Slaterfranco2138

PURPOSE Angiogenesis is considered as a major progenitor in the progression of obesity. The current manuscript enumerates the extrinsic role of angiogenesis in obesity. RESULT High caloric diet and lack of physical exercise are the most common causes of obesity and related metabolic conditions. A grossly elevated levels of fat in adipose tissue escalate certain complications which further worsen the state of obesity. Enlargement of white adipose tissue (WAT), deposition of fat mass, proliferation of endothelial cells, production of inflammatory cytokines induces the formation of denovo capillaries from parent microvasculature. Also, several intracellular signaling pathways precipitate obesity. Though, angiostatic molecules (endostatin, angiostatin and TNP-470) have been designed to combat obesity and associated complications. CONCLUSION Adipose tissue trigger growth of blood capillaries, and in turn adipose tissue endothelial cells promote pre-adipocyte proliferation. Modulation of angiogenesis and treatment with angiostatic substances may have the potential to impair the progression of obesity. In developing countries, organic waste is commonly dumped in untreated landfills. Brazil has around 3,000 landfills distributed in 1,600 cities. The Brazilian government instituted the National Solid Waste Policy (PNRS), which set the goal of ending the dumping grounds in 2014. However, it was not accomplished. Municipal governments do not have the resources to fund the existing treatment technologies. Thus, there is an urgent need to propose low-cost alternatives to solve this problem. This work proposes a low-cost Product-Service System (PSS) solution that enables the proper treatment of organic waste using the current municipal budget, based on the renting of household biodigesters. click here It was outlined from a model built on various proposals for the PSS design, published between 2011 and 2018. A feasibility simulation of the proposal conducted for the scenario of the Brazilian city of Porto Amazonas has demonstrated that, by adopting the proposal, the administration could comply with the PNRS and save part of its actual budget. It also showed 75% of probability of financial viability for the PSS provider, in a scenario of reduction of up to 20% in the current municipal costs for organic waste collection and transportation, and up to 40% off in cooking gas costs for the equipment-hosting households. The proposal is a potential solution for the Brazilian municipalities with more favorable scenarios to comply with the PNRS, as well as other countries' municipalities with budget constraints to eliminate their dumps. There is an increasing need for landfill leachate pretreatment prior to discharge to wastewater treatment plants due to increasingly stringent sewer discharge limits. Lab-scale tests have shown that the anaerobic biological processes can effectively remove chemical oxygen demand and dissolved organic carbon from landfill leachate. Our work expands the knowledge in anaerobic leachate pretreatment by systematically studying the conversion of carbon and nitrogen species, particularly their recalcitrant fractions in a submerged anaerobic biofilm reactor using real-world leachate from a typical young municipal solid waste landfill. After reaching steady state, the reactor removed 41.7% of the fulvic acids (i.e., 1290 mg C/L). While compounds with a low degree of oxidation (OC  0.07). The submerged anaerobic biofilm reactor may be an efficient leachate pretreatment method if rDON removal is needed. Freeze-drying technology has been widely considered for decades as a suitable technique to preserve microorganisms. However, protective agents must be added prior to freeze drying to improve the survival and storage stability of the bacteria. The objective of our study was to evaluate the effect of a new protectant medium containing sucrose (10 %), trehalose (10 %), skimmed milk (10 %) and antioxidants on the viability of gut bacteria under different storage conditions. Two strains were tested, Escherichia coli and Akkermansia muciniphila, as examples of facultative aerobic and anaerobic bacteria, respectively. We studied the cell viability and bacterial morphology in 5 fecal samples in the presence and absence of this protectant medium using plating technique, flow cytometry and scanning electron microscopy. The results of bacterial viability assessed by plating method showed that the protectant medium yielded higher survival rates for both strains whatever the storage conditions (85-93 %) compared to normal saline solution (0.36-37.50 %). It also showed its effectiveness on fecal samples, where bacterial viability after freeze-drying was 89.47 ± 7.63 % and 84.01 ± 7.44 %, as evidenced by flow cytometry analysis and plating method. However unprotected samples showed the lowest cell viability at 19.01 ± 12.88 % and 13.23 ± 9.56 %, as measured by flow cytometry and plating method. In addition, bacterial size and shape were conserved in the protectant medium. In contrast, storage without protectant medium severely damaged bacterial morphology. In conclusion, our study is the first to use morphological features as well as culture-dependant and culture-independent tests to evaluate the effectiveness of a new protectant medium. Harmful cyanobacterial blooms are increasingly common in aquatic environments. This can lead to higher concentrations of cyanotoxins, such as microcystins (MCs), posing a great risk to diverse organisms, including humans. MCs are among the most commonly reported cyanotoxins in freshwater environments worldwide, where they may have different fates. MCs can adsorb to suspended particles into the water column and deposit onto the sediment where they can be affected by physical factors (e.g. winds in shallow lakes causing sediment resuspension) or biological factors (e.g. biodegradation). Here we focused on the conditions of a coastal shallow lagoon contaminated by MCs aiming to estimate the return of pre-existing MCs from the sediment to the water column, to evaluate the adsorption of dissolved MC-LR to the sediment and to verify the occurrence of biodegradation. In experiments with sediment, desorption and adsorption were tested under the influence of temperature, pH and aeration, reproducing conditions observed in the lagoon.