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85, sensitivity of 83.3% and specificity of 83.3% (p<0.001). Other hematologic ratios, such as the derived NLR (dNLR) and platelet-to-lymphocyte ratio (PLR) were also significant predictors for poor response, although to a lesser extent when compared to NLR.
NLR is a simple and cost-effective predictor for neoadjuvant treatment response in LARC. As more data is generated, clear cut-off values could provide valuable insight regarding the management of LARC.
NLR is a simple and cost-effective predictor for neoadjuvant treatment response in LARC. As more data is generated, clear cut-off values could provide valuable insight regarding the management of LARC.
To explore the associations of computed tomography (CT) features with tumor-node-metastasis (TNM) stage and pathology of patients with rectal cancer and their significance in the evaluation of efficacy and prognosis.
A total of 83 rectal cancer patients who were operated in our hospital were collected. CT examination was performed before operation, and pathological examination was conducted after operation. The influence of CT stage on the prognosis of patients with rectal cancer was assessed.
Postoperative pathological examination showed that there were 15 cases in T1-T2, 41 cases in T3 and 27 cases in T4. The results of CT examination showed that there were 15 cases in T1-T2, 40 cases in T3 and 28 cases in T4, and the sensitivity was 93.18%. It can be seen that the results in the two examinations were similar. The postoperative pathological examination revealed that lymph node metastasis occurred in 57 cases, and the main metastatic sites were the left and right pelvic cavity, near the intestine and ide metastasis. CT scan also has great value for distant metastasis, and contributes to the development of clinical therapeutic regimens for patients with rectal cancer in different stages. CT stage has an influence on the prognosis and patient survival.
Expression of programmed death ligand-1 (PD-L1) is related to the prognosis of many solid tumors, but the prognostic value of PD-L1 expression in colorectal cancer (CRC) remains unclear. The aim of this study was to clarify the role of PD-L1 expression in predicting prognosis, and then provide further insight into the relation between PD-L1 and toll like receptor-4 (TLR-4) in CRC progression.
The expression of PD-L1 and TLR-4 in patients with resected CRC was analyzed using immunohistochemistry (IHC). The biological relation of PD-L1 and TLR-4 in CRC was explored using gene set enrichment analysis (GSEA).
Positive PD-L1 and TLR-4 expression in tumor cells were observed in 12.7% and 41.2% respectively. High PD-L1 and TLR-4 expression levels were significantly correlated with poor disease-free survival. PD-L1 expression was closely associated with TLR-4 expression. Multivariate analyses further confirmed that increased expression levels of PD-L1 are unfavorable prognostic factors for operable CRC.
High PD-L1 expression can be used as a prognostic indicator for patients with operable CRC. PD-L1 expression is associated with TLR-4 expression, thereby providing a theoretical basis for the combined use of PD-1/PD-L1 inhibitors and TLR agonists.
High PD-L1 expression can be used as a prognostic indicator for patients with operable CRC. PD-L1 expression is associated with TLR-4 expression, thereby providing a theoretical basis for the combined use of PD-1/PD-L1 inhibitors and TLR agonists.
To explore the effectiveness of fluorouracil (FU) entrapped in dendritic cell (DC)-secreted exosomes (DC-Exos) for enhancing its anti-colon cancer effect.
DC-Exos were extracted through ultrahigh-speed centrifugation, and the FU-DC-Exos system was constructed using electroporation. Moreover, the influence of FU-DC-Exos on the viability of mouse colon cancer CT26 cells was determined in vitro using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and 4',6-diamidino-2-phenylindole (DAPI) staining, and pharmacodynamic evaluation was performed in vivo through TUNEL and hematoxylin-eosin (H&E) staining, with the CT26 tumor-bearing mouse model as the study object.
Compared with the control group, FU and DC-Exos groups exhibited a lowered proliferation rate of mouse CT26 colon cancer cells, whereas the apoptosis rate of cells in FU-DC-Exos group differed significantly from FU group. According to the wound healing assay, the migration rate of CT26 cells was reduced in FU and DC-Exos groups, and it was evidently different between FU-DC-Exos group and FU group. Moreover, in vivo experiments revealed that DC-Exos alone exhibited a trend of suppressing tumor growth, and that DC-Exos as carriers not only killed tumor cells alone, but also enhanced the anti-colon cancer effect of FU after entrapment.
Exos extracted via ultrahigh-speed centrifugation can be used for the preparation of the drug delivery system of FU-DC-Exos via electroporation, and drug-loaded Exos are able to effectively inhibit the proliferation of tumor cells and induce their apoptosis, exerting an anti-tumor effect.
Exos extracted via ultrahigh-speed centrifugation can be used for the preparation of the drug delivery system of FU-DC-Exos via electroporation, and drug-loaded Exos are able to effectively inhibit the proliferation of tumor cells and induce their apoptosis, exerting an anti-tumor effect.
The number of surgical operations for elderly patients with hepatocellular carcinoma increases as the population ages. The aim of this study was to evaluate surgical and survival outcomes in elderly patients who underwent laparoscopic or open hepatectomy for hepatocellular carcinoma.
We analyzed the data of 169 patients aged 70 or over who underwent hepatectomy for hepatocellular carcinom between January 2013 and December 2018. Sixty-four pairs were selected after propensity score matching for laparoscopic or open hepatectomy for hepatocellular carcinoma. Baseline data, surgery time, length of hospital stay, postoperative complications, pathological data, overall survival, and disease-free survival were investigated.
Operative time in the laparoscopic group was longer than in the open group. Blood loss and postoperative hospital stay were shorter in the laparoscopic group than in the open group. The rate of postoperative 30-day minor or major complications was similar between the two groups. There was no significant difference in pathological data between the two groups. There was no significant difference in overall survival and disease-free survival between the two groups.
This study suggests that laparoscopic hepatectomy for elderly patients with hepatocellular carcinoma may be safe and feasible, with better short-term outcomes and similar long-term outcomes.
This study suggests that laparoscopic hepatectomy for elderly patients with hepatocellular carcinoma may be safe and feasible, with better short-term outcomes and similar long-term outcomes.
To uncover the role of LINC01980 in aggravating the progression of hepatocellular carcinoma (HCC) via targeting caspase 9.
The expression levels of LINC01980 and caspase 9 in HCC tissues and paracancer tissues were determined by qRT-PCR. The prognostic potentials of LINC01980 and caspase 9 in HCC were assessed by Kaplan-Meier method. The regulatory effects of LINC01980 and caspase 9 on the viability, clonality and apoptosis of Huh7 and Hep3B cells were examined. Finally, the interaction between LINC01980 and caspase 9 was evaluated by performing dual-luciferase reporter gene assay and rescue experiments.
LINC01980 was upregulated in HCC tissues and cells. High level of LINC01980 indicated worse prognosis in HCC patients. Knockdown of LINC01980 could attenuate viability and clonality, but induced apoptosis in Huh7 and Hep3B cells. Caspase 9 was downregulated in HCC, and its high level predicted a better prognosis in HCC patients. Overexpression of caspase 9 achieved the same regulatory effects as LINC01980 knockdown on HCC cells. Caspase 9 was the downstream target for LINC01980, and its level was negatively regulated by LINC01980. In HCC, LINC01980 regulated HCC cell behaviors by downregulating caspase 9.
Upregulation of LINC01980 in HCC predicts a poor prognosis. LINC01980 aggravates the progression of HCC via downregulating caspase 9.
Upregulation of LINC01980 in HCC predicts a poor prognosis. LINC01980 aggravates the progression of HCC via downregulating caspase 9.
Liver cancer stem cells are associated with tumor progression, metastasis, and resistance to chemotherapy. Therefore, it is important to understand the proteins that support the tumor microenvironment. The suppression of ZEB2 results from inactivation of the Wnt/β catenin pathway. Like RBM38, it suppresses tumor outgrowth and helps increase the survival of cancer patients. However, no studies have examined the direct roles of ZEB2 and RBM38 in the tumor microenvironment.
We developed an early/advanced stage liver cancer mouse model using CD133+ cell injection that mimics liver cancer in all ways. Histology, Western blotting, and immunohistochemistry analyses were used to examine cancer progression.
Histologically, the early liver cancer showed microfoci structures; the advanced cancer showed distinct morphological changes with enlarged nucleoli and cell clumping. Immunohistochemical and Western blotting analyses of CD133 and ZEB2 proteins showed similar upregulated expression as the tumor progressed. However, RBM38 expression increased dramatically in early liver cancer but was downregulated in advanced liver cancer.
ZEB2 favors a tumor microenvironment that supports liver cancer stem cell proliferation, while RBM38 expression negatively affects the tumor microenvironment and restricts liver cancer stem cell proliferation.
ZEB2 favors a tumor microenvironment that supports liver cancer stem cell proliferation, while RBM38 expression negatively affects the tumor microenvironment and restricts liver cancer stem cell proliferation.
Liver cancer is one of the most common and highly malignant cancers of the digestive system. The main aim of the present research work was to investigate the anticancer action of rosmarinic acid - a naturally occurring plant secondary metabolite. Blasticidin S nmr We also investigated its effects on cell apoptosis, caspase activation, cell migration and cell invasion.
Cell viability of Hep-G2 liver cancer cells was evaluated by CCK-8 assay while apoptotic studies were carried out by fluorescence microscopy using Hoechst, acridine orange (AO)/ethidium bromide (EB) and Comet assays as well as using annexin-v/propidium iodide (PI) assay for apoptosis quantification. Western blot assay was used to study the effects of rosmarinic acid on apoptosis-related protein expressions including Bax, Bcl-2 and various caspases. In vitro wound healing assay was used to evaluate the effects on cell migration while transwell chambers assay with Matrigel was used to assess the effects of rosmarinic acid on cell invasion.
Rosmarinic acid cauc acid has a potential to inhibit in vitro cancer cell growth in Hep-G2 cells by triggering apoptosis, caspase activation and suppressing cell migration and invasion and as such this molecule could be developed as a possible anticancer agent provided further studies are carried out.
