Emeryemborg4125

Malignant melanoma is a skin cancer with a high rate of metastasis. Numerous circular RNAs (circRNAs) have been shown to play vital roles in melanoma. This research aimed to investigate the role and molecular basis of circ_0016418 in melanoma progression.

The abundanced of circ_0016418, miR-605-5p and glutaminase (GLS) were measured using quantitative real-time polymerase chain reaction or western blot analysis. Cell proliferation was evaluated using Cell Counting Kit-8 (CCK-8) assay and colony formation assay. Cell migration and invasion were assessed by transwell assay. Cell cycle and apoptosis were monitored by flow cytometry. The levels of glutamine consumption and glutamate were examined using commercial kits. The interaction among circ_0016418, miR-605-5p and GLS was verified with the dual-luciferase reporter assay. A xenograft model was used to analyze tumor growth

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Circ_0016418 and GLS were up-regulated, while miR-605-5p was down-regulated in melanoma tissues and cells. Circ_0016418 silencing hindered cell proliferation, metastasis, and glutamine catabolism and promoted cell cycle arrest and apoptosis in A375 and A875 cells. Circ_0016418 modulated melanoma progression and glutamine catabolism through sponging miR-605-5p. Also, miR-605-5p inhibited melanoma progression and glutamine catabolism by targeting GLS. Moreover, circ_0016418 depletion blocked tumor growth

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Knockdown of circ_0016418 suppressed melanoma development and glutamine catabolism by modulating the miR-605-5p/GLS pathway.

Knockdown of circ_0016418 suppressed melanoma development and glutamine catabolism by modulating the miR-605-5p/GLS pathway.Mesenchymal chondrosarcoma of the kidney is a rare soft tissue sarcoma. We report a 64-year-old female with a right renal pelvic mass. After a right nephrectomy was performed, the histopathological examination confirmed the diagnosis to be primary extraskeletal mesenchymal chondrosarcoma. After the surgical removal of the tumor, the patient suffered rapid disease progression.Extra-adrenal paraganglioma is rare, and occurs in the retroperitoneum, and head and neck. The incidence rate of paraganglioma in urogenital system is very low, especially in the spermatic cord. A case of paraganglioma of spermatic cord is reported and relevant literature is reviewed. A spermatic cord mass was found in the right scrotum in a middle-aged man 2 years ago, without hormone symptoms. Understanding of clinical and intraoperative frozen pathology was inadequate. Ultrasound images showed that there was abundant blood supply around the right spermatic cord with clear boundaries. The conventional pathology of the tumor presented a typical histologic morphology of paraganglioma. Immunohistochemistry showed that chief tumor cells were CGA (+), syn (+), CD56 (+), SDHB (+), and sertoli cells were S-100 (strong+). There are few reported cases at present, and the etiology and pathogenesis are not clear yet. The rapid frozen pathologic diagnosis during operation is very challenging, and it is easily diagnosed by routine histology combined with immunohistochemistry. Gene detection is recommended if necessary. Early diagnosis is helpful to the choice of operation mode and the prevention and control of intraoperative risk.Myopericytoma is an uncommon, slow-growing benign tumour of concentrically distributed perivascular myoid cells, that occurs generally in the skin and superficial soft tissues especially in distal extremities. In the visceral organs, it is particularly rare. We provide the first report of this rare entity in the stomach. A 45-year-male presented to an outside hospital because of pharyngalgia and cough 10 days prior. Endoscopic ultrasonography revealed a 0.92 cm × 0.92 cm hypoechoic lesion in the submucosa of sinuses ventriculi. For further diagnosis and treatment, the patient came to our hospital, and underwent endoscopic submucosal excavation (ESE), without adjuvant therapy. Postoperative pathology was myopericytoma. No recurrence was found in the follow-up of 27 months. In conclusion, myopericytoma is a comparatively newly described disease entity approved by the World Health Organization classification for tumours of soft tissue. The present report shows the first case of myopericytoma of the stomachto remind clinicians and pathologists that myopericytoma may be encountered at this location.Multiple gastrointestinal stromal tumors (GISTs) are extremely rare, usually related to specific tumor syndromes such as familial GIST syndrome, neurofibromatosis type 1 (NF1), and the Carney triad. A 27-year-old man came to the hospital for treatment due to watery bloody stool. Abdominal CT disclosed multiple tumors in the gastrointestinal tract. The postoperative pathological examination showed multiple GISTs and diffuse hyperplasia of interstitial cells of Cajal. The c-KIT mutation at exon 11 c.1676T>C (p.V559A) was detected in the paraffin-embedded tumor tissue. He had skin hyperpigmentation from childhood, but had no family history of GIST. This case of multiple GISTs without family history attracted our attention.Lymphoblastic lymphoma (LBL) is a type of non Hodgkin's lymphoma. It is highly malignant and aggressive. Most patients have poor prognosis. learn more Extramedullary involvement of B-LBL is very common, and the most vulnerable tissues are skin, bone, and soft tissues. Primary renal B-LBL is rarely reported. In this article, we report an 8-year-old boy who was admitted to hospital due to abdominal pain and vomiting. He was diagnosed with B lymphoblastoma by CT guided renal biopsy and bone marrow puncture. We review the clinical characteristics and diagnosis and treatment process of this case.Lymphoproliferative disorders of natural killer cells (LPD-NK) are rare lymphoproliferative diseases involving NK cells. Here, we present two cases of LPD-NK. The first case is a 63-year-old man who presented with high fever, cytopenia, and a history of myelodsyplastic syndrome. He was finally diagnosed with aggressive NK cell leukemia and died due to progression of the disease within 15 days of diagnosis. The second case is a 70-year-old man with granulocytopenia who did not have clinical manifestations; he was diagnosed with chronic lymphoproliferative disorder of NK cells and a watch and wait approach was adopted until six-month follow up. This article describes the clinical features, pathogenesis, diagnosis, treatments, and prognosis of LPD-NK through a literature review of case reports.