Balslevgustafsson5973

To characterize long-term strabismus outcomes in children in the Infant Aphakia Treatment Study (IATS).

This study was a secondary data analysis of long-term ocular alignment characteristics of children aged 10.5 years who had previously been enrolled in a randomized clinical trial evaluating aphakic management after unilateral cataract surgery between 1 and 6 months of age.

In the IATS study, 96 of 109 children (88%) developed strabismus through age 10.5 years. Half of the 20 children who were orthophoric at distance through age 5 years maintained orthophoria at distance fixation at 10.5 years. Esotropia was the most common type of strabismus prior to age 5 years (56/109 [51%]), whereas exotropia (49/109 [45%]) was the most common type of strabismus at 10.5 years (esotropia, 21%; isolated hypertropia, 17%). Strabismus surgery had been performed on 52 children (48%), with 18 of these (35%) achieving microtropia <10

. Strabismus was equally prevalent in children randomized to contact lens care comparnt.

To assess the relationship between baseline factors and time to diabetic macular edema (DME) resolution.

Post hoc analysis of VISTA and VIVID.

Eyes with baseline central subfield thickness (CST) of ≥ 290 μm.

Eyes were treated with intravitreal aflibercept injection (IAI) 2 mg (n= 558; every 4 weeks or every 8 weeks after 5 monthly doses) or laser control (n= 274). The effect of baseline factors on the time to DME resolution (CST < 290 μm) was assessed in univariable and multivariable models and further evaluated by the Kaplan-Meier method.

Time to and cumulative incidence of DME resolution.

Eyes treated with IAI had a 2.5-fold higher DME resolution rate, with median time of 33.0 weeks (95% confidence interval [CI], 28.1-40.0), compared with DME resolution not being achieved in 50% of eyes treated with laser control. Multivariable analysis demonstrated that a lower DME resolution rate was associated with a thicker baseline CST (hazard ratio [HR] [95% CI] per 100-μm CST increase, 0.79 [0.72-0.86]e IAI group were associated with a longer time to and a lower rate of DME resolution.

The median time to DME resolution was 33 weeks among IAI-treated eyes. A thicker baseline CST and better baseline BCVA in the IAI group were associated with a longer time to and a lower rate of DME resolution.

To investigate late vitreoretinal complications and visual outcomes in patients with regressed retinopathy of prematurity (ROP) with or without prior treatment.

International, multicenter, noncomparative retrospective case series.

We analyzed 264 eyes of 238 patients from 13 centers worldwide who developed vitreoretinal complications (retinal detachment [RD], vitreous hemorrhage [VH], or retinal break) ≥ 2 years after resolution of acute ROP.

Each participant was assigned to 1 of 3 groups (the RD, VH, and retinal break groups) according to their primary diagnosis. The average age at presentation, visual acuities, refractive error, axial length, gestational age, birth weight, acute ROP classification, prior treatments for acute ROP, postoperative visual acuity (VA), and concomitant eye conditions in the 3 groups were documented and compared.

Clinical features and visual outcomes of late vitreoretinal complications in patients with regressed ROP.

A total of 264 eyes of 238 patients were included. Thre detected, timely surgical intervention is necessary to ensure favorable visual outcomes in these patients.

Infants with acute ROP remain at a high risk of vision-threatening complications throughout childhood and adulthood. Continual follow-up of patients with ROP is important. When severe complications, such as RD or VH, are detected, timely surgical intervention is necessary to ensure favorable visual outcomes in these patients.The osmoregulation system of freshwater fish is sensitive to salinity increase in water. There is no satisfactory data to our knowledge on the accumulation of metal-oxide nanoparticles (NPs) in tissues of O. niloticus and their effects on ATPases (Na,K-ATPase, Mg-ATPase, Ca-ATPase) in differing salinities. Thus, this study investigated the effects of salinity (0 and 10 ppt) and Al2O3 and TiO2 NPs (1 and 10 mg NPs/L) on the response of ATPases in acute (2 days) and chronic (20 days) durations. see more Data showed that nanoparticles accumulated in the tissues of fish, gill tissues having the highest levels of Al and Ti in both acute and chronic durations. Interestingly, the higher salinity significantly increased (P less then 0.05) NP accumulations in the tissues in acute exposures, whereas it significantly decreased (P less then 0.05) in chronic exposures. Salinity increase caused significant decreases (P less then 0.05) in ATPase activities (up to 54 %) in control fish from both exposure protocols. Likewise, NP alone exposures (up to 80 %) and salt+NP (up to 83 %) exposures generally caused significant (P less then 0.05) decreases in ATPase activities compared to their controls. Similarly, salt+NP exposures also decreased ATPase activities compared to NP exposures alone. The present data demonstrated that salinity and/or NP exposures decreased ATPase activities in the gill of freshwater fish, emphasizing the possible hazardous consequences of salt inputs and NP discharges into freshwater systems.Sarcopenia is one of the most important health issues in today's ageing society. As an evaluation method, computed tomography (CT) is an effective means of assessing not only the quantity but also the quality of skeletal muscle. We aimed to examine the relationship between sarcopenia severity and muscle/fat area, and osteoporosis. 321 patients (116 men and 205 women, mean age 77.2 ± 7.1 years, age range 53-96 years) who visited the Integrated Healthy Aging Clinic from 2016 to 2017 were included in this study. Based on the Asia Working Group for Sarcopenia2019 criteria, patients were divided into four groups normal group, low-functional group (with normal skeletal muscle mass, but reduced muscle strength or physical function), sarcopenia group, and severe sarcopenia group. We measured the skeletal muscle (SM), intermuscular adipose tissue (IMAT), and subcutaneous adipose tissue (SAT) areas and the CT attenuation values (CTV) using cross sections of the mid-thigh CT. We also measured bone mineral density. Then, we compared each result among the four groups. We found a significant decrease in SM area in both men and women with sarcopenia (p less then 0.001 for both sexes). In women, a decrease in SAT area was observed in the sarcopenia group (p less then 0.001), and an increase in IMAT was observed in the low functional group (p less then 0.001). The CTV decreased in men with sarcopenia and severe sarcopenia; similarly, women in the low functional and severe sarcopenia groups had decreased CTV (p less then 0.001 for both sexes). An association between sarcopenia and osteoporosis in men was detected (p = 0.004). By using not only muscle mass but also fat mass and CTV, we were able to better examine the pathogenesis of sarcopenia and differences between men and women in Japanese middle-aged and older adults.

To estimate the genetic contribution to doctor-diagnosed hand osteoarthritis (OA).

Using data from the Swedish Twin Registry and National Patient Register, we conducted a 20-year population-based longitudinal cohort study including 59,970 twins aged 35 years or older. We studied inpatient and outpatient doctor-diagnosed hand OA using ICD-10 codes from 1997 until 2016, including both the distal/proximal interphalangeal (DIP/PIP) joints and/or the first carpometacarpal (CMC-1) joints. We calculated intra-pair correlation, estimated the heritability (i.e., the percentage variation in hand OA that can be explained by genetic factors) as well as a genetic risk.

Among 59,970 included persons, 936 had a hand OA diagnosis registered during the study period. The heritabilities of hand OA (any joint), CMC-1 OA and DIP/PIP OA were ∼87%, 86% and 48%, respectively, yet the two latter should be interpreted with care due to low numbers. Hand OA in any joint in both twins in a pair occurred more frequently in identical twins (54/554=9.7%, intra-pair correlation=0.54, 95% CI=0.44-0.63) than in fraternal twins (18/1,246=1.4%, intra-pair correlation=0.10, 95% CI=-0.01-0.22). Identical twins who were diagnosed with hand OA in any joint had a far higher risk than fraternal twins with hand OA to also have their co-twin diagnosed with hand OA in any joint (Hazard Ratio=6.98, 95% CI=3.08-15.45).

The genetic contribution to hand OA is high and likely varying between 48% and 87%. Potential differential heritability by hand OA phenotypes should be further explored.

The genetic contribution to hand OA is high and likely varying between 48% and 87%. Potential differential heritability by hand OA phenotypes should be further explored.

Nerve growth factor (NGF) and sensory nerves are key factors in established osteoarthritis (OA) knee pain. We investigated the time course of NGF expression and sensory nerve growth across early and late stages of OA progression in rat knees.

Knee OA was induced by medial meniscectomy in rats. OA histopathology, NGF expression, and calcitonin gene-related peptide immunoreactive (CGRP-IR) nerves were quantified pre-surgery and post-surgery at weeks 1, 2, 4 and 6. Pain-related behavior was evaluated using dynamic weight distribution and mechanical sensitivity of the hind paw.

NGF expression in chondrocytes increased from week 1 and remained elevated until the advanced stage. In synovium, NGF expression increased only in early stages, whereas in osteochondral channels and bone marrow, NGF expression increased in the later stages of OA progression. CGRP-IR nerve density in suprapatellar pouch peaked at week 4 and decreased at week 6, whereas in osteochondral channels and bone marrow, CGRP-IR innervation inchat NGF is a key driver of articular nerve growth associated with OA pain.

Current study aimed at comparing the human dental pulp-derived stem cell (hDPSC) secretome (Control secretome) and transforming growth factor beta1 (TGF-β1)-transfected hDPSC secretome (TGF-β1 Secretome), which have the potential to be therapeutic in terms of regenerative dentistry, in terms of osteogenesis, adipogenesis and gingival wound healing with proteomic analyses.

pCMV-TGF-β1 plasmid was transfected into hDPSCs by electroporation. hDPSC and TGF-β1 transfected hDPSC secretomes were collected for LC-MS/MS. Protein contents in control secretome and TGF-β1 secretome were analyzed by tandem mass spectrometry-based shotgun proteomic method. Bioinformatic evaluations for canonical pathways, upstream regulators and networks were completed via Ingenuity Pathway Analysis (IPA, QIAGEN) software. Surface marker expressions between groups, treated secretome were measured by flow cytometry. To support the proteomic data morphologically, we performed osteogenic-adipogenic differentiation in hDPSCs treated with cund healing of oral mucosa and gingival tissue. TGF-β1 secretome may be a potential cell-free therapeutic in orthopedics and regenerative dentistry.

Based on these results, TGF-β1 secretome may have a therapeutic effect in repairing osteoporosis-related bone injuries, wound healing of oral mucosa and gingival tissue. TGF-β1 secretome may be a potential cell-free therapeutic in orthopedics and regenerative dentistry.