Stallingsskinner2059

Rapid and accurate monitoring of cancer cells with high sensitivity is essential for a successful cancer treatment. As high-affinity nucleic acid ligands, aptamers can improve the properties of detection methods by conjugating with intracellular or extracellular cancer biomarkers. Despite the advances in the early detection and treatment of cancer cells, lacking effective early detection tools is one of the causes of a high mortality rate. Aptasensors, which are based on the specificity of aptamer-target recognition, with transduction for analytical purposes have received particular attention due to their high sensitivity and selectivity, simple instrumentation, as well as low production cost. In this review, some selective and sensitive methods were summarized based on advanced nanomaterials towards aptasensing of cancer cells, such as blood, breast, cervical, colon, gastric, liver, and lung cancer cells. This review summarizes advances from 2010 to June 2020 in the development of aptasensors for cancer cell detection. Various aptasensing strategies are assessed according to their potential for reaching relevant limits of sensitivity, specificity, and degrees of multiplexing. Furthermore, we address the remaining challenges and opportunities to integrate aptasensing platforms into point-of-care solutions. Finally, the advantages and limitations of aptamer-based aptasensing strategies were reviewed.A novel dicyanoisophorone (DCI)-based NIR fluorophore employing 2, 4-thiazolidinediones as the modification site was designed for fluorescence imaging. The fluorophore was assessed as a switchable reporter for H2O2 and the probe exhibited lysosomes-targeted, a large turn-on fluorescence signal at 720 nm with a large stokes shift (150 nm) and can be used in biological systems. The ability of the novel fluorophore to emit NIR fluorescence through a "turn-on" activation mechanism makes it a promising fluorophore for in vivo imaging applications. The strategy of introducing the thiazolidinediones with the easy modification site into the fluorophore has a good application prospect to expand the application of the NIR fluorophore.Single nucleotide polymorphism (SNP) analysis based on allele-specific polymerase chain reaction (AS-PCR) is a relatively effective and economical method compared with other genotyping technologies such as DNA sequencing, DNA hybridization and isothermal amplification strategies. Tariquidar chemical structure But AS-PCR is limited by its labor-intensive optimization of reaction parameters and time-consuming result assessment. In this study, we put forward a novel idea of data processing to address this problem. SNP analysis was accomplished by AS-PCR with endpoint electrochemical detection. For each sample, two separate reactions were run simultaneously with two sets of allele-specific primers (wild-type primers for W system and mutant primers for M system). We measured their redox current signals on screen-printed electrodes once AS-PCR finished and calculated the difference value of current signals between two systems to determine the genotyping result. Based on the difference value of fluorescent signals, real-time fluorescent PCR was used to study reaction parameters in AS-PCR. With screened parameters, we obtained the genotyping results within 50 min. 36 hair-root samples from volunteers were analyzed by our method and their genotypes of ALDH2 gene (encoding aldehyde dehydrogenase 2) were totally identical with data from commercialized sequencing. Our work first employed difference value between two reaction systems to differentiate allele and provided a novel idea of data processing in AS-PCR method. It is able to promote the quick analysis of SNP in the fields of health monitor, disease precaution, and personalized diagnosis and treatment.A simple and fast method for copper ions (Cu2+) and silver ions (Ag+) detection was established with cadmium telluride quantum dots (CdTe QDs) as fluorescent probes. In the presence of Cu2+ or Ag+, the fluorescence intensity of TGA-CdTe QD can be significantly quenched, which fitted a linear relationship between the fluorescence quenching degree (F0-F)/F0 and the concentration of metal ions. In this work, the lowest detected concentration for Cu2+ and Ag+ was 35.0 nM and 25.3 nM, respectively. In addition, the differentiation of Cu2+ and Ag+ at different concentrations was realized with the principal component analysis (PCA). Furthermore, Cu2+ was successfully detected in body fluids. This method provides a good potential for copper ions and silver ions detection with simplicity, rapidity, and excellent selectivity.Circulating tumor DNA (ctDNA) is a promising biomarker for tumor genotyping and therapy monitoring. Herein, we developed a digital PCR chip with embedded microwell and bidirectional partition network for highly sensitive ctDNA analysis. The embedded microwell contributes to increasing microreaction density (up to 7000 microwells/cm2) and reducing evaporation during amplification. The bidirectional partition network can achieve fast and random distribution of targets, ensuring the precise quantification of nucleic acid. We used plasmids, artificial samples and 32 clinical blood samples from non-small cell lung cancer patients to test the performance of this platform. The results demonstrated that our chip has not only comparable quantification performance to commercial counterpart but also the ability to detect EGFR mutations with as low as 0.01% mutation rate and 20 alter molecules in 27 ng genomic DNA. The identification of EGFR mutations in plasma using developed chip exhibited 85.71% sensitivity and 94.44% specificity for L858R mutation and 100% sensitivity and 86.96% specificity for T790 M mutation. Moreover, the monitoring of mutant allele in plasma was accomplished in this work. In conclusion, the developed chip has a potential in lung tumor genotyping and therapy monitoring for precision medicine, even other tumors.A novel on-site preparation strategy for the determination of trace aromatic amines (AAs) in environmental waters was developed in the present study. To extract AAs effectively, 4-vinylbenzoic acid was copolymerized with ethylene dimethacrylate (ED)/divinylbenzene (DVB) in a pipette tip to obtain a new monolith-based adsorbent (MBA). The MBAs were employed as the extraction phases of home-made multichannel in-tip microextraction apparatus (ITMA) which was used to perform field sample preparation of AAs in different water samples followed by HPLC/DAD analysis. Due to the abundant functional groups, the prepared MBA displayed satisfying extraction performance for studied AAs. Under the optimized conditions, limits of detection varied from 2.1 to 26 ng/L with good coefficients of determination and precision. The recoveries for real water samples with different fortified concentrations were in the range of 78.1-119%, and the RSD values varied from 0.85 to 11%. In addition, the results achieved with the introduced method were well comparable to that obtained with conventional laboratory sample pretreatment process.