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We conclude the article by providing policy implications so that emissions trading policies can be integrated with the newly developed pollution permitting system. Green roofs are expanding internationally due to the well documented benefits they provide for buildings and cities. This requires transferable knowledge of the technological aspects influencing green roof design, particularly substrate properties. GPNA clinical trial However, this is made difficult due to differences in substrate testing methods referred to in green roof guidelines and standards. Therefore, we tested a green roof substrate using laboratory-based methods from European (FLL), North American (ASTM) and Australian (AS) green roof guidelines and standards to determine how these methods vary in characterising substrate physical properties (bulk density, water permeability and water holding capacity at field capacity (WHC)). Further, we compared the results from the laboratory-based methods with measures of bulk density and WHC in green roof platforms to determine whether standard methods accurately represent substrate properties in-situ. Results from the standard test methods varied due to differences in sample compaction. The standard test methods that employ Proctor hammer compaction (FLL and ASTM) had greater bulk density (at field capacity and dry) and lower water permeability than Australian standard methods that employ free-fall compaction. WHC did not differ among the standard methods. The Australian standard method better reflected bulk density at field capacity and WHC of the substrate under in-situ green roof conditions. For mineral based substrates, our results suggest that for the FLL and ASTM testing methods, a single Proctor hammer drop will produce a degree of sample compaction equivalent to the free-fall method (AS) and be more representative of bulk density in-situ. Subtle changes in testing procedures would allow for more direct comparison of substrate properties between standard methods and help enable the international transfer of knowledge for substrate design. The effects of exogenous Escherichia coli on nitrogen cycling (N-cycling) in freshwater remains unclear. Thus, seven ecosystems, six with submerged plants-Potamogeton crispus (PC) and Myriophyllum aquaticum (MA)-and one with no plants were set up. Habitats were assessed before and after E. coli addition (107 colony-forming units/mL). E. coli colonization of freshwater ecosystems had significant effects on bacterial community structure in plant surface biofilms and surface sediments (ANOVA, P  less then  0.05). It reduced the relative abundance of nitrosification bacteria (-70.94 ± 26.17%) and nitrifiers (-47.86 ± 23.68%) in biofilms which lead to significant reduction of ammoxidation in water (P  less then  0.05). The N-cycling intensity from PC systems was affected more strongly by E. coli than were MA systems. Furthermore, the coupling coefficient of exogenous E. coli to indigenous N-cycling bacteria in sediments (6.061, average connectivity degree) was significantly weaker than that in biofilms (9.852). Additionally, at the genus level, E. coli were most-closely associated with N-cycling bacteria such as Prosthecobacter, Hydrogenophaga, and Bacillus in sediments and biofilms according to co-occurrence bacterial network (Spearman). E. coli directly changed their abundance, so that the variability of species composition of N-cycling bacterial taxa was triggered, as well. Overall, exogenous E. coli repressed ammoxidation, but promoted ammonification and denitrification. Our results provided new insights into how pathogens influence the nitrogen cycle in freshwater ecosystems. Winter cover crops could contribute to more sustainable agricultural production and increase resiliency to climate change; however, their adoption remains low in California. This paper seeks to understand barriers to winter cover crop adoption by monetizing their long-term economic and agronomic impacts on farm profitability in two of California's specialty crop systems processing tomatoes and almonds. Our modeling effort provides a present, discounted valuation of the long-term use of winter cover crops through a cost-benefit analysis. A net present value model estimates the cumulative economic value of this practice. We then explore how the long-term trade-offs associated with winter cover crops can affect an operation's profits under a spectrum of hypothetical changes in California's agricultural landscape. Our analysis sheds light on the barriers to adoption by reporting benefit-cost ratios that indicate profitability across several scenarios; however, benefits and costs accrue differently over time and with long planning horizons. At the same time, a small portion of gained benefits are external to the grower. Findings from this study reveal that winter cover crops in California can be profitable in the long-term, but the extent of profit depends on the cropping system, extent of irrigation savings due to improved soil function, access to financial subsidies and climate change. Winter cover crops can return positive net benefits to growers who have flexible contractual obligations, can wait for the long-term return on investment and manage cover crops as closely as cash crops. This analysis contributes to the study of conservation agriculture practices by explaining possible reasons for low adoption through an economic valuation of the implications of soil management choices and policy counterfactuals. Sea-level rise is an inevitable consequence of climate change and threatens coastal ecosystems, particularly intertidal habitats that are constrained by landward development. Intertidal habitats support significant biodiversity, but also provide natural buffers from climate-threats such as increased storm events. Predicting the effects of climate scenarios on coastal ecosystems is important for understanding both the degree of habitat loss for associated ecological communities and the risk of the loss of coastal buffer zones. We take a novel approach by combining remote sensing with the IUCN Red List of Ecosystem criteria to assess this impact. We quantified the extent of horizontal intertidal rocky shores along ~200 km of coastline in Eastern Australia using GIS and remote-sensing (LiDAR) and used this information to predict changes in extent under four different climate change driven sea-level rise scenarios. We then applied the IUCN Red List of Ecosystems Criterion C2 (habitat degradation over the next 50 years based on change in an abiotic variable) to estimate the status of this ecosystem using the Hawkesbury Shelf Marine Bioregion as a test coastline.

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