Albrechtsenkrag2465

Cinnamon is a popular spice used in food products. Its flavor varies by its chemical profile. Cinnamon flavoring powder is a unique form of material with essential oil encapsulated in wall material, which improves the stability and homogeneity but also increases the difficulties for analysis. A specific and rapid method is needed to analyze the main components for its quality and safety.

An analytical method for the quantification of cinnamon flavoring powder was developed and validated. The characteristic components for analysis were selected as coumarin, trans-cinnamic acid, trans-cinnamaldehyde, and eugenol.

This quantitation method with ultra-performance liquid chromatography coupled with diode array detector analysis was achieved by material extraction followed by chromatographic separation on C18 columns eluted with a gradient acetonitrile-water mobile phase. The detected wavelength was determined as 280 nm.

Linear regression of calibration curves for each component was validated (R2 > 0.9995). read more The specificity, LOD and LOQ, precision, accuracy, and ruggedness of the developed method were also evaluated.

Such an approach is applicable for the simultaneous determination of these four characteristic constituents in cinnamon flavoring powder used in manufacturing and quality control of nutritional products.

This study describes the selection of four components for analysis, the efficient extraction of them from cinnamon flavoring powder, and the rapid quantitation of these four characteristic components in these materials.

This study describes the selection of four components for analysis, the efficient extraction of them from cinnamon flavoring powder, and the rapid quantitation of these four characteristic components in these materials.

The Enterobacteriaceae and generic Escherichia coli are routinely enumerated in foods as part of product release criteria, or in the case of swabs, for environmental monitoring.

Microbiologique Microfilm™ EBEc is intended to provide a rapid and easy-to-use method for simultaneous enumeration of Enterobacteriaceae and E. coli on foods and environmental surfaces. Methods This study evaluated the performance of Microfilm™ EBEc against ISO methods (ISO 21528-22017 for Enterobacteriaceae and ISO 16649-2 2001 for E. coli) in 20 food matrixes and two environmental surfaces. Inclusivity, exclusivity, lot-to-lot reproducibility, ruggedness and stability studies were also performed on Microfilm™ EBEc.

No significant differences and high correlation coefficients (R2) were observed between the Microfilm™ EBEc and the corresponding ISO methods in spiked food matrixes and environmental samples. Inclusivity studies showed expected results for all the E. coli and Enterobacteriaceae strains tested. In terms of exclusivirresponding ISO methods for enumeration of Enterobacteriaceae and E. coli. Highlights Microfilm™ EBEc offers a convenient and relatively fast test method for simultaneous enumeration of Enterobacteriaceae and E. coli in 24 h and has an advantage over the corresponding ISO methods that require two assays on the same sample for enumeration of Enterobacteriaceae and E. coli Gram-negative indicator groups.

The emulsion induced by emulsion breaking (EIEB) procedure was previously reported for the extraction of copper, iron, manganese, and nickel from liquid oil samples such as vegetable oil.

To optimize the EIEB procedure for determination of copper, iron, manganese, and nickel in solid oil (margarine) samples by Graphite Furnace Atomic Absorption Spectrometry (GFAAS).

The extraction procedure uses a surfactant in nitric acid to form an emulsion followed by heating to break the emulsion. Optimization included variation of the test portion size, the type and concentration of the surfactant, the concentration of nitric acid in the aqueous solution, the emulsion agitation time, heating temperature, and the time required to break the emulsion.

Mean element concentrations of 11 margarine samples were in the following ranges Cu 0.031-0.131 µg/g, Fe 5.7-24.9 µg/g, Mn 0.542-1.11 µg/g, and Ni 0.108-0.134 µg/g. Under the optimized extraction conditions, the detection limits (µg/kg) were 4.8, 13, 1.5, and 23 for Cu, Fe, Mn, and Ni, respectively. The accuracy of the extraction procedure was determined by comparison to commonly used microwave digestion procedure. The EIEB results were not statistically different from the microwave digestion results when analyzed by GFAAS as determined by the statistical tests.

The EIEB procedure was shown to be equivalent to the commonly used microwave digestion procedure for extraction of analytes from margarine samples.

The optimized EIEB extraction procedure is simple, rapid, low cost, and environmentally friendly. It has improved detection limits and allows calibration with aqueous standards.

The optimized EIEB extraction procedure is simple, rapid, low cost, and environmentally friendly. It has improved detection limits and allows calibration with aqueous standards.

Actero™ Salmonella Enrichment Media1 (Actero™ Salmonella) is a culture broth developed to recover Salmonella spp. from foods and environmental surfaces. Performance of Actero™ Salmonella broth has already been assessed and validated (AOAC Performance Tested MethodSM 041403) for the detection of Salmonella spp. in various foods, feeds and environmental samples.

This study aimed to validate the performance of a modified version of Actero™ Salmonella broth by incorporating one of the two liquid supplements into the powdered formula.

Inclusivity, exclusivity, stability, and lot-to-lot studies were carried out. Raw ground beef, chicken carcass rinse, dry pet food and stainless steel samples were enriched for 14-20 h at 35-39°C and analyzed using real-time PCR assay as well as by direct plating.

The Probability of Detection assay confirmed the equivalent performance of the alternative methods as compared to the reference methods. All Salmonella strains, except Salmonella II 57 z29-, were able to grow in Actero™ Salmonella broth. One-half of the non-target strains did not grow in Actero™ Salmonella broth, whereas the atypical for Salmonella growth was observed for other non-target microorganisms subsequently plated onto selective and differential agars. Lot-to-lot consistency was demonstrated for three consecutively manufactured lots of the broth. The liquid broth was proven to be stable at 4°C for up to 9 weeks of storage.

The incorporation of one of the two specific supplements into a powdered formula of Actero™ Salmonella broth made it more convenient to use without compromising the performance and accuracy.

The incorporation of one of the two specific supplements into a powdered formula of Actero™ Salmonella broth made it more convenient to use without compromising the performance and accuracy.

The glycopeptide antibiotics are a class of antimicrobial drugs that are an important alternative for cases of bacterial infections resistant to penicillins, besides being able to be used to treat infections in people allergic to pencilin. They have great activity against Gram-positive microorganisms, including methicillin-resistant Staphylococcus aureus (MRSA), by inhibiting the cell wall synthesis.

There are many analytical methods in the literature for determination of antimicrobial glycopeptide vancomycin in different matrixes that are very effective; however, all of them use toxic solvents, contributing to the generation of waste, causing damage to the environment and to the operator, as well as increased costs of analysis.

The most prevailing method found was high performance liquid chromatography (HPLC), followed by microbiological assays and, in less quantity, spectrometric methods. The chromatographic methods use organic solvents that are toxic, such as acetonitrile and methanol, and buffer solutions, that can damage the equipment and the column. In the microbiological assays the disc diffusion methods are still in the majority. The spectrophotometric methods were based in the UV-Vis region using buffer solutions as a diluent.

All these methods can become greener, following green analytical chemistry principles, which could bring benefits both to the environment and the operator, and reduce costs.

In this paper, a literature review regarding analytical methods for determination of vancomycin was carried out with a suggestion of greener alternatives.

In this paper, a literature review regarding analytical methods for determination of vancomycin was carried out with a suggestion of greener alternatives.

The goal of this work was to establish a method to identify and quantify the main active components in Angelicae Pubescentis Radix (APR) quickly, simply, and accurately. This paper reports a novel method which can determine osthol, isoimperatorin, and columbianadin using 1H-qNMR simultaneously and quantitatively.

In comprehensive consideration of resolution of target signals and the solubility of materials, dimethyl sulfoxide-d6 (DMSO-d6) was selected as an optimal 1H-qNMR solvent and pyrazine was used as internal standard substance (δ8.66 ppm). The quantitative peaks of three active components were determined using specific 1H resonances at δ7.54-7.56 ppm for osthol, δ6.83-6.85 ppm for columbianadin, and δ6.31-6.32 ppm for isoimperatorin.

The results show that the method has good precision, stability, and repeatability. The content of APR plant material from Huating is 9.8 mg/g, 5.6 mg/g, and 15.6 mg/g for osthol, columbianadin, and isoimperatorin, respectively. Furthermore, the experimental process is simple and the test time is short (1 min).

The proposed quantitative 1H-qNMR methodology can be used for the quality control of APR.

The proposed quantitative 1H-qNMR methodology can be used for the quality control of APR.A flavoring agent is a compound that serves to add flavor with a pleasant scent and is used as a feed additive. Current flavor analysis methods include reflux pretreatment, titration, neutralization titration, and inversion; these are all analytical methods in which deviations and errors between experiments are generated. Titration methods are characterized by difficult selectivity analysis both for mixtures containing two or more types of flavoring agents and also for very low content samples. Current analysis methods are therefore particularly unsuitable for these sample types. Thus, more precise and accurate analysis of flavor agents is needed. This study intends to develop and verify a multi-component simultaneous analysis method that can accurately confirm the guaranteed content of 12 flavor agents of supplementary feeds distributed in the market, the goal being to establish a universally trusted method. Method validation was performed according to the International Conference on Harmonization (ICH) and International Union of Pure and Applied Chemistry (IUPAC) guidelines. Method validation was performed in terms of linearity, sensitivity, selectivity, accuracy, and precision. The limits of detection (LOD) for the instrument employed in these experiments ranged from 0.44-4.77 mg/kg, and the limits of quantification (LOQ) ranged from 1.32-14.31 mg/kg. Average recoveries of the 12 flavoring agents ranged from 75.1-111.4%. Maximum %RSD values for intraday and interday peak area variation are 13.09% and 13.08%, respectively. A novel and simple method for detecting 12 flavoring agents in animal feed supplements using a gas chromatography-flame ionization detector (GC-FID) was developed.