Atkinsonjensen6562

Cardiovascular disease is a global problem that seriously endangers human health and life. At present, hypertension is considered to be a polygenic disease caused by the interaction of environmental factors and genetic factors. Various environmental factors have been proved to promote the occurrence and development of cardiovascular disease. MicroRNA (miR), as an essential gene regulatory factor in vivo, has been confirmed to participate in the regulation of many cell pathways, and its abnormal expression is closely related to a variety of human diseases. MiR-485-5p is located at 7q22.1, which has been proved to play an essential role in the tumor and cardiovascular system. Therefore, this paper discussed the mechanism of miR-485-5p on the morphology and function of the cardiovascular system. It is believed that miR-485-5p will impact the morphology and function of the cardiovascular system. Therefore, in the current study, 1655 unrelated patients with cardiovascular system diseases were simulated and analyzed based on the above background. A double luciferase reporter gene detection system verified the combination of miRNAs target recognition. The results showed that miR-485-5p significantly inhibited the luciferase activity of pGL-miR-wt but had no effect on pGL6-miR-mut. The lack of miR-485-5p can promote the activation of cardiac fibroblasts. The findings of this study can provide a new understanding and direction for the study of cardiovascular system morphology and function.Percutaneous transluminal coronary angioplasty (PTCA) has been accepted as the elective treatment in many patients with coronary atherosclerotic obstruction. A slight increase in cardiac markers after the percutaneous coronary intervention (PCI) has been commonly reported. Some researchers have suggested that it predicts mortality and long-term complications. This study aimed to evaluate the occurrence of increased postoperative cardiac enzymes and determine the relationship between such an increase and clinical angiographic and technical variables. For this purpose, the descriptive study was performed in Hospital's cardiac ward from 2020-to 2021. One hundred twenty-two patients with stable coronary artery disease were studied for elective PTCA implanted with successful and uncomplicated stenting. Blood samples were taken from all patients to measure cardiac markers 20 hours after surgery. The normal range was CTnI ≤ 2ng/ml and CKMB ≤ 24 IU/L. Plasma levels of myocardial infarction and their relationship withre is an increase in enzymes after successful and uncomplicated PCI selection. Increased CTnI occurs more frequently than CKMB. There is no relationship between enzyme enhancement and other clinical, angiographic, and technical variables.N6-methyladenosine (m6A) is the most common internal modification in mammalian mRNAs while RNA-binding motif protein 15 (RBM15) is an important methyltransferase in m6A modification. Increasing evidences have shown that RBM15 has a close correlation with lung cancer. However, specific functions of RBM15 in lung adenocarcinoma (LUAD) are limited. RBM15 expression was analyzed in human LUAD tissues and matched healthy lung tissue. RBM15 was knocked down via siRNA in A549 and H1734 cells. The relationships between RBM15 with cellular functions characteristics and mRNA m6A levels were explored. We performed functional characterization in A549 and H1734 cells lines to elucidate the molecular role of RBM15. Results found that RBM15 was up-regulated in the LUAD tissue and cells, which was linked to poor survival of LUAD patients. RBM15 can be knocked down via siRNA in A549, which leads to the exploration of the associations between RBM15 with cell characteristics. In vivo, RBM15 knockdown could decrease the methylation level, reduce proliferation, accelerate apoptosis and inhibit tumor growth. Our research shows that RBM15 facilitates LUAC cell progression by m6A demethylation. However, it is necessary to conduct further researches on potential downstream molecular mechanisms and m6A modification of RBM15 activity in LUAC.MicroRNAs (miRNAs) have been documented to function differently in numerous human cancers. TP-0184 Our study planned to investigate the role of microRNA-140 (miR-140) and to identify its possible target in osteosarcoma (OS) to predict their mechanism in OS. The miR-140 was down-regulated in OS, and its high expression decreased MG63 cell proliferation. At the molecular level, Wnt1 was a target of miR-140, and its expression could be suppressed by miR-140. Besides, miR-140 overexpression decreased drug resistance in OS cells treated by doxorubicin. Collectively, overexpression of miR-140 may inhibit human OS cell proliferation and may enhance drug sensitivity by direct regulation of Wnt/β-catenin signaling.This study aimed to investigate the effects of miR-145-5p on cardiomyocyte proliferation and apoptosis, GIGYF1 expression, inflammation, and oxidative stress in rats with myocardial ischemia-reperfusion injury (IRI). For this purpose, SPF male SD rats were used for IRI modeling. Experimental animals were subjected to specimen sampling and myocardial HE staining. The relative expression of miR-145-5p was detected by qRT-PCR; the protein expressions of GIGYF1, p-AKT, p53, Bax, p38MAPK, and ERK1/2 were detected by Western blot. Mouse embryonic cardiomyocytes H9C2 were used for H/R modeling, which was then subjected to cell transfection according to different grouping protocols. The target of miR-145-5p was confirmed to be GIGYF1 by dual-luciferase reporter assay. Further experiments were performed to detect the survival rate of transfected cells, the apoptosis of transfected cells, SOD activity determination, as well as IL-1β and IL-6 concentrations. The results showed that the expression level of miR-145-5p wasions of P38MAPK, p53, and Bax (all P less then 0.05), while the above trends were reversed following the simultaneous upregulation of miR-145-5p and GIGYF1 (all P less then 0.05). In general, our study confirmed a decreased expression of miR-145-5p and increased expression of GIGYF1 in the IRI or H/R model in vivo and in vitro. Overexpression of miR-145-5p can downregulate the expression of GIGYF1, further promote cell proliferation, inhibit cell apoptosis, alleviate inflammation and oxidative stress, and hence exert a protective role in myocardial infarction IRI.This research was conducted in order to investigate the role of miR-19b-3p in the development of osteoporosis (OP) in rats and the associated mechanisms. This study measured the expression levels of miR-19b-3p and IGF-1 in clinical OP patients and ovariectomy-induced OP rats by qRT-PCR. The osteoprotegerin levels in OP patients were measured by enzyme-linked immunosorbent assay (ELISA). The binding site of miR-19b-3p to IGF-1 was predicted by three prediction sites Target Scan, miRDB and starbase. Experiments were conducted in vitro and in vivo using bone marrow mesenchymal stem cells (BMSCs) and OP rats, respectively, to verify the regulatory relationship between miR-19b-3p and IGF-1 and explore the role of miR-19b-3p in the development of OP. Results showed that the expression of miR-19b-3p was elevated in OP patients and rats, while IGF-1 expression was decreased (***p less then 0.001). The ELISA assay found that osteoprotegerin levels were inversely correlated with miR-19b-3p and positively correlated with IGF-1. The predictive analysis identified binding sites for miR-19b-3p to IGF-1. The potential regulatory relationship between miR-19b-3p and IGF-1 was validated by in vitro and in vivo experiments. Moreover, the important role of miR-19b-3p in the regulation of OP was further demonstrated. It was concluded the inhibition of miR-19b-3p has a suppressive effect on the development of OP and the function of miR-19b-3p in OP is likely to be achieved by regulating the expression of the IGF-1 gene.This study aimed to analyze the effects of 5A nursing (including Ask, Assess, Advise, Assist, and Access) combined with psychological nursing on the immune function, cancer-related fatigue, and complications of patients undergoing radical resection of colorectal cancer. For this purpose, 64 patients undergoing radical resection of colorectal cancer were treated with nursing intervention during the perioperative period from March 2019 to March 2021. They were randomly divided into a control group and a study group according to admission order. Each group had 32 cases. The control group performed routine nursing, and 5A nursing combined with psychological nursing was performed on the research group. Then the immune function, cancer-related fatigue, complications, self-efficacy, and nursing satisfaction were compared between the two groups. Results showed that the levels of CD3+ and CD4+ in the two groups were lower than before nursing, and the levels of CD8+, IgA, IgM, and IgG were higher than before nursing (Pth psychological nursing can effectively relieve the perioperative psychological pressure of the patients, improve the immune function of the body, relieve cancer-related fatigue, prevent and control the incidence of complications, and improve the quality of clinical life of the patients for patients undergoing radical resection of colorectal cancer.This study aims to screen the differential expression of total RNA transcripts in formalin-fixed paraffin-embedded tissues (FFPETs) in breast cancer (BRCA) and normal adjacent tissues (NATs) and identify miR-191 as a new biomarker for early diagnosing BRCA. Differentially expressed genes (DEGs) by MACE-Seq and differentially expressed ncRNAs (DEncRNAs) by the TrueQuant technique were examined in this study. The miR-191 expression level was measured by Real Time-qPCR. An average of 4,739 coding genes from 25,713 significantly down-regulated genes was identified, whereas 3,954 coding genes were significantly up-regulated in the BRCA against NAT. An average of 1450 ncRNAs, including up-regulated= 679 and down-regulated= 780, were differentially expressed in 7 paired samples of BRCA and NAT. Among the ncRNAs, 227 microRNAs, including unchanged= 152, down=53, and up=22, were differentially expressed. MiR-191 was one of the 22 significant up-regulation, with p=0.0001. RT-qPCR results confirmed that miR-191, p=0.003, was significantly over-expressed in 120 paired samples of BRCA and NAT. Furthermore, NextSeq 500 revealed that a single nucleotide polymorphism (C>T) newly occurred in the mature sequence of miR-191-5p seed region in BRCA samples. However, the putative target genes regulated by the miR-191-5p were recognized by the above ten computational programs for the prediction. MACE-Seq outcomes showed that the genes of CDK6(P=0.0001), DAPK1(P=0.02), MTC7(P=0.04), SETD1B(P=0.005), CALN1(P=0.01), and TMOD2(P=0.001) were significantly over-expressed in the BRCA against the NATs. The expression level of the targets was adversely related to the miR-191-5p.The current study aimed to explore the correlation between Mir-34A-3p, Mir-31, PLEK2 and the occurrence, development and prognosis of colorectal cancer. For this paper, 120 patients with colorectal cancer were selected as the study group, and their adjacent normal tissues were selected as the control group. The quantitative real-time PCR (QRT-PCR) method was used to detect miR-34a-3p and miR-31 in tissues, and the immunohistochemistry EnVision two-step method was used to detect PLEK2 positive expression. The expressions of miR -34a-3p, miR -31, and PLEK2 in colon cancer tissues and normal cancer tissues were compared, and the correlation between miR -34a-3p, miR -31, and PLEK2 and clinic-pathological characteristics of colorectal cancer patients were analyzed. The results showed that expression of miR -34a-3p, miR -31 and positive expression rate of PLEK2 in colorectal cancer tissues were higher than those in normal adjacent tissues (P less then 0.05). The expression of miR -34a-3p was related to tumor size, degree of tissue differentiation, lymph node metastasis and TNM stage (P less then 0.