Sherwoodhuff6519

RESULTS All of the formula showed a homogen, pH around 6, peeling time less than 20 minutes, no irritation and stable for 12 weeks storage at room temperature. The treatment with PGMRB demonstrated an improvement on the moisture level, pore size, evenness and the number of black spots with F3 containing 5% extract as the best formula. CONCLUSION The ethanol extract of red bean can be formulated become a peel-off gel mask and having the anti-aging effect. Copyright © 2019 Bayu Eko Prasetyo, Dwi Rafika, Lia Laila, Fat Aminah.BACKGROUND Cancer is one of the causes of morbidity and mortality worldwide. Breast cancer is one of the most common types of cancer in Indonesia. Failures that often occur in the treatment of cancer primarily through chemotherapy, synthetic drugs that have side effects include anemia, alopecia, cardiotoxic and hepatotoxic due to low anti-cancer selectivity and unclear carcinogenesis process. Cyperus rotundus L. rhizome is one of the medicinal plants that potential enough to be developed as an anticancer agent. AIM The aim of this study was to anticancer activity n-hexane fraction Cyperus rotundus L. rhizomes to breast cancer MCF-7 cell line in vitro. METHODS Cyperus rutundus L. rhizomes powder was extracted ethanol by percolation then fractionated with n-hexane. Phytochemical screening was then carried out. The cytotoxic activity of the n-hexane fraction was determined by observing this extract on MCF-7 cells using the (3- (4,5-dimethylimidazole-2-il) -2,5-diphenyl tetrazolium bromide) (MTT). Selectivity index (IS) of normal cells (Vero cells). Cell cycle and apoptosis induction were analyzed by flow cytometry. RESULTS The result showed that the fraction n-hexane Cyperus rutundus L. rhizome has anticancer activity against breast cancer MCF-7 cells with accumulation cell cycle in the G0-G1 phase and through induction of apoptosis. CONCLUSION The n-hexane fraction Cyperus rotundus L. rhizome has potent anticancer activity. Copyright © 2019 Delisma Simorangkir, Masfria Masfria, Urip Harahap, Denny Satria.BACKGROUND Halitosis is one of the unpleasant conditions which could alter the self-confidence and cause serious illness. One of the treatments to overcome halitosis is by using mouthwash. Plectranthus amboinicus (Lour.) Spreng is one of the plants that is known to have antibacterial activity which can be used to overcome halitosis caused bacteria. AIM To formulate the Plectranthus amboinicus (Lour.) Spreng leaves ethanolic extract as herbal mouthwash and to evaluate the antibacterial activity against Streptococcus mutans and Staphylococcus aureus bacteria. METHODS The methods of the study included the characterization and screening of Plectranthus amboinicus (Lour.) Spreng leaves dried powder; the extraction process by maceration using 96% ethanol; the antibacterial activity test of Plectranthus amboinicus (Lour.) Spreng leaves ethanolic extract and the formulation and evaluation of mouthwash dosage form with various concentrations of Plectranthus amboinicus (Lour.) Spreng leaves ethanolic extract. RESULTS The characterization results showed that the Plectranthus amboinicus (Lour.) Spreng leaves powder contained 7.92% of water, 29.26% of water-soluble substance, 13.32% of ethanol soluble substance, 0.82% of total ash and 0.66% of acid insoluble ash. The screening examination gave glycoside, saponin, flavonoid, tannin and steroid/triterpenoid positive results. The antibacterial activity test of the Plectranthus amboinicus (Lour.) Spreng mouthwash showed that the dosage form with 2% of Plectranthus amboinicus (Lour.) Spreng extract successfully inhibited the growth of bacteria with 12.00 and 11.25 mm of inhibition diameter for both Staphylococcus aureus and Streptococcus mutans, respectively. CONCLUSION The Plectranthus amboinicus (Lour.) Spreng leaves ethanolic extract can be formulated in mouthwash dosage form and has antibacterial activity against Staphylococcus aureus and Streptococcus mutans bacteria. Copyright © 2019 Nazliniwaty Nazliniwaty, Lia Laila.BACKGROUND Agarwood tree (Aquilaria malaccensis Lamk) is a tree from the Thymeleaceae tribe that has a high selling value. The part that is often used is the sapwood of the agarwood tree, used as a base for perfumes and traditional medicines. Agarwood farmers in Langkat have used their leaves as tea drinks, brewing aloes leaves. Before being widely produced by the community, it is necessary to test the safety of agarwood leaf tea products through skin sensitization test. AIM To find out whether steeping aloes leaves irritate the skin or not. METHODS The test method using test animals namely albino rabbits as many as 15 tails, consisting of 4 test groups 1 group there were 3 test animals and 1 control group, induced with Freund's Complete Adjuvant (FCA) by intradermal and topical injection with a test position of 1.3%, 2.6%, 3.9% and 5.2% to form an immune response, then a Challenge test. The level and degree of skin reaction were assessed based on the Magnusson and Kligman scales. RESULTS From the observation for 72 hours there was no change in the skin of the test animals after exposure to the test doses, indicating that the agarwood leaf tea is safe to consume if the tea is on the skin, the skin will not experience irritation. CONCLUSION Testing on albino rabbits with four different doses did not show an irritating effect on the skin. Observation also shows that there is not a single bit that can affect intradermal to challenge testing so it was safe for consumption. Copyright © 2019 Surjanto Surjanto, Ridwanti Batubara, Dedy Syahputera Rangkuti.AIM The study aimed to isolate and identification secondary metabolite from pericarp Garcinia mangostana Linn. METHODS The first step of this research was maceration of sample using alcohol 70% solvent. The separation and purification of compounds using Vacuum Liquid Chromatography (VLC), Radial Chromatography (RC). The purity of isolate was analyzed by thin layer chromatography (TLC) and melting point. Compounds identified using spectroscopi IR, NMR-1D (1H, 13C-NMR and DEPT) and 2-D NMR (HMQC and HMBC). RESULTS The compound has melting point at 165-167°C. The result showed isolate was gartanin. CONCLUSION The secondary metabolite found in pericarp Garcinia mangostana Linn. Selleckchem CHIR-98014 is gartanin. Copyright © 2019 RA Oetari, Hasriyani Hasriyani, Adi Prayitno, Sahidin Sahidin.