Allredtychsen0488

Transmission electron microscopy (TEM) showed the existence of nanocellulose with an average aspect ratio of 10.45 ± 3.44. In the future, dunchi fiber has a potential to be used as a renewable source to produce cellulose and subsequently its nanocellulose for a wide range of applications in composite materials. Development of drug delivery systems has emerged out as significant field in medicinal chemistry because of their localized action, low frequency of drug administration and sustained release of drug at the site of action for a prolonged time. Out of various reported methods, in the present work, we report use of five different polysaccharides for the development of sustained release systems for curcumin, utilizing a surfactant, cetyltrimethylammonium bromide (CTAB). Four of these were novel systems and were first optimized. Sustained release of optimized supports was studied by anti-oxidant, serum protein binding and anti-cathepsin activities. Particle size, FT-IR and SEM were used to characterize the modified supports. CTAB-modified-NCCS, -pectin-15 and Alg-5 were found to be the best supports as they released appreciable amount of curcumin for a longer time. https://www.selleckchem.com/products/benzylpenicillin-potassium.html The results have also been interpreted using chemical modeling studies. The objective of the study was to explore the formation mechanism and thermodynamic properties of chitosan (CS)-potato protein isolate (PPI) complex under DHPM treatment. The transmission electron microscopic (TEM) results showed the formation of a complex between CS and PPI. Meanwhile, particle size and zeta-potential were shown to increase with increasing CS concentration, further confirming the formation of the complex. The surface hydrophobicity results showed CS was bound to PPI by hydrogen bond. The ultraviolet and fluorescence spectral analysis exhibited CS formed a protective mechanism against PPI destruction, preventing the exposure of tyrosine and tryptophan residues. Infrared spectrum and circular dichroism spectral analysis revealed no occurrence of chemical reaction between CS and PPI under DHPM treatment, further indicating that they are bound by hydrogen bond and hydrophobic interaction. Moreover, CS addition was shown to enhance the intermolecular interaction and promote the formation of intermolecular hydrogen bond network. Differential scanning calorimetry (DSC) and thermo gravimetric analysis (TGA) revealed that CS addition could improve the thermal stability of PPI. These results have shed light on the formation mechanism and thermodynamic properties of the CS/PPI complex and facilitate its application in food industry. V.Modified arrowroot starch was investigated as a fat replacer in mayonnaise. Arrowroot starch was modified by octenyl succinic anhydride (OSA), annealing (ANN), citric acid hydrolysis (CA), acetylation (ACT) and heat-moisture treatment (HMT). The different starch pastes were used to replace mayonnaise fat at levels of 30% and 50%. Color, viscoelastic properties, and emulsion stability of the fat-reduced mayonnaises and full-fat (FF) version were evaluated, according to the type of modified starch and fat replacement ratio. Physicochemical, thermal, and pasting properties of all starch types differed due to the modification method. Shear stress of mayonnaise was fitted to the Casson and Herschel-Bulkley model, respectively. As partial fat replacers, ANN-modified starch and OSA-starch at 30%, and CA-starch at 30% and 50% showed high yield stress. The elastic moduli (G') of fat-reduced mayonnaises were lower than FF, but mayonnaise with ANN, OSA, and CA showed higher G' than other modified starches. Fat-reduced mayonnaises displayed higher emulsion stability than the FF, especially those with ANN-modified starch, OSA-starch, and CA-starch. In principal component (PC) analysis, groups with high and low emulsion stability were divided by PC1. Overall, ANN-, OSA-, and CA-modified starches were identified as suitable fat replacers in mayonnaise. In this study, a low-molecular-weight saccharide fragment (LMWAs-L) was prepared from alginate lyase (EC 4.2.2.3) hydrolyzed ascophyllan by ultra-filtration separation method. LMWAs-L was a homogeneous saccharide fraction with an average molecular weight of 6.96 kDa. Enzymolysis process optimization experiments revealed that the optimum process parameters for preparing LMWAs-L were the enzyme concentration 0.02 U/mL, initial pH 6.8, and enzymolysis temperature 43 °C. After optimization, the yield of LMWAs-L was increased to 9.74% higher than that without optimization. Interestingly, LMWAs-L exhibited stronger enhancing activities on the proliferation and migration of human skin fibroblasts cells in vitro and better antibacterial activities as compared to native ascophyllan at the same mass concentration. Our study establishes a simple way to prepare low-molecular-weight saccharide with beneficial bioactivities from ascophyllan efficiently. This is the first report to reveal that ascophyllan and its low-molecular-weight saccharide have the potentials to be developed as natural biological dressing and antibacterial agents. Sulphated Polysaccharides (SP) were extracted from a brown seaweed Sargassum swartzii by two extraction methods using hydrochloric acid and hot water. The sulphated polysaccharide yield using the hot water extraction method was found to be higher and hence used for further study. The extracted polysaccharide was characterized using UV, FT-IR, biochemical and thin layer chromatography analyses. Further, the purity of the extracted polysaccharide was ascertained by HPLC analysis. The sugars present in the sulphated polysaccharide were revealed by acid hydrolysis. The structure of the extracted SP was revealed as fucoidan using the NMR spectrum. Thermal stability of the sulphated polysaccharide was assessed using Thermogravimetric analysis and polymer was found to be stable up to 700 °C. Anti-oxidant and anti-inflammatory activities were evaluated using phosphomolybdenum and BSA assay, respectively. Cell proliferation analysis using MTT assay against normal cell lines revealed that the polysaccharide is biocompatible while with cancer cell lines, the compound exhibited potential anti-proliferative activity.