Tobindalgaard3125
To evaluate the effect of proliferating cell nuclear antigen (
) and
in patients with oral squamous cell carcinoma (OSCC).
Multiple databases, including PubMed, Embase, Cochrane library, and China National Knowledge Database, were searched for relevant studies and full-text articles that evaluated the effect of
and
in patients with OSCC. Review Manager 5.2 was adopted to estimate the impact of the results among the selected articles. Forest plots, NOS table, sensitivity analysis, and bias analysis were also conducted.
In total, nine eligible studies satisfied the included criteria. High
expression (>50%) was significantly more prevalent in OSCC than low
expression (<50%) (OR =3.88; 95% CI 2.04-7.37; P<0.0001; I
=0%). However, there was no significant difference between
and OSCC (OR =1.60; 95% CI 0.18-14.63; P=0.68; I
=86%). Low
expression had a higher 5-year overall survival in OSCC patients than high
expression (OR =0.47; 95% CI 0.27-0.80; P=0.005; I
=41%). Meanwhile,
negative had a higher 5-year overall survival than
positive (OR =0.20; 95% CI 0.10-0.42; P<0.0001; I
=0%). There was no difference between high and low
in terms of metastasis (OR =0.80 with 95% CI 0.18-3.45, I
=63%, P of over effect =0.76). The overall results showed no difference between
and metastasis (OR =0.38 with 95% CI 0.13-1.10, I
=0%, P of over effect =0.07).
and
might be suitable for prognostic and survival evaluation in OSCC patients.
PCNA and p53 might be suitable for prognostic and survival evaluation in OSCC patients.
Increased CD11c
Mφ aggravates colonic mucosal injuries in ulcerative colitis (UC) with TSP1 protein increased. The thrombospondin-1 (TSP1) protein which could activate Mφ is closely related to the colonic mucosal damage in UC. Here, we investigated the role of TSP1 in the differentiation of CD11c
Mφ and the mechanism.
We analyzed the population characteristics of
genes using the Genotype-Tissue Expression (GTEx) database, and human serum TSP1 protein was detected with ELISA. DSS-induced colitis rats were used to explore the effects of TSP1 on colonic mucosal inflammation. We analyzed the serum cytokines and tissue histopathology to evaluate the severity of UC. Furthermore, we analysed the main source of TSP1 in colon tissue. In vitro, lamina propria mononuclear cells (LPMC) and CD11c
lamina propria macrophages (LPMP) was isolated from model rats
. The target of TSP1 protein was assessed by LSKL,
and
interfering plasmids. The proteins, the lysosome, lysosomal activity and Cathepsin E activ pathway, which aggravates the colonic mucosal inflammatory injuries in UC.
TSP1 promotes the migration and the differentiation of CD11c+ LPMP with lysosomal activity limited via activating the CD36-PRKCQ/NF-κB signaling pathway, which aggravates the colonic mucosal inflammatory injuries in UC.
Mediastinal cysts (MCs) can be misdiagnosed as mediastinal tumors (MTs) such as thymomas on the basis of radiological examinations, including computerized tomography (CT) and magnetic resonance imaging (MRI). Our study aimed to determine the utility of a radiomics model combined with eXtreme Gradient Boosting (XGBoost) for diagnosing anterior mediastinal masses.
Patients with anterior mediastinal lesions admitted to Shanghai Pulmonary Hospital between October 2014 and January 2018 were enrolled in the study. Mediastinal lesions were sketched on each CT image frame using OsiriX workstation. The study involved a total of 592 patients (289 male/303 female; age range, 18-83 years) with anterior mediastinal lesions (322 MCs and 270 MTs). Previously collected training data was used to build an XGBoost model to classify MCs and MTs, and a prospectively collected training dataset and external data from Huashan Hospital were used for validation. The SHapley Additive exPlanations (SHAP) method was used to help understand the complex model.
The XGBoost model was established using 107 selected radiomic features, and an accuracy of 0.972 [95% confidence interval (CI) 0.948-0.995] was achieved compared to 0.820 for radiologists. For lesions smaller than 2 cm, XGBoost model accuracy reduced slightly to 0.835, while the accuracy of radiologists was only 0.667. The model accuracy also achieved 0.910 when validated using an independent external dataset containing 87 cases. SHAP analysis suggested the 90% percentile Hounsfield unit value as a promising diagnostic parameter.
Our combined radiomics and XGBoost model significantly increased the accuracy of distinguishing between MCs and MTs compared to the level of accuracy obtained by radiologists.
Our combined radiomics and XGBoost model significantly increased the accuracy of distinguishing between MCs and MTs compared to the level of accuracy obtained by radiologists.
Bacterial contamination still poses serious challenges to blood safety. Platelets have the highest bacterial contamination risk of all blood components.
Twenty units of manual platelets were prepared from blood donated by our hospital, which were inoculated with
and
suspensions. The riboflavin sodium phosphate solution was added into platelets, adjusted to a final concentration of 160 μmol/L. Platelets added into an illumination bag and placed in the inactivation system for riboflavin photochemistry at various doses. The inactivation effect of bacteria was evaluated on a Columbia blood agar plate by the plate counting method. Meanwhile, the blood routine, blood gas analysis, platelet aggregation test, and thromboelastogram of platelets before and after treatment were detected to evaluate the changes of platelet quality after treatment.
the inactivation effect of
and
at the inactivation dose (16.9 J/cm
) could reach more than 4 logs. PF07220060 After treatment at 16.9 J/cm
, the blood routine results ng little impact on platelet function.
Delayed aneurysm rupture after flow diverters (FDs) is a serious complication which mechanism remains unclear. The hemodynamics of FDs with proximal or distal densification implantation strategies have rarely been reported. In this study, we investigated not only the hemodynamic factors involved in postoperative rupture, but also the hemodynamic effects of different FDs implantation strategies on avoiding this complication.
We selected 2 internal carotid artery (ICA) aneurysms with similar morphological characteristics, both of which were treated with FDs but had opposite therapeutic outcomes (Case 1, ruptured after FD treatment; Case 2, recovered). The FDs strategies we designed were strategy A [with homogeneous 30% metal coverage ratio (MCR)], strategy B (with distal densification of 40% and proximal 30% MCR) and strategy C (with proximal densification of 40% and distal 30% MCR). Virtually FDs deployment and computational fluid dynamics (CFD) method were performed to simulate FDs implantation strategiesms. In addition, the hemodynamic effects were favorable when the FD was improved to proximal densification, which may reduce the risk of delayed aneurysm rupture following FDs treatment.
Delayed rupture; flow diverter (FD); computational fluid dynamics (CFD); intracranial aneurysm (IAs); internal carotid artery (ICA).
Delayed rupture; flow diverter (FD); computational fluid dynamics (CFD); intracranial aneurysm (IAs); internal carotid artery (ICA).
The anti-tumor effect of interleukin (IL)-36β-mediated activation of CD8
T cells has been reported, but the molecular mechanism is largely undefined.
The levels of IL-36β in pancreatic cancer were examined by quantitative real-time PCR (qRT-PCR) and immunohistochemical staining. Cytology and animal experiments were performed to study the effects of IL-36β on the growth of pancreatic cancer cells. We then examined the changes of CD8
T cells and natural killer (NK) cells in the tumor by flow cytometry. The microRNA expression profiles were determined by microarray analysis.
The results revealed decreased levels of IL-36β in pancreatic cancer tissues. In addition, IL-36β inhibited tumor growth and promoted CD8
T and NK cell proliferation in the tumor microenvironment (TME). Moreover, IL-36β stimulated CD8
T cells to synthesize high amounts of interferon-gamma (IFN-γ) and IL-2. Microarray analysis showed that IL-36β administration to human and mouse CD8
T cells consistently downregulated the miRNA, let-7c-5p. Downregulation of let-7c-5p resulted in IFN-γ and IL-2 upregulation in CD8
T cells, whereas its upregulation had the opposite effect. Further experiments demonstrated that IL-36β downregulated IFN-γ in let-7c-5p
CD8
T cells.
These findings suggest IL-36β promotes IFN-γ and IL-2 production in CD8
T cells, as well as anti-tumor effects in CD8
T cells by downregulating let-7c-5p.
These findings suggest IL-36β promotes IFN-γ and IL-2 production in CD8+ T cells, as well as anti-tumor effects in CD8+ T cells by downregulating let-7c-5p.
Laparoscopic hepatectomy (LH) for hepatocellular carcinoma (HCC) remains controversial due to limited research. This study analyzed the oncology prognosis of patients who received LH treatment for HCC compared with conventional open hepatectomy (OH).
We conducted a retrospective analysis of patients with cirrhosis who underwent hepatectomy for HCC between 2012 and 2018. Patients were divided into LH and OH groups, and the oncology outcomes were compared before and after 11 propensity score matching (PSM).
A total of 403 patients with HCC cirrhosis who received LH (n=112) and OH (n=291) were enrolled. After PSM, 106 pairs of patients were matched. Compared with OH before and after PSM, there was no significant difference in overall survival (OS) and relapse-free survival (RFS) between the two groups. Tumor stage, Child-Pugh classification, venous tumor thrombus, tumor size ≥5 cm, and microvascular invasion (MVI) were independent risk factors for postoperative OS in HCC patients with cirrhosis. Tumor size ≥5 cm and MVI were independent risk factors for RFS.
Patients with HCC who underwent LH had a similar OS and RFS compared with those who received traditional open surgery. Therefore, LH can be used as a safe and feasible treatment for patients with HCC.
Patients with HCC who underwent LH had a similar OS and RFS compared with those who received traditional open surgery. Therefore, LH can be used as a safe and feasible treatment for patients with HCC.
Evaluated plasma homocysteine (Hcy) is an independent risk factor for cardiac fibrosis which is a common feature of cardiovascular disease, although the mechanisms are still unclear. This study aims to explore the mechanism of Hcy-induced cardiac fibrosis.
The mRNA and protein levels of Forkhead box O3 (FoxO3) and differentiation markers were detected in primary cardiac fibroblasts (CFs) after 300 µM Hcy treatment. Scratch and transwell migration assay were used to determine the effect of Hcy on proliferation and migration in CFs. The protein levels involved in the fibrotic processes in mice fed with high methionine diet (HMD) for 4 or 8 weeks were investigated by western blot. CFs were infected with FoxO3 recombinant adenovirus to explore the potential role of FoxO3 in Hcy-induced cardiac dysfunction.
Hcy treatment significantly promoted the differentiation, proliferation and migration of CFs, while FoxO3 activity were decreased in CFs. In HMD hearts, the protein levels of TIMP1, Fibronectin and α-SMA were increased after 4 or 8 weeks, but the FoxO3 activity was decreased.
