Andrewsstephens2238

Karst aquifers are important drinking water resources, but highly vulnerable to contamination. Contaminants can be transported rapidly through a network of fractures and conduits, with only limited sorption or degradation, which usually leads to a fast and strong response at karst springs. During migration, contaminants can also enter less mobile zones, such as pools or water in intra-karstic sediments, or advance from conduits into the adjacent fractured rock matrix. As contaminant concentrations in the main flow path(s) decrease, contaminants may migrate back into the main flow path and reach the karst springs at low (but significant) concentrations over a long time span. This is the conventional interpretation for the oft-observed steep rising limb and the long-tailed falling limb of tracer breakthrough curves in karst systems. Here, field measurements are examined from an alpine karst system in Austria where a series of distinctive, long-tailed breakthrough curves (BTCs) of conservative tracers were observed over distances up to 7400 m. Recognizing that the conventional advection-dispersion equation (ADE) cannot usually quantify such behavior, two other modeling approaches are considered, namely the two-region non-equilibrium (2RNE) model, which explicitly includes mobile and immobile zones, and a continuous time random walk (CTRW) model, which is based on a physically-based, probabilistic approach that describes anomalous (or non-Fickian) transport behavior characteristic of heterogeneous systems such as karst. In most cases, the ADE and 2RNE models do not quantify the low concentrations at longer travel times. The CTRW, in contrast, accounts for the long-tailed breakthrough behavior found in this karst system. Grain filling is the final determinant of yield, and this process is susceptible to abiotic stresses. Salicylic acid (SA) regulates grain filling in rice plants. A comparative proteomic study was conducted to understand how SA mediates grain filling under soil drying (SD) condition. Zhefu802 and its near-isogenic line (NIL) were planted in pots in an artificial chamber. SA (100 mg L-1) was applied, followed by SD treatment (with a water potential of -30 to -35 kPa) at anthesis. The results showed that the grain yield and grain weight significantly decreased under SD in Zhefu802, but not in its NIL variety. SD also decreased expression of photosynthesis-related proteins in grains of Zhefu802, which resulted in its poorer drought resistance. Furthermore, the decreased grain filling rate rather than the grain size explained the observed decreased grain weight and grain yield under SD. Interestingly, these reductions were reversed by SA. Expression of proteins involved in glycolysis/TCA circle, starch and sucrose metabolism, antioxidation and detoxication, oxidative phosphorylation, transcription, translation, and signal transduction, were significantly down-regulated under SD and were significantly up-regulated in response to SA. The expression of these proteins was examined at transcriptional level and similar results were obtained. Inhibited expression of these proteins and related pathways contributed to the observed decrease in the grain filling rate of Zhefu802, and application of SA up-regulated expression of these proteins to improve grain weight. The findings of this study provide new insights into grain filling regulation by SA, and offer the scientific foundation for cultivation practice. Arsenic contamination has attracted worldwide concerns, owing to its toxicity and severe threat to human and environment. It is urgent to develop efficient adsorbents to remove arsenic pollutants. Within this paper, both pristine MIL-88A(Fe) and MIL-88A(Fe) decorated on cotton fibers were successfully fabricated using an eco-friendly method. The pristine MIL-88A(Fe) displayed outstanding adsorption performances towards four selected arsenic pollutants, in which the adsorption capacities toward As(III), As(V), ROX and ASA were 126.5, 164.0, 261.4 and 427.5 mg g-1, respectively. Additionally, MIL-88A(Fe) exhibited excellent removal efficiencies in a wide pH range and with the presence of different co-existing ions. It was proposed that the coordinative interactions of As-O-Fe between arsenic pollutants and MIL-88A(Fe) contributed to the superior adsorption performances. Furthermore, two MIL-88A(Fe)/cotton fibers composites were synthesized by both post synthesis (MC-1) and in-situ synthesis (MC-2), which demonstrated identically outstanding adsorption activities toward four selected arsenic pollutants. MC-1 and MC-2 enhanced the stability and reusability of MIL-88A(Fe), which was challenging issues of pristine MIL-88A(Fe) powder. Additionally, the fixed-bed column packed by MC-1 or MC-2 can continuously eliminate arsenic pollutants from the water flow. Coleonol nmr This work provided a new possibility of metal-organic frameworks to accomplish potentially large-scale application to purify the arsenic-contaminated water. Bisphenol A (BPA), a well-known endocrine disruptor, was reported to promote migration and invasion of lung cancer cells, but findings in human study is absent. A case-control study in Chinese population was conducted to evaluate the association between BPA exposure and non-small cell lung cancer (NSCLC), and explore the interaction between BPA exposure and estrogen-related genetic polymorphism on NSCLC. BPA concentrations were measured in urine samples using an UHPLC-MS method and rs2046210 in estrogen receptor α (ESR1) gene was genotyped by TaqMan genotyping system. Logistic regression was performed to estimate odds ratios (OR) and 95% confidence intervals (95% CI) for the association analyses. As a result, 615 NSCLC cases and 615 healthy controls were enrolled from Wuhan, central China. The mean age was 58.0 (SD 7.9) years old for controls and 59.2 (SD 8.8) years old for cancer cases. The creatinine-adjusted BPA levels were significantly higher in NSCLC cases than that in healthy controls (median 0.97 vs 0.73 μg/L, P  less then  0.001). Exposure to high levels of BPA was significantly associated with NSCLC (adjusted OR = 1.91, 95%CI 1.39-2.62, P  less then  0.001 for the highest quartile). We also observed a shallow concave dose-response relationship about the overall association between BPA and NSCLC. Moreover, interaction analyses showed that BPA exposure interacted multiplicatively with rs2046210, with a marginal P value (P = 0.049), to contribute to NSCLC. In conclusion, exposure to high levels BPA may be associated with NSCLC and the relationship may be modified by genetic polymorphism in ESR1. Perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS) are typical per- and poly-fluorinated alkyl substances (PFASs) that epidemiological studies have already associated with diabetes. However, insufficient data on their toxicity have been reported to explain any mechanism of action, which could justify such an association. Meanwhile, short-chain PFASs designed to substitute PFOA and PFOS, have already raised increasing concerns for their biosafety. Here, we evaluated whether common PFASs affected pancreatic and endocrine cell development using a human pluripotent stem cell pancreatic induction model and human pancreatic progenitor cell (hPP) endocrine induction model. The short-chain PFASs, pentafluorobenzoic acid, perfluorohexanoic acid, perfluorobutanesulfonic acid, and perfluorohexanesulfonic acid, homologous to PFOA or PFOS, did not significantly disrupt hPP generation, unlike PFOA and PFOS, based on pancreatic and duodenal homeobox 1 (PDX1) expression. However, SRY box 9 (SOX9) expression was suppressed in PDX1+ hPPs. All six PFASs did not disrupt SOX9 expression or hPP proliferation. However, endocrine differentiation of hPPs was affected, as indicated by neurogenin-3 (NGN3) downregulation, owing to abnormal increases in SOX9 and hairy and enhancer of split-1 (HES1) expressions. Thus, hyperactivation of NOTCH signaling was repressed after hPPs committed to the endocrine lineage. link2 In conclusion, our study demonstrates how powerful human pluripotent stem cell-based pancreatic differentiation models can be in developmental toxicity evaluations, compared to traditional toxicity assays, mostly based on live animals. Moreover, our findings suggest that PFASs may alter pancreatic development after the pancreatic domain emerges from the gut tube, and provide insights into their toxicity mechanisms. The remediation of cadmium-pyrene co-contaminated soil by electrokinetic (EK) and the influence factors were investigated in this study. The artificial contaminated soils were treated for 20 days in EK experimental setups without electrolyte solution reservoirs, to simulate in-situ remediation of unsaturated soil. The results indicated that polarity-reversing electric field had maintained soil pH in the range of 7.27-7.67. Cadmium (Cd) contaminant would aggregate near electrodes, and the average Cd concentration in these areas had reached 72.21 mg/kg (original 51.6 mg/kg), while the value in soil farthest away from electrodes was 33.58 mg/kg. The reasons for Cd aggregated were the insoluble hydroxide formations attribute to the frequently alternation of acid-base environment, and the decrease of pH and water holding capacity in soil away from electrodes would promote the dissolved Cd movement by electro-osmosis flow. Although the applied electric field could promote the growth and activity of pyrene-degrading microorganisms (PDM), the soluble Cd would be the restriction factor, especially in soil near electrodes. link3 However, the highest (56.38%) pyrene removal efficiency (PRE) was achieved near electrodes due to the synergistic effect of electric filed and PDM, and PRE was positively correlated with the PDM number in soil away from electrodes. The increasing application of Quantum Dots (QDs) is cause of concern for the potential negative effects for the ecosystem, especially in soils that may act as a sink. In this study, soil leaching experiments were performed in quartz sand packed columns to investigate the behavior of core-shell CdSe/ZnS QDs coated with either small ligands (TGA-QDs) or more complex polymers (POAMA-QDs). Fluorescence emission was compared to mass spectrometric measurements to assess the nanoparticles (NPs) state in both the leachate (transported species) and porous media (deposited amounts). Although both QDs were strongly retained in the column, large differences were observed depending on their capping ligand stability. Specifically, for TGA-QDs elution was negligible and the retained fraction accumulated in the top-columns. Furthermore, 74% of the NPs were degraded and 38% of the Se was found in the leachate in non-NPs state. Conversely, POAMA-QDs were recovered to a larger extent (78.1%), and displayed a higher transport along the soil profile. Further experiments with altered NPs showed that homo-aggregation of the QDs prior injection determined a reduced mobility but no significant changes in their stability. Eventually, ageing of the NPs in the column (15 days) caused the disruption of up to 92% of the original QDs and the immobilization of NPs and metals. These results indicate that QDs will accumulate in top-soils, where transformations phenomena will determine the overall transport, persistency and degradation of these chemicals. Once accumulated, they may act as a source for potentially toxic Cd and Se metal species displaying enhanced mobility.