Yildirimtherkelsen4836

Chitosan can be produced through the enzymatic process catalyzed by chitin deacetylase which can be produced by bacteria. The biotransformation of chitin to chitosan by bacteria is interesting because the process is economical and environmentally friendly. This study described the potential of sponge-associated bacterium capability in degrading chitin and forming chitosan.

The bacteria were isolated from sponge Cribrochalina sp. this website at Manado Bay, Indonesia. In the screening of the chitinase activity of bacteria, chitin media was used. Meanwhile, the transformation of chitin to chitosan was tested by using Chitinase Degrading Activity media. Molecular identification of bacteria was based on 16S rRNA gene sequences.

The results showed that the SS1, SS2, SS3, SS4 and SS5 bacterial isolates could degrade chitin based on chitinolytic indexes. These five bacteria could also form chitosan exhibited through the presence of chitosan in the form of precipitation in the fermented broth of bacteria. SS1 had the highest chitinase activity based on the chitinolytic index identified as Bacillus subtilis (100% identity), hence it is called B. subtilis strain SS1. The partial rRNA gene sequences data were deposited at GenBank under accession number MN999892.

The bacteria strain isolated from Cribrochalina sp. can be utilized in degrading chitin and form chitosan which could be a promising candidate for an economical and eco-friendly process of chitosan.

The bacteria strain isolated from Cribrochalina sp. can be utilized in degrading chitin and form chitosan which could be a promising candidate for an economical and eco-friendly process of chitosan.

Chillies are delicious spices that are used extensively. Capsaicinoids, the major constituents of chillies with reported anti-cancer effects, have been determined with non-specific colorimetric methods. A rapid and reproducible method for extraction and quantification of the major chillies capsaicinoids; capsaicin, dihydrocapsaicin (DHC) and nordihydrocapsaicin (n-DHC), was reported, moreover study of their cytotoxic activity.

This study has covered the extraction of capsaicinoids from red and green-colored chillies followed by their quantification using HPLC-UV method after validation. Furthermore, the correlation of capsaicinoids contents with their in vitro hepatocarcinoma cytotoxicity was represented by Pearson's correlation coefficient.

Capsaicinoids contents are ranged from 1219.88-15098.67 ng mg-1 of Dried Extract (DE). Capsaicin exhibits the lowest IC50 when compared to doxorubicin (9.201±0.91 and 16.1±0.82 μg mL-1, respectively). The exhibited activities of methanol extracts of red and green-colored chillies (IC50 = 20.21±1.72 and 16.02±0.69 μg mL-1, respectively) may attribute to their excessive contents of capsaicinoids (6975.42 and 15098.67 ng mg-1 DE, respectively). Capsaicin and n-DHC contents have a negative correlation with cytotoxic activity.

Green-colored chillies were found to be more cytotoxic in comparison with red-colored chillies that may be relative to their high content of capsaicinoids. The present investigation suggests that capsaicinoids contents correlate with cytotoxic activity.

Green-colored chillies were found to be more cytotoxic in comparison with red-colored chillies that may be relative to their high content of capsaicinoids. The present investigation suggests that capsaicinoids contents correlate with cytotoxic activity.

Prenatal exposure to ionizing radiation can interfere with embryonic and fetal growth depending on the dose and gestational age. The present study was completed to evaluate the effect of transplanted bone marrow on the fetal skeleton of pregnant rats exposed to gamma radiation.

Experimental animals were separated into 5 groups C group, R7 group, R7+BM group, R14 group and R14+BM group. All pregnant rats were sacrificed on day 20 days of gestation and the skeletal systems of the fetuses were examined and photographed. This study focused on skull, upper and lower jaw, occipital region, sacral and caudal region, fore and hind limbs.

Gamma rays caused any disturbance in the ossification process of the skull bones, upper and lower jaws, occipital bones, it caused the loss of some ossification centers in metacarpal bones, metatarsal bones but bone marrow transplantation greatly reduced the injury that happened because of γ-radiation.

This study showed that transplantation of bone marrow post-irradiation in pregnant rats could reduce the hazards of gamma-irradiation in the different regions of the fetal skeleton.

This study showed that transplantation of bone marrow post-irradiation in pregnant rats could reduce the hazards of gamma-irradiation in the different regions of the fetal skeleton.

Leek (Allium ampeloprasum) is one of the most commonly used herbal foods all over the world. This study was conducted to evaluate the protective effect of leek extract on catfish experimentally challenged with Aeromonas hydrophila, a problematic bacterial pathogen that affects various freshwater fish species.

Aeromonas hydrophila was isolated and identified from catfish showing clinical signs of septicemia. The in vitro activity of leek extract to control the growth of Aeromonas hydrophila was investigated. In the in vivo experiment, about 240 adult catfish (Clarias gariepinus) were fed three different leek extract concentrations (10, 25 and 50 mg kg-1 body weight) for 1 month. Later on, a challenge study was conducted using an identified A. hydrophila strain. Morbidity and mortality were recorded throughout one week post-challenge. Furthermore, the effect of leek extract on some immune-related genes was investigated.

Under the in vitro testing, a significant increase (10 and 13 mm) in the inhibition zone was recorded in wells treated with 25 and 50 mg L-1 leak extract, respectively. A significant reduction in fish mortalities was reported in all leek extract treated groups compared to the control group which was given water. TLR1 gene expression was upregulated in fish treated with leek extract while TNFα gene expression was down-regulated.

Overall, results suggested that the leek extract has immunostimulating effects that can help control bacterial infections in catfish and probably other fish species.

Overall, results suggested that the leek extract has immunostimulating effects that can help control bacterial infections in catfish and probably other fish species.