Stampemathiesen0654

Hepatozoon spp. are blood protozoans that can infect snakes. Infections with these parasites are found in more than 200 snake species and are considered to be the most frequent hemogregarines in snakes. In this study, a total of 73 dead snakes in captivity conditions, belonging to six different species, which were collected between June 2018 to October 2020 from different parts of Iran, were examined for the presence of blood parasites with microscopy and molecular methods. DNA was extracted from the removed heart, and PCR was done with two pairs of primers targeting the 18S rRNA gene. By microscopy, Hepatozoon spp. were detected in the heart blood of 29 out of the 73 (39.72%) snakes. From these 29 positive samples, eight were found to be positive using the PCR method. This study aimed to report for the first time the molecular characteristics of Hepatozoon spp. infecting venomous terrestrial snakes in Iran.

There is growing recognition that COVID-19 does cause cardiac sequelae. The underlying mechanisms involved are still poorly understood to date. selleck products Viral infections, including COVID-19, have been hypothesized to contribute to autoimmunity, by exposing previously hidden cryptic epitopes on damaged cells to an activated immune system. Given the high incidence of cardiac involvement seen in COVID-19, our aim was to determine the frequency of anti-DSG2 antibodies in a population of post COVID-19 patients.

300 convalescent serum samples were obtained from a group of post COVID-19 infected patients from October 2020 to February 2021. 154 samples were drawn 6months post-COVID-19 infection and 146 samples were drawn 9months post COVID infection. 17 samples were obtained from the same patient at the 6- and 9- month mark. An electrochemiluminescent-based immunoassay utilizing the extracellular domain of DSG2 for antibody capture was used. The mean signal intensity of anti-DSG2 antibodies in the post COVID-19 samples was significantly higher than that of a healthy control population (19±83.2 in the post-COVID-19 sample vs. 2.1±7.2 (p<0. 0001) in the negative control healthy population). Of note, 29.3% of the post COVID-19 infection samples demonstrated a signal higher than the 90th percentile of the control population and 8.7% were higher than the median found in ARVC patients. The signal intensity between the 6-month and 9-month samples did not differ significantly.

We report for the first time that recovered COVID-19 patients demonstrate significantly higher and sustained levels of anti-DSG2 autoantibodies as compared to a healthy control population, comparable to that of a diagnosed ARVC group.

We report for the first time that recovered COVID-19 patients demonstrate significantly higher and sustained levels of anti-DSG2 autoantibodies as compared to a healthy control population, comparable to that of a diagnosed ARVC group.Metal-organic frameworks (MOFs) represent a class of nanoporous materials built up by metal ions and organic linkers with several interesting potential applications. The present study described the synthesis and characterization of Gd(III)-based MOF with the chemical composition [Gd(BTC)(H2O)]·DMF (BTC - trimesate, DMF = N,N'-dimethylformamide), known as MOF-76(Gd) for hydrogen adsorption/desorption capacity and humidity sensing applications. The structure and morphology of as-synthesized material were studied using powder X-ray diffraction, scanning and transmission electron microscopy. The crystal structure of MOF-76(Gd) consists of gadolinium (III) and benzene-1,3,5-tricarboxylate ions, one coordinated aqua ligand and one crystallization DMF molecule. The polymeric framework of MOF-76(Gd) contains 1D sinusoidally shaped channels with sizes of 6.7 × 6.7 Å propagating along c crystallographic axis. The thermogravimetric analysis, heating infrared spectroscopy and in-situ heating powder X-ray diffraction experiments of the prepared framework exhibited thermal stability up to 550 °C. Nitrogen adsorption/desorption measurement at -196 °C showed a BET surface area of 605 m2 g-1 and pore volume of 0.24 cm3 g-1. The maximal hydrogen storage capacity of MOF-76(Gd) was 1.66 wt % and 1.34 wt % -196 °C and -186 °C and pressure up to 1 bar, respectively. Finally, the humidity sensing measurements (water adsorption experiments) were performed, and the results indicate that MOF-76(Gd) is a suitable material for moisture sensing application with a fast response (11 s) and recovery time (2 s) in the relative humidity range of 11-98%.Tris (1,3-dichloro-2-propyl) phosphate (TDCPP), a chlorinated organophosphate flame retardants(OPFRs), is widely used in a range of plastic foams, resins, and latexes. It can be detected in human tissues, including urine, and milk. Recent research has suggested that TDCPP has neurotoxic, reproductive, and potentially carcinogenic. In our study, we proposed a novel method for predicting the gene associated with tumor-compound interactions. We firstly used The Comparative Toxicogenomics Database (CTD) and downloaded potentially interactive genes about TDCPP in renal carcinoma. Gene expression data and the corresponding clinical information of the Kidney renal clear cell cancer (KIRC) patients were obtained from The Cancer Genome Atlas database (TCGA). Data from normal people in The Genotype-Tissue Expression (GTEx) databases was used to supplement the calculations. After being predicted by PharmMapper database, and validated by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes, 25 genes were selected to prevention of diseases.The effects of C/N ratio in mainstream partial nitritation (PN)-anaerobic ammonia oxidation (ANAMMOX) considering competitive relationship of aerobic microbes competing for oxygen were investigated. Thy system was operated for 501 d with various C/N ratio. Competitive growth of aerobic heterotrophic bacteria (AHB) at ≥ 1 of C/N ratio acted effectively on the selective inhibition of nitrite-oxidizing bacteria (NOB) while contributing to stable PN-A. In-depth kinetic analysis indicated oxygen affinity of aerobic microbes was in the order of AHB > ammonia-oxidizing bacteria (AOB) > NOB. In addition, potential of denitritation by AHB could contributed to improving nitrogen removal up to 87.5 ± 4.3%. AHB was comparatively clustered into two groups with a C/N ratio of 1. Nitrosomonas sp. PY1 became predominant while Nitrospira spp. were the major NOB. The potential of AHB in establishing selective inhibition of NOB was identified, which could be a novel approach to stabilze the mainstream PN-A.Microorganisms affect cadmium (Cd) extraction by hyperaccumulators to varying degrees, but the potential mechanism has not been completely studied. Here, two plant growth-promoting bacteria (PGPB, Bacillus paranthracis NT1 and Bacillus megaterium NCT-2) were assessed for their influence on Cd uptake by Solanum nigrum L. and their influence mechanisms. The results showed that both two strains could regulate phytohormones secretion, alleviate oxidative stress and promote S. nigrum growth when exposed to Cd (dry weight was significantly increased by 21.51% (strain NCT-2) and 21.23% (strain NT1) compared with the control, respectively). Additionally, strain NCT-2 significantly elevated the translocation factor (TF) and bioconcentration factor (BCF), and thus significantly facilitated total Cd uptake by 41.80% of S. nigrum, whereas strain NT1 significantly reduced the BCF and TF, resulting in insignificant effect on total Cd uptake of S. nigrum compared with the control. Results of qPCR illustrated that the two strains influenced the detoxification of Cd in S. nigrum by affecting the expression of antioxidant enzyme genes and gene PDR2. Moreover, the differential expression of heavy metal transport genes IRT1 and HMA may lead to the difference of Cd accumulation in S. nigrum. Principal component analysis and Pearson correlation coefficient analysis further verified the positive roles of salicylic acid and indole-3-acetic acid on Cd detoxification of S. nigrum, and the positive correlation relationship between transportation of Cd from underground to shoot, plant biomass and Cd uptake. Altogether, our results demonstrated that these two PGPB have great potential in helping plants detoxify Cd and could provide insights into the mechanism of PGPB-assisted phytoremediation of Cd-contaminated soil.Produced water generated in the recovery of crude oil contains oil and high concentrations of salts, organic matter, and suspended solids and must therefore be treated appropriately prior to disposal. Monolithic ceramic membranes have high oil removal rates and have the advantage of being compact, having a long life, and withstanding chemicals, heat, and high cleaning pressures. Membrane fouling, however, is a significant drawback to membrane filtration. Scrubbing using air bubbles generated by a diffuser is generally used to physically clean membranes. However, monolithic ceramic membranes cannot be scrubbed using air bubbles because their fluid channels are only a few millimeters wide. Membrane washing efficiency was therefore evaluated using fine bubbles smaller than the diameter of the channels. In dead-end filtration, flushing the membrane surface with air-microbubble water or air-ultra-fine bubble (UFB) water after backwashing and air-blowing (conventional cleaning) of the channels was more efficient than conventional cleaning. Flushing with UFB water was not influenced by changes in pH that changed the zeta potential of the UFB. Membrane fouling was suppressed in crossflow filtration by mixing UFB water with feed water. There was no significant change in the diameter of the oil droplets in the feed water before and after UFB mixing. The ZP of the oil droplets peaked at around -20 mV before UFB mixing. However, the peak shifted to around -25 to -29 mV after UFB mixing.Recently, increasing attention has been paid to the inactivation of antibiotic resistant bacteria (ARB) during the electrochemical disinfection. However, no available information could be found on ARB inactivation in water during metal-free electrochemical disinfection. In this study, polyvinylidene fluoride (PVDF)-based carbon catalyst (PPC) was chosen as working electrode. Batch experiments were conducted to investigate key design for ARB inactivation, effects of water matrix and potential risks after the disinfection under the pre-determined conditions. The disinfection with current density at 2.25 mA/cm2 and Air/Water ratio of 101 was optimal with the largest ARB inactivation (5.0 log reduction for 40 min), which was in line with the profile and yield of hydrogen peroxide (H2O2) during the disinfection. Effects of water matrix analysis implied that ARB inactivation efficiencies during the disinfection in acidic solutions were better than the one in alkaline solutions, which could be due to rich CC levels on surface of PPC cathode. After the optimal disinfection, ARB counts increased slightly at the first 2 h and then tended to disappear, and there were no conjugation transfer and little transformation for target antibiotic resistance genes, indicating that potential risks could be blocked after the disinfection for 40 min. Furthermore, intermittent flow was more effective in inactivating ARB compared with continuous flow. These suggested that the application of metal-free electrochemical disinfection with PPC to inactivate ARB in water was feasible and desirable in this study.