Lauesentermansen8777

Intracellular carbon monoxide (CO) is a gaseous signaling molecule and is generated enzymatically by heme oxygenases upon degradation of heme to billiverdin. Target structures for intracellular produced CO are heme proteins including cytochrome c oxidase of the respiratory chain, cytochrome P450-dependent monooxygenases, or myoglobin. For studies on CO signaling, CO-releasing molecules (CORMs) of different structure are available. Here, three frequently used CORMs (CORM-2, CORM-3 and CORM-401) were studied for their properties to provide CO in biological test systems and address susceptible heme proteins. CO release was investigated in the myoglobin binding assay and found to be rapid (50 min). Storage stability of CORM stock solutions was also assessed with the myoglobin assay. Only CORM-401 stock solutions were stable over a period of 7 days. Incubation of CORMs with recombinant cytochrome P450 led to an inhibition of enzyme activity. However, only CORM-3 and CORM-401 proved to be suitable in this test syst were less suitable. Depending on the experimental setting, data achieved with these compounds should be evaluated with caution. A neutral tea polysaccharide (TPSN) was isolated from green tea. Gas chromatography analysis showed that TPSN was composed of d-glucose, l-arabinose and d-galactose residues at a molar ratio of 90.0 9.1 0.9. The weight-averaged molecular weight of TPSN was determined as about 2.0 × 105 g mol-1 using static light scattering analysis. The result of nuclear magnetic resonance (NMR) spectroscopy indicated that TPSN and water-soluble starch had similar structures. TPSN exhibited inhibitory activity towards α-amylase through the noncompetitive inhibition mechanism, but the tertiary structure of α-amylase related to enzymatic activity, analyzed using circular dichroism spectroscopy, was not affected by TPSN. 17β-estradiol Meanwhile, TPSN exhibited hydrolysis properties catalyzed by α-amylase. Molecular docking analysis revealed that the various behaviors of TPSN to α-amylase could be attributed to that the different chain segments of TPSN combined with different amino acid residues of α-amylase. BACKGROUND Adolescents are among the highest consumers of sugar-sweetened beverages (SSBs) in the United States. More research is needed to understand the relationship of multiple levels of influence on adolescent SSB intake across the socioecological model in a nationally representative sample. OBJECTIVE This secondary analysis of cross-sectional data aims to explain variance in adolescent SSB intake by exploring the associations of adolescent demographic (ie, age, race/ethnicity, and parent socioeconomic status), intrapersonal (ie, behavioral intention, self-efficacy, and media perception), interpersonal (ie, social norms and perceived parenting practices), and home availability variables. DESIGN This study included 1,560 adolescents who participated in the 2014 National Cancer Institute-sponsored cross-sectional Family, Life, Activity, Sun, Health, and Eating study. Descriptive statistics, analyses of variance, and stepwise multiple linear regression models were used to explore factors associated with SSB ence on adolescent's health behaviors may also be a key intervention target. Home and parental SSB factors may be more important than targeting intrapersonal factors and social norms among adolescents. BACKGROUND Plasma triglyceride (TG) concentrations are markedly higher among Asians, which may be associated with the interaction of genetics and lifestyle factors. OBJECTIVE The purpose of this study was to investigate the genetic variants that have a strong association with plasma TG concentrations from genome-wide association study and to identify lifestyle interactions with the genetic variants that are associated with dyslipidemia in a cohort of Korean adults. DESIGN Korean genome and epidemiology study utilized a cross-sectional design of Koreans to determine genetic variants and lifestyle factors, including nutrient intakes, in a retrospective hospital-based city cohort conducted by the Korean Center for Disease and Control during 2004-2013. PARTICIPANTS Korean adults aged 40 to 77 years were participants (n=28,445). MAIN OUTCOME MEASURES The genetic variants that influence plasma TG concentrations were selected by genome-wide association study using an allele genetic model after adjusting for age, sexajor alleles. Carriers of the minor alleles with low calcium intakes ( less then 500 mg/day) experienced elevated plasma TG concentrations compared with carriers of the major alleles. Smokers and alcohol drinkers with either of the minor alleles of APOA5, rs662799 or rs2266788, had higher plasma TG concentrations than those with its major allele. CONCLUSIONS These results indicated that carrying the minor alleles of APOA5 rs662799 and rs2266788, especially for men, was associated with elevated TG concentrations and suggested that Korean carriers of the minor alleles could be at increased risk of hypertriglyceridemia. Further research is needed to investigate the efficacy of modulating lifestyle factors to prevent dyslipidemia in people carrying the minor alleles of APOA5 rs662799 and rs2266788. BACKGROUND Lowering excess sodium in packaged foods is part of a public health strategy to reduce cardiovascular disease risk. Sales of foods with labeled sodium claims increased during the past decade. Yet, it is unclear whether sugars or fats were added during the reformulation of foods that might counter the benefits of sodium reduction. OBJECTIVE It was hypothesized that the nutrient content of packaged foods with lower sodium label claims (ie, sodium-modified) would differ from their regular (ie, unmodified) counterparts. DESIGN This cross-sectional study compared label data of 153 sodium-modified foods and 141 regular, matched counterparts within four food categories soups, processed meats, vegetables, and savory snacks. Foods were identified by searching manufacturer websites of the top-10 brands in each category. Sodium, calories, total carbohydrate, sugar, protein, total fat, saturated fat, and potassium (when reported) were compared by labeled serving and per 100 g food. RESULTS The average amount in milligrams of sodium per serving in regular foods ranged from 162 mg for savory snacks to 782 mg for soups. Compared with regular foods, the matched lower sodium foods had significantly less sodium per serving (-95 to -387 mg) and per 100 g (-184 to -462 mg) (P0.05 for all comparisons). Of the soups that reported potassium on the label, potassium was 244 mg/serving (P=0.004) and 139 mg/100 g (P=0.002) higher among matched lower sodium soups. CONCLUSIONS The similarity in macronutrient contents on the labels for sodium-modified foods and their regular counterparts suggests that reformulation did not include the addition of significant amounts of sugars, fats, or other macronutrients among major food brands in the selected categories. Potassium content and additional food categories deserve further investigation. BACKGROUND There is a dearth of evidence on geographic variation in sleep duration and quality, and about the effect of geographic location or "place" on sleep. The objective was to assess the magnitude of geographic variation in sleep duration and sleep quality in Canada, while controlling for individual-level factors. METHODS Data from the 2015-2018 cycles of the Canadian Community Health Survey were used. The sample consisted of 96,484 respondents from 6 provinces. link2 Multilevel logistic regression techniques were used to assess the magnitude of geographic variation in self-reported measures of short and long sleep duration and 3 indicators of sleep quality (difficulty initiating and maintaining sleep, daytime sleepiness, and finding sleep refreshing) across geographic areas, defined by the boundaries of Forward Sortation Areas. RESULTS Overall, 45.31% of respondents reported short sleep, 2.31% reported long sleep, 46.97% had difficulty initiating and maintaining sleep, 29.50% had daytime sleepiness, and 39.11% did not find their sleep refreshing. After controlling for individual-level factors, geographic variation accounted for 4.00% and 13.67% of overall variance in short and long sleep duration, respectively; the corresponding estimates for difficulty initiating and maintaining sleep, daytime sleepiness, and finding sleep refreshing were 3.04%, 3.80%, and 5,08%, respectively. CONCLUSIONS There is a significant level of geographic variation in short and long sleep duration and sleep quality and this variation cannot be accounted for by differential distribution of individual characteristics across geographic areas. Future research is warranted to examine specific contextual factors that can account for this variation. OBJECTIVES We sought to examine the association of positive and negative experiences using social media (SM) and sleep disturbance in a national survey of U.S. young adults. METHODS Experiences using SM were assessed with 2 items asking participants about the percentage of time using SM that involved a negative/positive experience that they were personally involved in. Sleep disturbance was assessed using the validated PROMIS 4-item short form. Ordinal logistic regression was used to examine associations between positive and negative SM experiences and high sleep disturbance, while controlling for relevant covariates. RESULTS Although reporting high levels of negative experiences was significantly associated with greater odds of high sleep disturbance (AOR = 1.49, 95%CI = 1.18-1.89), reporting high levels of positive experiences was not associated with sleep disturbance. CONCLUSIONS These findings suggest that more robust examinations of negative SM experiences-especially as they relate to sleep disturbance-may be warranted. BACKGROUND The objective of this systematic review and meta-analysis was to investigate the associations between neighborhood socioeconomic status (nSES) and sleep duration in children aged 0-18 years. METHODS Three electronic databases were searched for relevant articles that assessed nSES and sleep duration (either subjectively or objectively). Inclusion criteria included peer-reviewed scholarly articles on the topic area that reported an association between nSES and sleep in children and adolescents. link3 RESULTS The database searched identified 6080 potentially eligible studies, of which 1210 were selected for full-text review, and 8 met the inclusion criteria. Data included 67,677 unique participants. Studies were conducted in either the United States of America or Australia. Pooled estimates suggested that poorer nSES was associated with shorter child sleep duration (odds ratio 1.262; 95% confidence interval 1.086-1.467). This relationship between nSES and sleep was moderated by sleep assessment type (self-report versus actigraphy), child sex/gender, and child race/ethnicity. CONCLUSIONS Across studies, there is an association between nSES and child sleep duration. This study adds child sleep to the growing number of child health disparities associated with nSES. OBJECTIVES Sleep is essential for health and well-being and lack of sleep can have serious physiological consequences. This study aimed to evaluate sleep patterns and the influence of insomnia on quality of life. DESIGN The epidemiologic sleep study is a population-based study of sleep and risk factors for sleep disturbances. SETTING AND PARTICIPANTS This cross-sectional study recruited 574 men and 468 women aged 18 years or older randomly, to represent the population of Sao Paulo, according to gender, age, and socioeconomic status. MEASUREMENTS Data from polysomnography were used to assess sleep objectively and a validate questionnaire to assess quality of life. Validated questionnaires based on the Diagnostic and Statistical Manual of Mental Disorders, fourth edition, assessed the perception of insomnia and categorized the sample as without insomnia symptoms, insomnia symptoms, and insomnia syndrome. Anthropometric data, objective sleep parameters and quality of life were assessed and the sample was distributed according to age for both genders.