Acevedodyhr3098

Southern hybridization confirmed the integration of the hptII gene into the genome of C. sorokiniana. The qRT-PCR and Western blot analyses confirmed hptII and GUS gene expression in the transgenic cell lines. The specific growth rate, biomass doubling time, PSII activity, and fatty-acid profile of transformed cells were found similar to wild-type untransformed cells, clearly indicating the growth and basic metabolic processes not compromised by transgene expression. This protocol can facilitate opportunities for future production of biofuel, carotenoids, nutraceuticals, and therapeutic proteins.

The online version contains supplementary material available at 10.1007/s13205-021-02750-7.

The online version contains supplementary material available at 10.1007/s13205-021-02750-7.The current study illustrates the growth kinetics of an efficient PAH and heterocyclic PAH degrading bacterial strain, Pseudomonas aeruginosa RS1 on fluorene (FLU) and dibenzothiophene (DBT) over the concentration 25-500 mg L-1 and their concomitant degradation kinetics. The specific growth rate (µ) was found to lie within the range of 0.32-0.57 day-1 for FLU and 0.24-0.45 day-1 for DBT. The specific substrate utilization rate (q) of FLU and DBT over the log growth phase was between 0.01 and 0.14 mg FLU mg VSS-1 day-1 for FLU and between 0.01 and 0.18 mg DBT mg VSS-1 day-1 for DBT, respectively. The µ and q values varied within a narrow range for both FLU and DBT and they did not follow any specific trend. Dissolution together with direct interfacial uptake was the possible uptake mechanism for both FLU and DBT. The q values over the log growth phase depicts the specific substrate transformation rates. Kirby-Bauer disc diffusion studies performed using an E. coli strain indicated accumulation of some toxic intermediates of FLU and DBT during their degradation. Decrease in TOC and toxicity towards the end of the degradation experiments indicates further utilization of the intermediates.

The online version contains supplementary material available at 10.1007/s13205-021-02742-7.

The online version contains supplementary material available at 10.1007/s13205-021-02742-7.The growth and development of watermelon and melon are affected by abiotic stresses such as cold, salinity and drought. Plant superoxide dismutase (SOD) proteins exerted great effects on plant growth, development and response to abiotic stresses. However, little is known about the characteristics of watermelon and melon SOD gene families and their expression patterns under abiotic stresses. In this study, the genome-wide identification of SOD genes and their expression patterns under abiotic stresses has been done in watermelon and melon. RCM-1 manufacturer Seven SODs were identified in watermelon and melon, respectively. Chromosome location indicated that the SODs were dispersedly distributed on 4-6 chromosomes. Almost all the SOD proteins contained 300 amino acids or less and the intron numbers of SODs ranged from 5 to 7. On the basis of phylogenetic analysis, the SODs were classified into six sub-groups which was also verified by similar motif composition, gene structure and sub-cellular location. Gene ontology analysis displayed that many SOD proteins participated in binding, catalytic, antioxidant activity and stimulus-response. Cis-regulatory elements related to stresses and hormones were found in the promoters of the SODs. Based on the quantitative real-time PCR, most of CmSOD and ClSOD genes showed obvious up-regulation under low-temperature, NaCl and PEG6000 treatments. The abiotic stress-responsive SOD genes were identified to improve watermelon and melon tolerance against abiotic stresses. This was a preliminary study to describe the genome-wide analysis of SOD gene family in watermelon and melon, and the results would facilitate further study of gene cloning and functional verification of SOD genes response to abiotic stresses in watermelon and melon.

The online version contains supplementary material available at 10.1007/s13205-021-02726-7.

The online version contains supplementary material available at 10.1007/s13205-021-02726-7.This study aimed to identify a symbiotic fungus strain HX-1 with anti-Vibrio harveyi activity and isolate and identify the active compound. The HX-1 strain was identified as Aspergillus fumigatus according to the morphological characteristics and internal transcribed spacer (ITS) sequence analysis. The compound was isolated from the fermentation product of HX-1 strain through ethyl acetate extraction, silica gel and Sephadex LH-20 column chromatography, and semi-preparative HPLC techniques using an antibacterial-guided fractionation method. According to its physicochemical properties and spectral characteristics, the compound was identified as trypacidin having the same anti-V. harveyi activity as streptomycin sulfate, with the minimum inhibitory concentration of 31.25 µg/mL.Many fish species are known to feed on jellyfish. Herein, we observed the effects of jellyfish feeding on silver pomfret using gas chromatography tandem time-of-flight mass spectrometry (GC-TOF-MS) based on metabolomics. We studied the effects of feeding on jellyfish on skin and serum immune of silver pomfret. Healthy silver pomfret (initial weight, 13.40 ± 1.565 g) was divided into two groups control and feeding. The pomfrets were fed jellyfish at 2, 6, 12, 24, and 72 h, and samples were obtained. Statistical analysis revealed that after jellyfish feeding, most serum immune indicators did not show a significant change; however, skin immune indicators indicated that silver pomfret elicit a stress response on encountering jellyfish, gradually adapting to their presence. We therefore conducted further experiments involving two groups group A, which was not fed any extra jellyfish, and group B, which was fed extra jellyfish (approximately 10% weight of silver pomfret) every day for 60 days. Orthogonal partial least squares discriminant analysis led to the identification of stronger biomarkers, with the liver metabolome showing obvious variations between the groups (group B vs. A). After feeding jellyfish by silver pomfret, some amino acids, amines, and unsaturated fatty acids in the liver tissue showed a significant increase. Our results, thus, not only reveal changes in physiological indices of silver pomfret after feeding on jellyfish but also provide a new idea for further optimizing the feed formula for silver pomfret culture.