageal carcinoma samples and were positively correlated with each other. Present study aimed to explore the function of FOXP4-AS1 and FOXP4 in ESCC cells. Function assays disclosed that knockdown of FOXP4-AS1 or FOXP4 efficiently suppressed cell proliferation and induced cell apoptosis. Moreover, FOXP4-AS1 positively regulated FOXP4 by interacting with insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) to stabilize FOXP4 mRNA. Additionally, FOXP4-AS1 could upregulate the expression of FOXP4 by sponging miR-3184-5p. Finally, we found that YY1 is a transcription factor that can transcriptionally activate both FOXP4-AS1 and FOXP4 in ESCC cells. In a word, YY1-induced upregulation of FOXP4-AS1 and FOXP4 promote the proliferation of ESCC cells This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.The preparation of an all supramolecular multi-chromophoric azaborondipyrromethene ( ABDP ) / zinc tetraphenylporphyrin ( ZnTPP ) / exfoliated graphene ( GR ) nanoensemble is accomplished. The ABDP derivative bears glycol chains for enhancing solubility and a pyridine functionality for allowing coordination with ZnTPP . The ABDP / ZnTPP/GR nanoensemble is characterized with regards to morphology and composition using complementary microscopy imaging, thermogravimetric analysis, Raman and steady state and time resolved absorption and emission spectroscopy. The photophysical and electrochemical assessment of ABDP / ZnTPP/GR as well as the binding properties of the ABDP / ZnTPP complex employed as reference are presented. Energy and electron transfer events were observed in ABDP / ZnTPP upon photoexcitation. However, in the case of ABDP / ZnTPP/GR , the graphene induced aggregation of the chromophores alters their electronic interactions, enhancing the energy/electron transfer process between them. © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.There are few studies on heparan sulfate (HS) in the skin, during aging, when estrogen is suppressed. The enzyme heparanase-1 (HPSE-1), has its 17β-estrogen-regulated expression in pathological conditions such as cancer and chronic inflammatory diseases. HPSE-1 is correlated with the matrix metalloproteinase-9 (MMP-9), an endopeptidase that also undergoes estrogen action. We investigated the distribution of HS, expression HPSE-1 and MMP-9 in the skin of adult rats at different ages and in the age-matched ovariectomized rats to evaluate the influence of low estrogen on the distribution of HS. Thirty female Wistar rats were used. Rats underwent to a sham surgery (ctr, n = 15) or to a bilateral ovariectomy (ovx, n = 15) and were euthanized after 45, 75, and 90 days after ovariectomy. Morphological, morphometric, biochemical, and reverse transcriptase polymerase chain reaction (RT-PCR) methodologies were used. A significant decrease (P  less then  0.001) in total skin thickness was observed in the ctr and ovx animals, being higher in the older animals. The thickness of the epidermis and dermis decreased; however, the proportion in the total skin remained similar comparing ctr and ovx. An increase of HS with increasing age and ovariectomy was observed. The expression of the HPSE-1 and MMP-9 enzymes decreased, being higher in old animals. A correlation between the increase of HS and the decrease of the HPSE-1 was demonstrated in both groups. Overall, these data suggested that estrogen acts in the regulation of the expression of the HPSE-1, not only in pathological states, as already established, but also in aging. © 2020 International Federation for Cell Biology.BACKGROUND Head and neck squamous cell carcinoma (HNSCC) has attracted researchers' attention due to its high incidence around the world. This malignancy occurs in the oral cavity, pharynx, and larynx in most cases. Quite a few lncRNAs have been revealed to regulate the malignant neoplasia of several cancers. Nevertheless, effects of lncRNA LINC00467 in HNSCC have not yet been reported. METHODS The expression of LINC00467, miR-299-5p and ubiquitin specific protease-48 (USP48) in HNSCC cells were quantified by RT-qPCR. The influences of LINC00467 deficiency on HNSCC progression were reflected by CCK-8, colony formation, EdU, wound healing and western blot assays. RIP and luciferase reporter assays were conducted to confirm the interaction among LINC00467, miR-299-5p and USP48. RESULTS LINC00467 was considerably upregulated in HNSCC cells, and absence of LINC00467 suppressed cell growth, cell migration and EMT process in HNSCC. Furthermore, miR-299-5p expression was notably downregulated in HNSCC cells, and miR-299-5p could bind with LINC00467. Moreover, USP48 was conspicuously overexpressed in HNSCC cells and capable of binding with miR-299-5p. LINC00467 could upregulate USP48 expression via sponging miR-299-5p. In the end, rescue assays proved that USP48 overexpression could compensate for suppressive effects on HNSCC progression mediated by LINC00467 deficiency. CONCLUSIONS LINC00467 enhances HNSCC progression by serving as a sponge of miR-299-5p to increase USP48 expression. This article is protected by copyright. All rights reserved.Soft and flexible two-dimensional (2D) systems such as liquid interfaces would have much more potentials in dynamic regulation on nano-macro connected functions. In this short review article, we focus especially on dynamic motional functions at liquid dynamic interfaces as 2D material systems. Several recent examples are selected to be explained for overviewing features and importance of dynamic soft interfaces in a wide range of action systems. The exemplified research systems are mainly classified into three categories (i) control of micro-objects with motional regulations; (ii) control of molecular machines with functions of target discrimination and optical outputs; (iii) control of living cells including molecular machine functions at cell membranes and cell/biomolecular behaviours at liquid interface. Sciences on soft 2D media with motional freedom and their nanoarchitectonics constructions would increase importance in future technology in addition to popular rigid solid 2D materials. © 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.RATIONALE The prevalence of type 2 diabetes mellitus (T2DM) is increasing, but the early diagnosis of T2DM in high risk populations remains challenging using only fasting blood glucose (FBG) or hemoglobin A1c measurements. It is, therefore, important to search for integrated biomarker for early diagnosis by determining metabolites associated with the progressopm of the disease. METHODS We recruited 149 participants (51 T2DM patients, 50 individuals with impaired fasting glucose (IFG) and 48 normal glucose tolerance subjects). Their serum samples were analyzed based on a metabolomics approach using UHPLC/Q-Orbitrap HRMS. The changes in metabolites were profiled and evaluated using univariate and multivariate analysis. Furthermore, a biomarker model was established and the potential biomarkers were evaluated using binary logistic regression analysis and receiver operating characteristic analysis with AUC (area under the curve). Pathway analysis of differential metabolites was performed to reveal the important bpport for novel biomarkers in the diagnosis and treatment of diabetes. This article is protected by copyright. All rights reserved.Aromatase is involved in the biosynthesis of estrogen and thus is a critical target for breast cancer. In this study, to identify new aromatase enzyme inhibitors, seven 3-[4-(5-methyl-1H-benzo[d]imidazol-2-yl)phenyl]-6-(substituted phenyl)-7H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazine derivatives were synthesized. First, a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was performed to determine the inhibitory activity of the synthesized compounds on the MCF-7 cell line. The aromatase inhibitory activity was determined for the active compounds 5b, 5c, 5e, and 5g on the MCF-7 cell line. Compound 5g showed significant aromatase inhibitory activity (IC50  = 0.037 ± 0.001 µM). Interestingly, this compound, which bears a difluoro substituent at positions 2 and 4 of the phenyl ring, displayed the most potent aromatase inhibitory activity without significant cytotoxicity to a normal healthy cell line (NIH3T3). Furthermore, the interactions between the best active compounds and the active site of the enzyme were analyzed through a docking study. The results of this study determined that benzimidazole-triazolothiadiazine derivatives are promising compounds that should be further developed as a novel class of aromatase inhibitors. © 2020 Deutsche Pharmazeutische Gesellschaft.OBJECTIVE Peer mentorship has been shown to be helpful for other mental health conditions, but it has been understudied for patients with eating disorders. The goal of the present study was to evaluate the feasibility and efficacy of peer mentorship for individuals with eating disorders by conducting a randomized controlled trial (RCT). TRIAL DESIGN Parallel three-arm pilot RCT with 111 allocation to peer mentorship, social support mentorship (active comparison intervention), and waiting list. METHOD Sixty outpatients with anorexia nervosa (AN), bulimia nervosa (BN), or binge-eating disorder (BED) were randomly assigned to a condition. Outcome measures, including eating disorder symptoms and general psychopathology, were completed at baseline, mid-, and postintervention. RESULTS Session attendance and acceptability ratings were higher in peer mentorship than social support mentorship. More participants in social support mentorship (39%) dropped out compared to peer mentorship (5%). In intent-to-treat analysis, peer mentorship showed greater reductions in body dissatisfaction and anxiety compared with both control groups. Compared with social support mentorship, peer mentorship had greater reductions in depression. Compared with waiting list, peer mentorship had greater reduction in binge eating days/week in patients with BN/BED and restriction days/week in patients with AN. Peer mentorship did not impact body mass index or reentry into higher level of care. DISCUSSION This pilot RCT provides preliminary evidence that peer mentorship is effective for some cognitive and behavioral symptoms of eating disorders as an adjunct to outpatient treatment. Additional studies are needed to evaluate the efficacy of peer mentorship in absence of treatment. © 2020 Wiley Periodicals, Inc.Osteoporosis, fractures, and other bone diseases or injuries represent serious health problems in modern society. A variety of treatments including drugs, surgeries, physical therapies, etc. have been used to prevent or delay the progression of these diseases/injuries with limited effects. Electromagnetic field (EMF) has been used to non-invasively treat bone diseases, such as fracture and osteoporosis, for many years. However, because a variety of cellular and molecular events can be affected by EMF with various parameters, the precise bioeffects and underlying mechanisms of specific EMF on bone cells are still obscure. Here, we summarize the common therapeutic parameters (frequency and intensity) of major types of EMF used to treat bone cells taken from 32 papers we selected from the PubMed database published in English from 1991 to 2018. Briefly, pulse EMF promotes the proliferation of osteoblasts when its frequency is 7.5-15 Hz or 50-75 Hz and the intensity is 0.40-1.55 mT or 3.8-4 mT. Sinusoidal EMF, with 0.