Ayersayala8438

 foliolosum. Ethnomedicinal studies revealed much wider scope of T. foliolosum in developing various drugs to solve multiple challenges in the health sector. Therapeutic effects were attributed to the bioactivities of the secondary metabolites present in T. foliolosum. CONCLUSIONS T. foliolosum is rich in berberine and other benzylisoquinoline alkaloids. T. foliolosum can be used as an excellent and effective herbal remedy for various human ailments since there are no reports on the toxicity of this herb. V.ETHNOPHARMACOLOGICAL RELEVANCE Anoectochilus Roxburghii (AR) has been used as a health food and a herb for the curative effect of longevity in China for hundreds years. AIM OF THE STUDY Pharmacological studies demonstrated that the plant ethanol extract possessed anti-oxidative and anti-aging effects on natural aging mice. However, it has not been reported whether the AR flavonoids extract (ARF) has a protective effect on oxidative stress in LO2 cells induced by H2O2 and D-gal induced aging mouse model. MATERIALS AND METHODS In this study, H2O2-induced oxidative stress LO2 cell model was performed to investigate the protective effect of ARF against H2O2 - induced oxidative damage in the LO2 cells by the method of MTT, the content of MDA and SOD, GSH-PX activities were determined. In D-gal induced aging mouse model, we observed behavioral assessment using the Morris water maze (MWM) and pathological morphology of the skin and liver, detected the biomarkers (SOD, GSH-PX, MDA, MAO, AChE) in serum, liver, brain and antioxidant-related gene expression in liver. RESULTS The data showed that ARF had certain protective effect on LO2 cells induced by H2O2 and effectively increased activities of SOD, GSH-PX, and decreased MDA content in cell supernatant. In vivo, ARF improved memory and the pathological morphology of skin and liver tissue, decreased MDA content, increased SOD, GSH-PX and MAO activity in varying degrees, and upregulated GPx-1 and GPx-4 expression in the aging mice induced by D-gal. CONCLUSIONS ARF protects LO2 cells against H2O2 induced oxidative stress and exerts the potent anti-aging effects in D-gal aging mouse model, and can provide reference for the development of AR as food additives and nature antioxidants in the future. V.ETHNOPHARMACOLOGICAL RELEVANCE Xiaoyaosan (XYS), a represent and classic TCM prescription, consists of Radix Bupleuri, Radix Angelicae Sinensis, Radix Paeoniae Alba, Rhizoma Atractylodis Macrocephalae, Poria, Herba Menthae, Rhizoma Zingiberis Recens and Radix Glycyrrhizae. XYS can sooth the liver and strengthen the spleen through improving the circulation of qi and nourishing blood according to the TCM theory, therefore exhibiting anti-depression effects. AIM OF THE STUDY This study was conducted to investigate the compatibility rule of antidepressant effect of XYS by using both the "Efficacy Compositions" research strategy and fecal metabolomics approach. MATERIALS AND METHODS XYS was divided into two efficacy groups, i.e. the Shugan (SG) and the Jianpi (JP) groups, according to the efficacies of both XYS and the eight herbs recorded in the TCM theory and the research strategy of "Efficacy Compositions". A CUMS-induced depression model was constructed, where rats were randomly divided into 5 groups negative tegrated antidepressant effect of XYS. This study suggests that a combination of "Efficacy Compositions" strategy and metabolomics approach has great potentials in comprehensively and deeply understanding the scientific connotation of the compatibility rule of TCM prescriptions. ETHNOPHARMACOLOGICAL RELEVANCE Dalbergia pinnata (Lour.) Prain (D. pinnata) is a plant widely distributed in tropical and subtropical regions of Asia, Africa, and the Americas. In humans, it is used in the prevention and treatment of diseases such as respiratory system, digestive system, cardiovascular and cerebrovascular diseases. AIM OF THE STUDY This study was aim to evaluate chemical composition, antioxidant activities, antimicrobial, and anti-melanogenesis properties of Essential oils (EO) from D. pinnata. MATERIALS AND METHODS In this paper, the EO of D. pinnata were extracted using the supercritical CO2 extraction method and purified by molecular distillation. The volatile compounds of EO were characterized using Gas Chromatography-Mass Spectrometer (GC-MS). The antioxidant activities were evaluated by the methods of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assays. And two Gram-positive bacteria, three Gr were slightly affected by the EO. Additionally, EO from D. pinnata could reduce tyrosinase activity and melanin synthesis of zebrafish embryos in dose-dependent manner. And EO exhibited the more obvious anti-melanogenic effect compared with the positive control arbutin at the same dose (30 mg/L). CONCLUSIONS Our results validated the main activities attributed to D. selleck inhibitor pinnata for its antimicrobial and antioxidant. In addition, the potent inhibitory impacts of EO on the pigmentation provides a theoretical basis for the in-depth study of the EO from D. pinnata and their application in pharmaceutical and cosmetic industries. Search for natural substances in association with conventional chemotherapeutic drugs with a chemiosensitizing action easily accessible to the tumor mass has encouraged our studies on voacamine (VOA) and its monomeric units, voacangine and vobasine. Our previous results showed that VOA sensitized multidrug resistant (MDR) osteosarcoma cells (U-2 OS/DX) to doxorubicin (DOX) cytotoxicity. VOA, extracted by Peschiera fuchsiaefolia plant, is a bisindole alkaloid consisting of an Iboga skeleton (voacangine) directly linked to a 2-acyl indole unit (vobasine). High-performance thin-layer chromatography densitometry demonstrated the purity of VOA, voacangine and vobasine samples. Flow cytometry analysis showed that VOA, voacangine and vobasine enhanced DOX accumulation of U-2 OS/DX cells, in equally way, whereas VOA reduced more efficiently DOX efflux. Optical microscopy and clonogenic assay confirmed that VOA was more effective than voacangine and vobasine in enhancing DOX cytotoxic effect. These results showed that monomers linked together are necessary to modulate resistant phenotype of osteosarcoma cells.