Sweeneyosborn5043

The identified odorants were predominately monoterpenes and sesquiterpenes as well as organic acids. Several compounds including germacrene D, thymol, carvacrol, rotundone, β-caryophyllene, α-humulene, cinnamaldehyde, and ethyl cinnamate are reported here for the first time as odorous constituents of pinewood. Principal component analysis of the data obtained from sensory evaluation and cOEDA indicated that terpenes and sesquiterpenes were correlated with higher hedonic rates for the wood samples. Moreover, a Mantel test between the geographical distances of the trees and their sensory and cOEDA values demonstrated that the origin of the trees had a significant impact on the sensory characteristics of the wood, in agreement with the differences in the relative proportions of different volatile components. As such, the odor of Cembran pinewood is prone to variation but, yet, unique.Bimodal photoluminescence-magnetic resonance (MR) imaging technique has attracted tremendous attention due to its great potential in biomedical researches and clinical practices. Herein a novel multifunctional magnetoluminescent nanocomposite, FA-Gd-Tb@SiO2, was found to serve as an effective probe for bimodal time-gated luminescence/MR imaging of cancer cells in vitro and in vivo. The nanoprobe was designed by integrating a luminescent Tb3+ complex, a Gd(III)-based contrast agent and a tumor-targeting molecule, folic acid (FA), into a silica nanoparticle. The integration of these functional moieties allows the nanoprobe to be employed for specific imaging of cancer cells with background-free TGL and non-invasive MR imaging modes. In addition, the optical and magnetic properties were dramatically improved after implicating the newly synthesized nanoarchitecture. In vitro cellular TGL imaging demonstrated that the FA-Gd-Tb@SiO2 nanoprobe could recognize and accumulate in cancer cells overexpressing FA receptor. Furthermore, in vivo study revealed that the as-prepared nanoprobe was able to effectively enhance T1-weighted MR contrast and TGL intensity in tumor tissue, which might contribute to the precise detection and tracing of cancer cells, as well as diagnosis and therapy of tumor in clinical.We reported Eu(III) functionalized carbon dots (CDs-Eu) as a novel ratiometric fluorescent probe for point-of-care testing (POCT) of dipicolinic acid (DPA), a biomarker of anthrax. CM272 order The probe was synthesized simply by coordination interaction between Eu(III) ions and the CDs with carboxyl and amino groups. Upon addition of DPA, the intrinsic red luminescence of Eu(III) ions can be sensitized due to the presence of energy transfer when DPA chromophore coordinated with Eu(III) ions. The fluorescence peaking at 530 nm as a reference remained essentially constant, leading to ratiometric fluorescence response toward DPA. This approach exhibited a linearity range from 0.5 nM to 5 μM and a detection limit of 0.8 nM. More Importantly, the combination of probe-based test paper and smartphone provided a convenient approach for visual POCT of DPA with a detection limit of 67 nM, which was lower than other reported test paper for DPA detection. The color of fluorescence gradually changed from green to red as the DPA increases, which can be captured by a smartphone and further quantitatively analyzed with a built-in Color Analyzer App. The satisfactory result of detecting DPA accurately and reliably in real samples by the test paper certified its practicality in POCT of DPA.Nano-sized electrodes have their special advantages for sensing applications, such as small overall dimension, fast response and low background current. In this work, single gold nanoclusters (AuNCs) were controllably prepared on single Pt nanoelectrode surface by electrodeposition method. The AuNCs covered Pt nanoelectrode (AuNCs/PtNE) had steady-state voltammetric response in redox species solution, which was similar to micro-/nano-sized electrodes. It was interesting to find isonicotinic acid hydrazide (INH, also known as isoniazid) showed good electrochemical response on AuNCs/PtNE surface, which had investigated carefully by square wave voltammetry (SWV) and chronoamperometry. Moreover, the prepared single AuNCs/PtNEs showed the capability for INH sensing with good sensitivity, reproducibility and selectivity, which was demonstrated for INH detection in human urine samples.Fungi stand out as primary pathogens present in healthcare-acquired infections, presenting an increased number of cases even using appropriate antifungal therapy. Candida spp. is a predominant microorganism among several fungal pathogens present in the healthcare setting. Candidemia and candidiasis are fungal infections responsible for high morbidity and mortality among ill patients in hospitals. It is noticeable that prolonged hospital stays lead to a higher economic impact and increased risk for developing secondary fungal or even bacterial infections. New fast and sensitive approaches for the detection of Candida species is highly required. Electrochemical biosensors are an excellent alternative to conventional techniques by combining fast analyte detection, low cost, and the possibility of miniaturization. Lectins are carbohydrate-binding proteins with the capability to reach out to the microorganism cell wall. In this work, we proposed the development of an impedimetric biosensor for Candida spp. based on Concanavalin A (ConA) and wheat germ agglutinin (WGA) as recognition agents of the yeast cells. Atomic force microscopy images indicate changes in the biosensor surface after assembly of the molecules and exposure to fungal samples. Electrochemical impedance spectroscopy results revealed a proportional increase of charge transfer resistance (RCT) as fungal CFU increased, where four Candida species were evaluated (Candida krusei, Candida tropicalis, Candida parapsilosis and Candida albicans). The biosensor is useful to differentiate Candida spp. with a detection limit between 102 to 106 CFU mL-1. The obtained biosensor appears as an innovative candidate for the detection and differentiation of pathogenic Candida spp.In this paper, a high-performance enzyme cascade bio-platform has been developed for biosensing by combining MOFs-based nanozyme and natural enzymes. Firstly, a novel porous mixed bi-metal oxide (MnCo2O4) derived from MOF with rod-like nanostructures was synthesized. Based on this, the nanozyme of bovine serum albumin-Pt nanoparticles@mesoporous MnCo2O4 (BSA-PtNP@MnCo2O4) was successfully synthesized and used to construct enzyme cascade bio-platform. The nanozyme had unique physicochemical surface properties and hierarchical structure. Due to the synergistic effect of protein, bimetal oxide and PtNP, the nanozyme presented excellent dual enzyme activity. On the one hand, BSA-PtNP@MnCo2O4 can be used as nanozyme with oxidase activity to achieve superior detection of glutathione with detection limit of 0.42 μM. On the other hand, BSA- PtNP@MnCo2O4 can also be used both as the nanozyme with great peroxidase activity and as a scaffold for immobilization of glucose oxidase (GOx), guiding an organized high-efficiency enzyme cascade bio-platform. The platform combined advantages of nanozyme and natural enzyme, and provided excellent glucose detection with the detection limit of 8.1 μM. The tandem catalytic system not only broadened the application of nanozyme in natural enzyme catalysis, but also provided a simple, efficient and organized enzyme cascade bio-platform for biosensing and other applications.For the sake of effective colorimetric sensing-pattern, a sensitive colorimetric sensor was conceived based on polyoxometalates based metal-organic frameworks (POMOFs) and polydiallyldimethylammonium chloride functionalized reduced graphene oxide (PDDA-rGO) for the first time, in which PDDA as a "glue" molecule turns rGO nanosheets into general platforms for bonding POMOFs nanoparticles. Herein, a new POMOF compound with fascinating helices-on-helices feature, [Ni4(Trz)6(H2O)2][SiW12O40].4H2O (Trz = 1,2,4-triazole) (abbreviated as Ni4SiW12), was synthesized and characterized, then PDDA-rGO sheet as dispersive and conductive material was successfully introduced to Ni4SiW12 fabricating new PDDA-rGO/Ni4SiW12-n nanocomposites, (abbreviated as PMPG-n). The resulting PMPG-n nanocomposites as peroxidase mimetic show excellent catalytic activities under extreme condition (pH value 2.5), attributed to the nature and synergies from POMs, MOFs and PDDA-rGOs. Note that the peroxidase-like activity of PMPG-1 (the mass ratio of Ni4SiW12 to PDDA-rGO is 11) exhibits higher sensitivity (1-60 μM), faster response (10 min) and the lowest limit of detection (2.07 μM) among all reported materials to citric acid (CA) to date. This work opens up new application prospects in colorimetric sensing system for food quality control and safety, biotechnology and clinical diagnosis.Appropriate prescription of antibiotics requires the pharmacokinetic knowledge of the drugs and their metabolites in blood, and their distribution/retention in organ tissues. Here we report that highly crystalline graphite dots (GDs) allow for quantitative profiling of antibiotic metabolites in a spatial-temporal manner, in combination with matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI). GDs matrix features an ultra-clean background base line and high efficiency in ionization of small molecules, thus enabling quantification of sulfamethoxazole (SMZ) and its metabolites with limit of detection (LOD) in the femtomole range. Distinctly different from the other MS techniques, our approach is tolerant to high levels of salt contaminants in the complexed biological samples, thus minimizing the sample purification requirements and allowing for tests in small volumes. We have demonstrated quantitative measurements of the dynamic concentration changes of SMZ (m/z 276.27) and two metabolites, N4-hydroxy-SMZ (m/z 292.28) and N4-acetyl-SMZ (m/z 318.31) with only 1 μL mouse blood sample for each test. High-resolution distribution patterns of SMZ metabolites have directly been visualized a on the liver subsegments. Therefore, it allows for simultaneously acquisition of pharmacokinetic data in the blood combined with detailed hepatic zonation of SMZ metabolites for the first time. As a rapid, high-throughput platform to monitor small molecules in vivo, our approach of GDs-assisted MADLI MSI will foster the medical research on the antibiotic usage and drug development.A new method for fishing antitumor ingredients by G-quadruplex recognition from Macleaya cordata seeds extracts was established using a three-phase-laminar-flow-chip (TPL chip). The TPL chip integrated the separation of drugs from the complex ingredients and organic solvent extraction, simplifying pretreatment processes and reducing reagents and time. In addition, the chip method showed a lower false negative result, owing to the gentle membrane-free filtration process based on diffusion separation in microchannel. Four ligands with high content in alkaloids of Macleaya cordata seeds were selected, those are chelerythrine (CHE), sanguinarine (SAN), protopine (PRO), and allocryptopine (ALL), which demonstrated affinity with G-quadruplex and were potential for antitumor.