Aguilarrytter9492

Herein, we developed a rapid method for detection of genetically modified soybean (GTS 40-3-2) products using loop-mediated isothermal amplification (LAMP). A crude 5-minute extraction method was established for DNA extraction from soybeans and soybean products. LAMP reaction for CaMV35S promoter was optimized and the fastest threshold time (Tt) was 14 min with 4 mM magnesium ions at 63 °C. A portable instrument was designed to perform real-time LAMP in the field. As little as 0.1% GM soybean, specifically, or 0.5% GM ingredients in Chinese traditional tofu could be detected in 30 min from sampling to results. We used this method to further assess other five soybean products to determine whether they contained transgenic ingredients and compared the results with those obtained using PCR, which suggested the proposed method was applicable for rapid detection of genetically modified soybean in food products. Herein, a new type of coordination polymer networks (CPNs), where both of 2,6-Pyridinedicarboxylic acid (DPA) and guanylate monophosphate (GMP) chelate with Eu3+, are firstly synthesized (GMP/Eu/DPA). After mixing with GMP/Tb CPNs, a novel ratiometric dual lanthanide nanoprobe is constructed. Alkaline phosphohydrolase (ALP) specifically induces the cleavage of phosphate ester group in GMP. Therefore, the addition of ALP causes the fluorescence quenching of GMP/Tb, and then the emissions of GMP/Eu/DPA enhance as the result of the formation of Eu/DPA complexes. Glyphosate, as an organophosphorus pesticide, can vehemently inhibit the catalytic activity of ALP, so a ratiometric detection of glyphosate can be achieved with a linear range from 0.015 to 8.45 μg/mL. The present strategy also shows good recoveries for measuring glyphosate in rice, millet, soybean, milk, tap water, and mountain spring water, suggesting a great potential for applications in foods. A simple, biocompatible and an enzyme-free sensing platform was developed for detection of paraoxon. The surface of a glassy carbon electrode was modified with an electrodeposition of stearic acid/nanosilver composite at -0.7 V for 40 s. The paraoxon undergoes electro-reduction at -550 mV on the modified electrode, and the limits of detection (LOD) was calculated as 0.1 nM (S/N = 3) using differential pulse voltammetry which is lower than that of the existing materials reported. The high stability observed with the modified electrode for prolonging period indicated that the sensitivity of the electrode remains active for several runs of the analysis. The developed analytical strategy was implemented for onion and paddy grain samples and good recovery rates were observed. Also, it was applied for analyzing the purity of the commercial paraoxon sample. The reliability of the developed strategy was confirmed by comparing the results of electrochemical approach with that of HPLC technique. The current study explores the first full mode liquid microextraction technique coupled with surface-enhanced Raman spectroscopy (SERS), and has been successfully applied for chromium speciation in food and environmental matrices. Herein, chromium as chlorochromate anion [CrO3Cl]- and the cationic rhodamine 6G [RG]+ dye has been extracted in organic phase as a complex ion associate [RG+.CrO3Cl-.nS]org at pH ≤ 1.0. Afterwards, the extracted phase was deposited on the surface of the nano-flower shaped silver nanoparticles substrate and the SERS response was monitored against the reagent blank at 1505 cm-1. Substrate characterizations, reaction mechanism assignment, stoichiometry, speciation, analytical applications, selectivity and validation were performed. The analytical procedure exhibits a detection limit of 0.03 µg L-1 under the optimized experimental conditions. The accuracy of the proposed strategy was validated by inductively coupled plasma optical emission spectrometry method using student's t- and F tests at 95% confidence. Hydroxytyrosol (HT) possesses significant biological activity. However, the methodologies for its quantification always suffered from low sensitivity, intricate treatment and high sample consumption. Here, we presented the very first attempt for specific extraction of HT through cis-diol recognition mechanism. By using easily prepared zirconia as dispersive solid phase extraction medium, HT from small amount of wine (10 μL), oil (20 mg) and plasma (100 μL) was efficiently purified within ten minutes. Coupled with LC-MS/MS analysis, the method limit of detection (LOD) could reach 1 ng/mL in wine, 0.5 μg/kg in oil and 0.1 ng/mL in plasma. Profited by this superior method, HT analysis was successfully performed in diverse wine and oil products as well as human plasma samples after intake of extra virgin olive oil. In addition, we further confirmed the endogenous HT was undetectable from routine human plasma even after upgrading the detection sensitivity through post isonicotinoyl chloride derivatization. The network of the Maillard reaction can be influenced by the presence of polyphenols. In this paper, we evaluated the ability of secoiridoids to interact with asparagine and lysine tuning the formation of dietary advanced glycation end-products (d-AGEs), dicarbonyls and acrylamide. Olive oil mill wastewater polyphenol powders (OMWP) were added to glucose and lysine or asparagine in silica model systems to mimic water activity present in cookies. Results revealed that acrylamide, Amadori compounds and N-ε-carboxyethyllysine (CEL) were reduced to 50%, after 13 min at 180 °C; for the reduction of N-ε-carboxymethyllysine (CML), secoiridoids were effective only in model systems with the addition of acacia fiber and maltodextrin as coating agents. In cookies, OMWP at three different concentrations decreased the concentration of protein bound Amadori compounds, CML, CEL and dicarbonyls. Acrylamide and 5-hydroxymethylfurfural were reduced to 60% and 76% respectively, highlighting the ability of secoiridoids-based functional ingredients in controlling d-AGEs formation. Interactions between bile acids and plant-based materials, and the related feedback mechanisms in enterohepatic circulation, have been considered targets for lowering cholesterol. This study aimed to identify lupin compounds that interact with primary bile acids on molecular level. Lupin cotyledons were fractionated and bile acid adsorbing activities were investigated using in vitro digestion, equilibrium dialysis and kinetic analyses. Protein- and fibre-enriched fractions significantly (p ≤ 0.05) adsorbed chenodesoxycholic acids (up to 2.33 µmol/100 g DM). Alcohol purification showed that bile acid adsorption is independent of protein and fibre structures. selleck inhibitor Moreover, high adsorption was observed with an alcohol extract (6.97 µmol chenodesoxycholic acids/100 g DM) that was rich in phytochemicals, such as flavonoids (1842 mg/100 g DM). These results suggest the formation of hydrophobic interactions between polyphenols and bile acids. Further studies of molecular mechanisms are required to define the contributions of polyphenols to the cholesterol-lowering actions of lupins.