Beardmccarthy7672

In vitro and in vivo studies are fundamental steps in the characterization of new implantable materials to preliminarily assess their biological response. The present study reports the in vitro and in vivo characterizations of a novel experimental silicate bioactive glass (BG) (47.5B, 47.5SiO2-10Na2O-10K2O-10MgO-20CaO-2.5P2O5 mol.%). Cytocompatibility tests were performed using human mature osteoblasts (U2OS), human mesenchymal stem cells (hMSCs) and human endothelial cells (EA.hy926). The release of the early osteogenic alkaline phosphatase (ALP) marker suggested strong pro-osteogenic properties, as the amount was comparable between hMSCs cultivated onto BG surface and cells cultivated onto polystyrene control. Similarly, real-time PCR revealed that the osteogenic collagen I gene was overexpressed in cells cultivated onto BG surface without biochemical induction. Acute toxicity tests for the determination of the median lethal dose (LD50) allowed classifying the analyzed material as a slightly toxic substance with LD50 = 4522 ± 248 mg/kg. https://www.selleckchem.com/products/torin-1.html A statistically significant difference in bone formation was observed in vivo through comparing the control (untreated) group and the experimental one, proving a clear osteogenic effect induced by the implantation at the defect site. Complete resorption of 47.5B powder was observed after only 3 months in favor of newly formed tissue, thus confirming the high osteostimulatory potential of 47.5B glass.In this study, we prepared new antioxidant active plastic bottle caps by incorporating butylated hydroxyanisole (BHA) or butylated hydroxytoluene (BHT) and 2% (w/w) white masterbatch in high-density polyethylene (HDPE). Fourier-transform infrared (FT-IR) spectrometry revealed that the antioxidants and HDPE were uniformly mixed with noncovalent bonding. In addition, the differential scanning calorimetry (DSC) test revealed that the change in melting point and initial extrapolation temperature of the antioxidant active caps was not significant. Sensory evaluation and removal torque tests validated the suitability of the antioxidant active plastic bottle caps in industrial application. The antioxidant activity increased with a greater concentration of BHA and BHT incorporated in both antioxidant active caps (p less then 0.05) and with more impact on the BHA cap compared to BHT cap in terms of antioxidant activity. Migration experiments for 10 days at 40 °C and 2 h at 70 °C showed that active antioxidants in the plastic bottle cap were more easily released into fatty foods and milk products that are highly sensitive to oxidation, and the migration of BHA and BHT did not exceed the maximum amount specified in (EC) No 1333/2008 ( less then 200 mg/kg). As such, the antioxidant active plastic bottle caps inhibited oxidation, thereby ensuring higher food quality.Versatile acrylate-epoxy hybrid formulations are becoming widespread in photo/thermal dual-processing scenarios, especially in 3D printing applications. Usually, parts are printed in a stereolithography or digital light processing (DLP) 3D printer, after which a thermal treatment would bestow the final material with superior mechanical properties. We report the successful formulation of such a hybrid system, consisting of a commercial 3D printing acrylate resin modified by an epoxy-anhydride mixture. In the final polymeric network, we observed segregation of an epoxy-rich phase as nano-domains, similar to what was observed in a previous work. However, in the current work, we show the effectiveness of a coupling agent added to the formulation to mitigate this segregation for when such phase separation is undesired. The hybrid materials showed significant improvement of Young's modulus over the neat acrylate. Once the flexible, partially-cured material was printed with a minimal number of layers, it could be molded into a complex form and thermally cured. Temporary shapes were readily programmable on this final material, with easy shape recovery under mild temperatures. Inspired by repairable 3D printed materials described recently, we manufactured a large object by printing its two halves, and then joined them covalently at the thermal cure stage with an apparently seamless union.The synthesis and application of nanomaterials as antioxidants and cytotoxic agents has increased in recent years. Biological methods go beyond the chemical and physical synthesis that is expensive and not friendly to the environment. link2 Foodborne pathogens and microorganisms causing candidiasis are responsible of 5-10% hospitalized patients. The nutritional properties of the fruit called pitaya, from the Stenocereus queretaroensis species, have been little explored. link3 Therefore, in this study the phytochemical composition of S. queretaroensis peel was evaluated and silver nanoparticles (AgNPs) were synthesized biologically in an environmentally friendly way by S. queretaroensis peel aqueous extract that contains phytochemicals capable of reducing silver nitrate. The antimicrobial activity of the AgNPs was tested by determining the minimum inhibitory concentration (MIC), minimal bactericidal concentration (MBC) and time-kill kinetics. AgNPs were characterized visually, by UV-visible spectroscopy and TEM. FTIR spectroscopy identified metabolites responsible for the AgNPs formation. AgNPs showed potent antimicrobial activity against gram-negative and gram-positive bacteria, against fungi, and a methicillin-resistant strain of S. aureus. MIC and MBC values were as low as 0.078 and 0.156 μg/mL using AgNPs biosynthesized by S. queretaroensis fruit peel and the time kill assay started a log reduction in CFU/mL at 1 × MIC and 2 × MIC. S. queretaroensis-mediated AgNPs could be the basis for the formulation of biofilms for packaging products or as disinfectants for use on different surfaces.Two nanocrystalline ferromagnetic alloys of the Fe-Co-Nb-B system have been produced by mechanical alloying (MA). Their microstructure, thermal behavior and magnetic response were checked by X-ray diffraction (XRD), differential scanning calorimetry (DSC) and vibrating sample magnetometry (VSM). After 80 h of MA, the alloys were nanostructured (bcc-Fe(Co)-rich phase). As the Co content increases, the density of the dislocations decreases. Besides, a higher concentration of Co causes an increase in the activation energy of the crystallization process. The calculated energies, 267 and 332 kJ/mol, are associated to the crystalline growth of the bcc-Fe-rich phase. The Co content of the samples has no effect on the value of the saturation magnetization, whereas the coercivity is lower in the alloy containing less Co. Samples were compacted and heat-treated. Optimal annealing reduces the coercivity by a factor of two. Results were compared with the data of Fe-Nb-B and Fe-Ni-Nb-B alloys.The study presents technological and structural aspects of production and heat treatment of welded joints of high-strength, abrasion-resistant Hardox 600 steel. As a result of the conducted research, it was found that the use of welding processes for joining this steel leads to the formation of a wide heat-affected zone, characterized by various structures favoring the reduction of abrasion resistance and deterioration of plastic properties, while increasing the susceptibility to brittle fracture. On the basis of the structural and strength characteristics, an effective welding technology for Hardox 600 steel was proposed, as well as the conditions and parameters of post-welding heat treatment, leading to obtaining structures close to a base material in the entire area of the welded joint. Despite the limited high-carbon equivalent CEV of the metallurgical weldability of the tested steel, the tests carried out in laboratory conditions allowed researchers to obtain welded joints characterized by very high strength indexes, corresponding to the base material, while maintaining satisfactory plastic and impact properties.The suitability of a new type of polyurethane-based composite carbon foam for several possible usages is evaluated and reported. A comparison of the properties of the as-prepared carbon foams was performed with widely available commercial biomass-derived activated carbon. Carbon foams were synthesized from polyurethane foams with different graphite contents through one-step activation using CO2. In this work, a carbon catalyst was synthesized with a moderately active surface (SBET = 554 m2/g), a thermal conductivity of 0.09 W/mK, and a minimum metal ion content of 0.2 wt%, which can be recommended for phosgene production. The composite carbon foams exhibited better thermal stability, as there is a very little weight loss at temperatures below 500 °C, and weight loss is slower at temperatures above 500 °C (phosgene synthesis 550-700 °C). Owing to the good surface and thermal properties and the negligible metallic impurities, composite carbon foam produced from polyurethane foams are the best alternative to the conventional coconut-based activated carbon catalyst used in phosgene gas production.A set of manganese oxide catalysts was synthesized via two preparation techniques solution combustion synthesis (Mn3O4/Mn2O3-SCS and Mn2O3-SCS) and sol-gel synthesis (Mn2O3-SG550 and Mn2O3-SG650). The physicochemical properties of the catalysts were studied by means of N2-physisorption at -196 °C, X-ray powder diffraction, H2 temperature-programmed reduction (H2-TPR), soot-TPR, X-ray photoelectron spectroscopy (XPS) and field-emission scanning electron microscopy (FESEM). The high catalytic performance of the catalysts was verified in the oxidation of Volatile Organic Compounds (VOC) probe molecules (ethene and propene) and carbon soot in a temperature-programmed oxidation setup. The best catalytic performances in soot abatement were observed for the Mn2O3-SG550 and the Mn3O4/Mn2O3-SCS catalysts. The catalytic activity in VOC total oxidation was effectively correlated to the enhanced low-temperature reducibility of the catalysts and the abundant surface Oα-species. Likewise, low-temperature oxidation of soot in tight contact occurred over the Mn2O3-SG550 catalyst and was attributed to high amounts of surface Oα-species and better surface reducibility. For the soot oxidation in loose contact, the improved catalytic performance of the Mn3O4/Mn2O3-SCS catalyst was attributed to the beneficial effects of both the morphological structure that-like a filter-enhanced the capture of soot particles and to a probable high amount of surface acid-sites, which is characteristic of Mn3O4 catalysts.In this work, we are interested in the nucleation of bâtonnets at the Isotropic/Smectic A phase transition of 10CB liquid crystal. Very often, these bâtonnets are decorated with a large number of focal conics. We present here an example of a bâtonnet obtained by optical crossed polarized microscopy in a frequently observed particular area of the sample. This bâtonnet presents bulges and one of them consists of a tessellation of ellipses. These ellipses are two by two tangent, one to each other, and their confocal hyperbolas merge at the apex of the bâtonnet. We propose a numerical simulation with Python software to reproduce this tiling of ellipses as well as the shape of the smectic layers taking the well-known shape of Dupin cyclides within this particular bâtonnet area.