Aaruphobbs1149

Diagnosis of malignant biliary obstruction is complicated and lacks accuracy. Exosomes may be secreted by malignant tumors; intact miRNAs from exosomes might serve as potential biomarkers for the disease.

To identify exosomal microRNAs in human bile among benign and malignant biliary obstructions.

Bile samples were collected from patients undergoing therapeutic endoscopic retrograde cholangiopancreatography for biliary obstruction. Exosome microRNAs were determined by RNA-sequencing in the discovery cohort, which comprising benign (n = 5) cases and malignant biliary obstruction (n = 5) cases. Then, the diagnostic performance of the two up-regulated microRNAs (mir-483-5p and mir-126-3p) of bile exosomes was verified by analysis of 82 patients with a diagnosis of malignant (n=37) or nonmalignant (n=45) biliary obstruction.

In both cohorts, the expressions of mir-483-5p and mir-126-3p were significantly higher in bile exosomes samples from patients with malignant biliary obstructions than controls. In the verification cohort, the two miRNAs can distinguished the benign and malignant groups with high diagnostic accuracy and specificity; the diagnostic values of the two microRNAs were better than serum carbohydrate antigen 19-9 (CA19-9), area under the curve (AUC) were 0.81 and 0.74.

The expression of exosomal mir-483-5p and mir-126-3p in the bile samples discriminates between patients with malignant and nonmalignant biliary obstructions.

NCT03102268.

NCT03102268.The role of sex steroids in mammalian maturation is well established. Recently, it has been increasingly appreciated that sex steroids also play an important role in the propensity of adults to develop a myriad of diseases. The exposure and responsiveness of tissues to sex steroids varies among individuals and between the sexes, and this has been correlated with gender-specific differences in the composition of the intestinal microbiota and in susceptibility to metabolic, autoimmune, and neoplastic diseases. Here we focus on recent studies that demonstrate an interplay between sex steroids, the intestinal immune response, and the intestinal microbiota. While correlations between biological sex, the intestinal innate immune response, intestinal inflammation, and intestinal microbiota have been established, many gaps in our knowledge prevent the emergence of an overarching model for this complex interaction. Such a model could aid in the development of prebiotic, probiotic, or synthetic therapeutics that decrease the risk of autoimmune, metabolic, neoplastic, and infectious diseases of the intestine and mitigate the particular health risks faced by individuals receiving sex steroid treatment.Coffee pulp is one of the major underutilized byproduct of coffee processing in farm level. Disposal of this agro-industrial waste has become one of the most challenging tasks for coffee planters. However, most of the efforts are towards the management of coffee pulp as an effluent, and not-on re-use. The problem is compounded due to the large volumes produced in diluted forms, which makes it expensive to reuse. The preliminary proximate analysis of coffee pulp indicated it to be rich in pectin and polyphenols. The efficacy of various chemicals like ethanol, sulfuric acid, hydrochloric acid, nitric acid, ammonium oxalate and metal salts for effective precipitation of pectin from coffee pulp was evaluated. HPLC characterization of the extracted and concentrated polyphenols fractions was analyzed. The maximum extraction of pectin was achieved by using metal salts and ethanol with 6.0% and 6.7% on wet weight basis respectively. The equivalent weight of extracted pectin (1180.5 mg/g) was found to be higher than that of commercial pectin (724.8 mg/g). The methoxyl content of the commercial pectin and crude pectin were 9.3 and 5.6% respectively. Gallic, vanillin, catechin, ethyl catechol, coumaric, Caffeic, and ferulic acid were the major polyphenols as quantified by the HPLC. The polyphenol fraction showed a good antioxidant activity with phosphomolybdate, FRAP, DPPH, and ABTS radicals respectively. The sustainable utilization of coffee pulp as a source of pectin and polyphenols with good antioxidant activities could help to solve the problem of waste generated in coffee processing in farm level.This study explores how Korea's ELV recycling policy can be improved to meet the targeted ELV recycling rate by reviewing the characteristics of the recycling policies adopted in Korea and abroad and conducting an economic analysis of each step of the ELV recycling process, namely, dismantling (including waste gas treatment), shredding, and ASR recycling. The costs and revenues of each ELV treatment process was computed using the data from operators in Korea to estimate their economic feasibility. The findings indicated that, in the case of Korea, dismantling accounted for the largest portion of the ELV recycling process, pointing to the need for policies providing financial support to ELV dismantlers to enhance the overall ELV recycling rate. At the post-dismantling stage, it was found that greater attention to the ELV shredding stage and diversifying the treatment methods for recycled automotive shredder residue (ASR) can be valid measures to reduce Korea's current reliance on dismantling. click here However, institutional or financial support will be essential to assist with the initial investment costs for developing ASR treatment technologies and constructing new facilities.To optimize output streams in mechanical waste treatment plants dynamic particle size control is a promising approach. In addition to relevant actuators - such as an adjustable shredder gap width - this also requires technology for online and real-time measurements of the particle size distribution. The paper at hand presents a model in MATLAB® which extracts information about several geometric descriptors - such as diameters, lengths, areas, shape factors - from 2D images of individual particles taken by RGB cameras of pre-shredded, solid, mixed commercial waste and processes this data in a multivariate regression model using the Partial Least Squares Regression (PLSR) to predict the particle size class of each particle according to a drum screen. The investigated materials in this work are lightweight fraction, plastics, wood, paper-cardboard and residual fraction. The particle sizes are divided into classes defined by the screen cuts (in mm) 80, 60, 40, 20 and 10. The results show assignment reliability for certain materials of over 80%.