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For O1/Campos, however, the presence of antibodies against epitopes different from those of the whole capsid, elicited by the presence of 12S particles in the vaccine, hampered the correlation between LPBE and VNT, which was improved by using purified O1/Campos 146S-particles for the liquid-phase of the LPBE. Interestingly, 146S particles but not 12S were efficiently bound to the ELISA plates, confirming the efficiency of the IE to detect antibodies against exposed epitopes. Our results indicate that any serological test assessing total antibodies or IgG1 against epitopes exposed in intact 146S-particles correlate with the levels of serum neutralizing antibodies in vaccinated pigs, and might potentially replace the VNT, upon validation. We recommend that antigen used for serological assays aimed to measure protective antibodies against FMDV should be controlled to ensure the preservation of 146S viral particles.BACKGROUND Creating National Sanitary City (CNSC) promotes appearance, environment sanitation and public health including vector management of cities in China. However, vector management especially mosquito management and the related administrative productivity of Patriotic Health Campaign System (PHCS) of National Sanitary Cities (NSCs) were questioned by many pest control professionals and citizens. In this study, we studied mosquito management of NSCs taking Wuhan as an example. The study aimed to (1) determine the distribution and abundance of immature mosquito habitats in built-up areas of Wuhan and (2) better understand the related administration procedure in CNSC. METHODS Immature mosquito habitat surveillance was carried out in randomly selected premises of driving schools (DSs), schools or kindergartens (SKs), property management residential areas (PMRAs), construction sites (CSs), wide roads with storm drains (WRSDs) and urban creeks (UCs) in built-up areas of Wuhan from July to October 2015 followetrict adherence to NSC standards. Further research on sustained promotion of urban mosquito management of NSCs, which focus on effective administration, as well as on improvement of related sectors of NSC standards should be carried out.A floating power generation device is designed and fabricated to overcome the power supply limitations of wireless sensor networks for environmental monitoring. Once there is a temperature difference between the upper surface exposed to sunlight and the lower surface in the water, the device is capable of generating power while floating in the wetland environment. Fresnel lenses were applied to concentrate solar irradiation on a selective absorbing coat. Meanwhile two vertical axis rotors were used to cool the cold side of the thermoelectric power generator by catching the breeze. The effects of solar irradiation, temperature distribution, load resistance, wind speed, the maximum power and the electrical efficiency of the thermoelectric power generator were analyzed. When subjected to solar irradiation of 896.38 W/m2, the device generated a potential difference of 381.03 mV and a power output of 8.86 mW via thermoelectric generation. In addition, compared with the system without wind, the output power was increased by approximately 10.96% in our system. The low power wireless networks, used in wetland environments, could be operated by the thermoelectric power generated by the floating device. Besides, this system offers powering solution for self-power miniature devices that are applied in aqueous environment.To make better use of global germplasm resources for improving the eating quality of hybrid rice, using the resequencing data from the 3,000 rice genomes project (3K RGP), the allelic variations of the rice Wx locus were analysed. WP1066 mouse With the exception of five rare alleles discovered for the first time in our study, most of these alleles were known alleles of Wx. Furthermore, a set of Kompetitive allele-specific PCR (KASP) markers based on these Wx alleles have been developed, and thirty-six main parents of hybrid rice from 1976 to 2018 were selected for Wx genotyping. The results showed that only three Wx alleles existed in the main parents of hybrids, and the allelic combination of the hybrids changed from Wxa/Wxb and Wxlv/Wxb to Wxb/Wxb with the development of hybrid rice. Wxb is widely used in the male parents of hybrid rice. Wxa and Wxlv were used in the female parents of early hybrid rice, and they were gradually replaced by Wxb. In the future, more favourable Wx alleles from cultivated rice should be identified, introduced, and effectively used to improve hybrid rice quality.The nonlinear dynamics of a bird-like flapping wing robot under randomly uncertain disturbances was studied in this study. The bird-like flapping wing robot was first simplified into a two-rod model with a spring connection. Then, the dynamic model of the robot under randomly uncertain disturbances was established according to the principle of moment equilibrium, and the disturbances were modeled in the form of bounded noise. Next, the energy model of the robot was established. Finally, numerical simulations and experiments were carried out based on the above models. The results show that the robot is more likely to deviate from its normal trajectory when the randomly uncertain disturbances are applied in a chaotic state than in a periodic state. With the increase of the spring stiffness under the randomly uncertain disturbances, the robot has a stronger ability to reject the disturbances. The mass center of the robot is vital to realize stable flights. The greater the amplitude of randomly uncertain disturbances, the more likely it is for the robot to be in a divergent state.Aiming at the problem of fast certification for a constrained iris in the same category caused by the unstable iris features caused by the change of the iris acquisition environment and shooting status under lightweight training samples, a one-to-one fast certification algorithm for constrained unsteady-state iris based on the scale change stable feature and multi-algorithm voting is proposed. Scale change stable features are found by constructing an isometric differential Gaussian space, and a local binary pattern algorithm with extended statistics (ES-LBP), the Haar wavelet with over threshold detection and the Gabor filter algorithm with immune particle swarm optimization (IPSO) are used to represent the stable features as binary feature codes. Iris certification is performed by the Hamming distance. According to the certification results of three algorithms, the final result is obtained by multi-algorithm voting. Experiments with the JLU and CASIA iris libraries under the iris prerequisite conditions show that the correct recognition rate of this algorithm can reach a high level of 98% or more, indicating that this algorithm can improve the operation speed, accuracy and robustness of certification.

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