Bankedennis2459

In the Kaplan-Meier analysis of the combined endpoint time to liver transplantation or death, we observed that patients with the PNPLA3-rs738409 GG variant met more frequently the primary endpoint (P = 0.005). In Cox regression analysis PNPLA3-rs738409 GG as well as liver cirrhosis were identified as strong predictors for time to liver transplantation or death (HR 4.5 [CI 1.48-13.72], P = 0.008 and HR 9.24 [CI 2.11-40.44], P = 0.003, respectively). Neither steatosis, diabetes mellitus nor obesity were associated with outcome. CONCLUSIONS PNPLA3-rs738409 variant GG is a predictor for time to liver transplantation or death and may help to identify autoimmune hepatitis patients at risk for disease progression. © 2020 The Authors. Alimentary Pharmacology & Therapeutics published by John Wiley & Sons Ltd.BACKGROUND The third-generation laser balloon (LB3) became available recently. We aimed to describe the impact on procedural efficacy and safety of LB3 compared to the second generation LB2 for pulmonary vein (PV) isolation (PVI) in patients with atrial fibrillation (AF). METHODS Consecutive patients treated with the LB3 for symptomatic AF were enrolled. As a control group the previous consecutive patients treated with the LB2 were analyzed. Acute procedural data and complications between two groups were retrospectively analyzed. RESULTS A total of 84 patients with LB3 (317 PVs, 75.9% Paroxysmal AF) and the previous 100 patients (387 PVs, 63.0 % Paroxysmal AF) with LB2 who underwent PVI were enrolled in this study. LB3 enabled significantly shorter procedural time (LB3 60.6 ± 22.0 min vs LB2 100.9 ± 21.5 min, P less then 0.0001) and fluoroscopic time (LB3 7.2 ± 3.5 min vs LB2 8.6 ± 3.2 min, P = 0.0046). Complete PV isolation after initial circular ablation was achieved in 75.0% with LB3 and 77.7% with LB2 (P = 0.725). Optimal PV occlusion grade was the only independent predictor of successful PVI after initial circular LB3 ablation (OR 0.190; 95% CI 0.062 - 0.572; P = 0.0035). Balloon pinholes were recorded more often in LB3 group (LB3 19.1% vs LB2 7%, P = 0.0236) CONCLUSION LB3 confirmed a high rate of visually guided PVI, foreshortening procedure duration and fluoroscopy use. However, the use of LB3 was associated with a considerable rate of pinhole balloon ruptures. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.Problematic sea lice infestations on farmed Atlantic salmon (Salmo salar) have motivated extensive research and development into new methods to prevent, monitor and control sea lice. Most of these technologies require detailed information on the behaviour, spatial distribution and demography of lice on host fish. This study investigated how salmon lice (Lepeophtheirus salmonis) infestation density varies across the host's surface under sea cage farming conditions. Lice abundance, demography and attachment location were tracked over time, with repeated sampling of 300 individually tagged salmon across three replicate experimental sea cages. The data reveal clear differences in attachment locations according to sex and stage, but with an overall preference for the dorsal surface among mobile stages-dorsal head for adult females and dorsal-posterior section for males and pre-adults. Total lice abundance was highly variable between repeated measures of individual fish, consistent with frequent host-switching or mortality. Total lice numbers also declined between sampling dates, likely due to handling, with lost mobile lice being almost exclusively adult males. As the distribution of sea lice on hosts is likely determined by numerous factors, future image-based automated detection systems should be validated in settings that reflect the complex host-parasite interactions that occur in open farming systems. click here © 2020 The Authors. Journal of Fish Diseases published by John Wiley & Sons Ltd.Intracellular protein degradation is essential for the survival of all organisms, but its role in interspecies interaction is unknown. Here, we show that the ClpXP protease of Pseudomonas aeruginosa suppresses its antimicrobial activity against Staphylococcus aureus, a common pathogen co-isolated with P. aeruginosa from polymicrobial human infections. Using proteomic, biochemical, and molecular genetic approaches, we found that this effect is due to the inhibitory effects of ClpXP on the quorum sensing (QS) of P. aeruginosa, mainly by degrading proteins (e.g., PhnA, PhnB, PqsR, and RhlI) which are critical for the production of QS signal molecules PQS and C4-HSL. We provide evidence that co-culturing with S. aureus induces a decrease in the activity of ClpXP in P. aeruginosa, an effect which was also achieved by the treatment of P. aeruginosa with N-acetylglucosamine (GlcNAc), a widespread chemical present on the surface of diverse cell types from bacteria to humans. These findings extend the range of biological events governed by proteolytic machinery to microbial community structure, thus also suggesting that a chemical-induced alteration of protein homeostasis is a mechanism for interspecies interactions. © 2020 John Wiley & Sons Ltd.Subcutaneous granuloma annulare is a rare variant of granuloma annulare. It mostly presents as painless subcutaneous nodule(s) with or without annular lesions on the lower legs and scalp of children. We herein report a case of a 5-year-old Japanese girl with subcutaneous granuloma annulare on the right heel. The lesions improved in 15 months without any therapy. We also review 85 reported cases of subcutaneous granuloma annulare between 1 and 85 years of age in the Japanese published work. The most susceptible age was 5 years and below; however, a small peak occurred from the 40s to 60s. Subcutaneous granuloma annulare was more frequent in females (male  female ratio, 611). Sixty-five cases (76.4%) had multiple lesions; the average number of lesions was 4.8 ± 5.8. The most affected site was the lower legs (28 cases), followed by the dorsum of the foot (21 cases) and scalp (17 cases). The heel was an unusual location; only two cases of sole subcutaneous granuloma annulare have been reported. Twenty-one cases revealed a classical dermal change along with a subcutaneous change.