Ashleydencker7646

Unicellular organisms like yeast can undergo controlled demise in a manner that is partly reminiscent of mammalian cell death. This is true at the levels of both mechanistic and functional conservation. Yeast offers the combination of unparalleled genetic amenability and a comparatively simple biology to understand both the regulation and evolution of cell death. In this minireview, we address the capacity of the nucleus as a regulatory hub during yeast regulated cell death (RCD), which is becoming an increasingly central question in yeast RCD research. In particular, we explore and critically discuss the available data on stressors and signals that specifically impinge on the nucleus. Moreover, we also analyze the current knowledge on nuclear factors as well as on transcriptional control and epigenetic events that orchestrate yeast RCD. Altogether we conclude that the functional significance of the nucleus for yeast RCD in undisputable, but that further exploration beyond correlative work is necessary to disentangle the role of nuclear events in the regulatory network. © 2020 Elsevier Inc. All rights reserved.Aberrant cell death signaling and oxidative stress are implicated in myriad of human pathological states such as neurodegenerative, cardiovascular, metabolic and liver diseases, as well as drug-induced toxicities. While regulated cell death and mild oxidative stress are essential during normal tissue homeostasis, deregulated signaling can trigger massive depletion in a particular cell type and/or damage tissues and impair organ function with deleterious consequences that manifest as disease states. If regeneration cannot restore tissue homeostasis, the severity of the disease correlates with the extent of cell loss. Cell death can be executed via multiple modalities such as apoptosis, necrosis, pyroptosis, necroptosis and ferroptosis, depending on cell autonomous mechanisms (e.g., reactive oxygen species production, calcium overload and altered proteostasis) and/or non-cell autonomous processes (e.g., environmental stress, irradiation, chemotherapeutic agents, inflammation and pathogens). Accordingly, the inhibition of aberrant cell death and oxidative stress together with activation of autophagy, a regulated self-degradation process, are progressively emerging as relevant cytoprotective strategies to sustain homeostasis. In this review, we summarize the current literature on the crosstalk between cellular redox state and cell fate signaling, specifically from the standpoint of autophagy and its role in the maintenance of tissue/organ homeostasis via regulating oxidative stress and the potential implications for the design of novel therapeutic strategies. © 2020 Elsevier Inc. All rights reserved.Autophagy, the process of macromolecular degradation through the lysosome, has been extensively studied for the past decade or two. Autophagy can regulate cell death, especially apoptosis, through selective degradation of both positive and negative apoptosis regulators. However, multiple other programmed cell death pathways exist. As knowledge of these other types of cell death expand, it has been suggested that they also interact with autophagy. In this review, we discuss the molecular mechanisms that comprise three non-apoptotic forms of cell death (necroptosis, pyroptosis and ferroptosis) focusing on how the autophagy machinery regulates these different cell death mechanisms through (i) its degradative functions, i.e., true autophagy, and (ii) other non-degradative functions of the autophagy machinery such as serving as a signaling scaffold or by participating in other autophagy-independent cellular processes. © 2020 Elsevier Inc. All rights reserved.The fate of a cell is determined by multiple signaling pathways in response to a range of stimuli. Probably the most prominent cell death mechanism is apoptosis which can be triggered by both internal stresses, as well as extracellular stimuli, and is executed by two well-characterized pathways, the intrinsic and the extrinsic apoptosis pathways. Although autophagy can also lead to cell death under certain circumstances, its major function is as a cell survival process. Given that the life of a cell is at stake, it is not surprising that there is significant molecular crosstalk between these pathways. The nature of these interconnections is diverse and ranges from protein-protein interactions and post-translational modifications through to the degradation of molecular components by different proteins and organelles. In this chapter we review these mechanisms in detail. © 2020 Elsevier Inc. All rights reserved.While programmed cell death was once thought to be exclusive to eukaryotic cells, there are now abundant examples of well regulated cell death mechanisms in bacteria. The mechanisms by which bacteria undergo programmed cell death are diverse, and range from the use of toxin-antitoxin systems, to prophage-driven cell lysis. Moreover, some bacteria have learned how to coopt programmed cell death systems in competing bacteria. Interestingly, many of the potential reasons as to why bacteria undergo programmed cell death may parallel those observed in eukaryotic cells, and may be altruistic in nature. These include protection against infection, recycling of nutrients, to ensure correct morphological development, and in response to stressors. In the following chapter, we discuss the molecular and signaling mechanisms by which bacteria undergo programmed cell death. We conclude by discussing the current open questions in this expanding field. © 2020 Elsevier Inc. All rights reserved.AIM To evaluate volumetric contrast-enhanced three-dimensional T1-weighted (CE 3D T1) turbo spin-echo (TSE) with variable flip angle (SPACE), CE 3D T1 volumetric interpolated breath-hold examination (VIBE) sequences with conventional CE-two-dimensional (2D)-T1 and 2D-T2-weighted imaging (WI) sequences in assessing perianal fistulas. METHODS AND MATERIALS Twenty-three patients with perianal fistula were included in this prospective study and underwent pelvic magnetic resonance imaging (MRI) at 3 T including fat-supressed CE 3D T1 SPACE, CE 3D T1 VIBE, axial and coronal 2D-T1WI together with 2D-T2WI sequences in the axial and coronal planes. Acquisition times were recorded. Performance of each sequence was evaluated in terms of image quality, presence of artefacts, lesion conspicuity, fistula type, presence of abscess, visibility of internal orifice, and number of internal orifices. Results were compared with post-surgical findings defined as the reference standard. RESULTS Both CE 3D T1 VIBE and CE 3D T1 SPACE were the best sequences to determine fistula type and in terms of image quality, artefacts, and determining locations of internal orifices. The number of internal orifices was detected correctly in 23 (100%) patients for CE 3D T1 SPACE and CE 3D T1 VIBE sequences, in 17 (73.9%) patients on CE-T1WI, and in seven (30.4%) patients on 2D-T2WI. Lesion conspicuity was higher for CE 3D T1 SPACE and CE 3D T1 VIBE sequences compared to the 2D sequences (p less then 0.05). The overall acquisition time for each 3D sequence was shorter compared to the 2D sequences combined. CONCLUSION CE 3D T1 SPACE and CE 3D T1 VIBE sequences may outperform conventional 2D sequences in the evaluation of perianal fistulas in terms of visibility and number of internal orifices with a shorter scanning time. INTRODUCTION Saw-related injuries can be found in all parts of the body. Especially hand saw-related injuries are frequently encountered in the literature. The aim of the study is to present our demographic data, treatment strategy and prevention of the saw-related injuries in the lower extremity. METHODS A retrospective analysis was conducted between 2010 and 2015 and only cases with saw-related lower extremity injuries were included in the study. These patients were analyzed according to demographic data, occupational status, procedures performed, hospitalization length of stay, return to work, and complications. RESULTS There were 48 male and 1 female patients; their mean age was 44.06 ± 13.6 (22-77) years. The average referral time after injury was 1.51 ± 0.16 (1-7) hour. The average hospitalization length of stay was 3.76 ± 3.9 (1-22) days. Fractures were also present in 13 (26.5%) patients. Postinjury complications occurred in 18 (36.7%) patients. 4 (8.2%) of the cases did not return to the job after the injury. The average return time to the work was 2.95 ± 1.5 (1-7) months. CONCLUSION Lower extremity saw-related injuries tend to be overwhelmingly male and most often seen distal to the extremity, especially on the medial side of the extremity. A significant number of patients did not return to the job after the injury. The aircraft environmental control system (AECS) plays a vital role in the normal operation of an airplane; thus, performance degradation of the AECS may result in failure and even catastrophic consequences. Because performance degradation is inevitable, performance degradation assessment is critical to provide information for appropriate maintenance actions. This paper proposed an integrated framework for performance degradation assessment of AECS by introducing the visual cognition theory into the specific method. First, an observer is used to generate a residual error, which contains degradation information of the AECS. Second, the generated residual error is transformed into a two-dimensional image based on a permutation method. Third, inspired by the multichannel characteristic (MCC), the transformed image is decomposed into several subbands based on the nonsubsampled contourlet transform (NCST) for multiscale and multidirectional feature extraction, thus capturing the precise characteristics of degradation. Then, inspired by the manifold sensing characteristic (MSC), the manifold space is established based on diffusion maps (DM) to reveal the intrinsic evolutionary law of health degradation. Finally, the geodesic distance is employed to represent the deviation between the current state and health baseline. As a health indicator, the confidence value (CV), which is derived from the geodesic distance, is capable of representing the degradation trend of the AECS. The whole process of degradation can be tracked in real time based on the proposed method. An AECS simulation model was established in MATLAB/Simulink, and two typical faults were introduced. The results based on simulation data demonstrate that the proposed assessment method can effectively reflect the degradation process of the AECS and the constructed health indicator is reasonable. selleck chemical INTRODUCTION Pancreatic cystic neoplasms (PCNs) are being incidentally detected at an increased rate due to increased CT and MRI usage. EUS is an emerging tool that can differentiate between benign and malignant features of pancreatic cysts. We hoped to identify the specific cross-sectional imaging findings and patient characteristics that warrant EUS referral. METHODS We conducted a retrospective case-control chart review, evaluating patients, who were diagnosed with pancreatic cysts and underwent EUS between January 1, 2010 and December 31, 2017. RESULTS EUS was found to change management when CT imaging found cyst size > 4 cm (OR = 4.07, p  3 cm (OR = 3.79, p  less then  0.001) and associated solid component to the cyst (OR = 5.95, p  less then  0.01). Additionally, patient characteristics, including age less than 50 years, male sex and 10-pack year smoking history were significantly associated with EUS change in management. DISCUSSION Our findings suggest that EUS referral should be coordinated based on the findings of specific HRFs, with support from high risk patient characteristics, rather than the accumulation of multiple HRFs, as suggested by existing guidelines.