Peterssonvilhelmsen0293

The observed stability of recombinant xylanase at higher temperature and in the presence of organic solvent (ethanol, butanol) suggested possible application of this enzyme in biofuel and other industrial applications.The formation of chitosan dimer and its interaction with urea and creatinine have been investigated at the density functional theory (DFT) level (B3LYP-D3/6-31++G**) to study the transport phenomena in hemodialysis membrane. The interaction energy of chitosan-creatinine and chitosan-urea complexes are in range -4 kcal/mol less then interaction energy less then -20 kcal/mol which were classified in medium hydrogen bond interaction. The chemical reactivity parameter proved that creatinine was more electrophilic and easier to bind chitosan than urea. The energy gap of HOMO-LUMO of chitosan-creatinine complex was lower than chitosan-urea complex that indicating chitosan-creatinine complex was more reactive and easier to transport electron than chitosan-urea complex. Moreover, the natural bond orbital (NBO) analysis showed a high contribution of hydrogen bond between chitosan-creatinine and chitosan-urea. The chitosan-creatinine interaction has a stronger hydrogen bond than chitosan-urea through the interaction O18-H34....N56 with stabilizing energy = -13 kcal/mol. The quantum theory atom in molecule (QTAIM) also supported NBO data. All data presented that creatinine can make hydrogen bond interaction stronger with chitosan than urea, that indicated creatinine easier to transport in the chitosan membrane than urea during hemodialysis process.Recently, photo-degradation process under ultraviolet-light irradiation is being used as a substantial treatment method for the removal of environmental pollution. In this study, a silver phosphate-ilmenite (Ag3PO4-FeTiO3) hetero structure supported on glycol chitosan catalyst was completely prepared, also, and its structural, and optical properties were characterized. Meantime, scanning electron microscopy, X-ray diffraction, X-ray photoelectron, and UV-vis spectra were applied. The Ag3PO4-FeTiO3/glycol chitosan catalyst was used to degrade metronidazole under visible-light irradiation. The degradation rate of metronidazole in 25 min by Ag3PO4-FeTiO3/glycol chitosan nanocomposites was found to be 99.2% under UV light irradiation, which was higher than that by Ag3PO4-FeTiO3 (72.24%) and FeTiO3 (35.5%), respectively. The active species trapping test of Ag3PO4-FeTiO3/glycol chitosan indicated that ·OH and ·O2- participated during the reaction. The diffusion method was evaluated to appraise the bactericidal activity of the synthesized nanomaterials when tested against both Staphylococcus aureus and Escherichia coli bacteria, with or without LED-light irradiation. The antibacterial tests show higher inhibition zones under light illumination as compared to dark conditions. The antifungal properties of the prepared nanomaterials were analyzed by fungi (Aspergillus niger, and Fusarium solani) using disc diffusion analysis. It was confirmed that the prepared nanomaterials have the best antifungal agent as compared to the standard antibiotics. When the Ag3PO4-FeTiO3/glycol chitosan was used, the amount of inhibition zone was enhanced.The main purpose of this study was to explore the ability of a novel silica/polysaccharide polymer-based formulation, namely, chitosan/silica nanocomposites (CSNs), to directly affect Botrytis cinerea in vitro and in inoculated berries, and indirectly to induce natural host resistance via enzymatic and nonenzymatic antioxidants against gray mold of table grapes. The results indicated a positive correlation in in vitro tests in terms of radial growth, spore germination and germ tube elongation, where those parameters were completely inhibited by CSN at 1%. SEM and TEM investigations showed that morphological and internal structural damage was observed in B. cinerea-hyphae/spores treated with CSN. Additionally, most of the treated spores were affected, and cellular vacuolization and cytoplasmic disorganization were observed. The results revealed that CSN reduced gray mold incidence and severity on inoculated berries directly and indirectly. In direct activity, CSN (1%) reduced mold incidence and severity by 100% compared to the control. In indirect activity, mold incidence and severity was reduced by 51% and 64%, respectively. CSN significantly increased superoxide dismutase, ascorbate peroxidase, peroxidase, total phenol and flavonoid at 48 h post-treatment by 1.2-, 1.6-, 1.3-, 1.3- and 1.6-fold, respectively, in grape-treated tissues. It could be concluded that CSN, as a promising alternative control method against gray mold of table grapes, can directly affect the pathogen and indirectly enhance the natural host resistance of the antioxidant system.The effects of ball-milling on the pasting properties of waxy maize starch (WMS) and waxy rice starch (WRS) were investigated from a multiscale structural view. The results confirmed that ball-milling significantly destroyed the structures of the two waxy starches (especially WMS). Specifically, ball-milling led to obvious grooves on the surface of starch granules, a decrease in crystallinity and the degree of short-range order, and a reduction in double-helix components. Eribulin Meanwhile, small-angle X-ray scattering results indicated that the semicrystalline lamellae of starch were disrupted after ball-milling. Ball-milling decreased the pasting temperatures. Furthermore, ball-milled starches exhibited lower peak and breakdown viscosity and weakened tendency to retrogradation. These results implied that ball-milling induced structural changes in starch that significantly affected its pasting properties. Hence, ball-milled starch may serve as food ingredients with low pasting temperature and paste viscosity as well as high paste stability under heating/cooling and shearing.A novel nanoparticle (NP) delivery carrier for curcumin based on electrostatic 6-deoxy-6-arginine modified chitosan (DAC) assembled by γ-poly-glutamic acid (γ-PGA) was prepared. The NP structure was evaluated using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Interactions between DAC and γ-PGA were characterized using Fourier transform infrared spectroscopy (FT-IR). The sustained release kinetics of curcumin-loaded NPs was investigated in simulated gastrointestinal fluids. After exposed to heating, pH, and NaCl aqueous solution, the stabilities of both normal and curcumin-loaded NPs were determined. The results showed that NPs achieved a high encapsulation efficiency (79.5%) and loading capacity (11.31%) for curcumin. The curcumin-loaded NPs displayed a sustained release profile under simulated gastrointestinal conditions. Under certain pH (3-9), salt (0-100 mM), and temperature (30 - 60 °C) conditions, the vehicles of curcumin showed better stability. This demonstrates that NPs can be used as stable carriers for curcumin.The creation of multi-functional bio-hydrogels with tunable properties that meet in vivo demands is significant but remains challenging. Inspired by host-guest chemistry, a novel multi-functional gelatin-based bio-hydrogel with tunable antibacterial and hemorheological properties (TAH-GEL) is synthesized via an in situ one-pot strategy. TAH-GEL not only exhibits excellent mechanical properties but also shows promising self-healing and bio-compatibility features. For the first time, this biomaterial presents controllable antibacterial and hemorheological properties by controlling the TAH-GEL polypseudorotaxane motif. The resulting bio-hydrogel is easy to prepare and delivers superior performance, making it a powerful tool for bio-applications, such as hemostatic materials.In this study, antibacterial nanofiber films were prepared by electrospinning gelatin, chitosan, and 3-phenyllactic acid (PLA). The addition of PLA improved the microstructures of the nanofibers, and the nanofiber films (GCP-1 and GCP-2) had uniform and continuous structures with a diameter range of 40--70 nm when the PLA concentrations in the polymers were 1% and 2%. Under acidic conditions, chitosan and PLA interacted and formed hydrogen bonds, which decreased the crystallinity of the nanofiber films. The GCP-2 nanofiber film had the best thermal stability, water stability, and water vapor permeability. link2 Compared with the control GCP-0 film, the four nanofiber films with PLA (GCP-1, GCP-2, GCP-3, and GCP-4) had more effective antibacterial effects, and GCP-2 film reduced approximately 4 log CFU/mL of Salmonella enterica Enteritidis and Staphylococcus aureus in 30 min. Results suggested that the GCP-2 nanofiber film mat can be used as an active food packaging.In this research, a novel polysaccharide (PCP) was extracted from Pleurotus citrinopileatus and purified by Sephadex G-150 gel column, and its antitumor activity was investigated using the model H22 tumor-bearing mice. PCP was found to be composed of arabinose, galactose, glucose, xylose, mannose and glucuronic acid in a proportion of 0.66 14.59 10.77 1 0.69 0.23 with average molecular weight of 7.30 × 105 Da. Further analysis suggested that PCP was a pyranose with α-type and β-type glycosidic residues. The antitumor assays in vivo indicated that PCP could effectively suppress H22 solid tumor growth, protect immune organs and improve inflammation and anemia. Besides, Annexin V-FITC/PI double staining and JC-1 staining demonstrated that PCP could induce apoptosis of H22 hepatoma cells. The PI staining assay revealed that PCP induced H22 hepatoma cells apoptosis by arresting cell cycle in S phase. These results suggest that the polysaccharide from Pleurotus citrinopileatus possesses potential value in the treatment of liver cancer.SARS-CoV-2is the causative agent for the ongoing COVID19 pandemic, and this virus belongs to the Coronaviridae family. The nsp14 protein of SARS-CoV-2 houses a 3' to 5' exoribonuclease activity responsible for removing mismatches that arise during genome duplication. A homology model of nsp10-nsp14 complex was used to carry out in silico screening to identify molecules among natural products, or FDA approved drugs that can potentially inhibit the activity of nsp14. This exercise showed that ritonavir might bind to the exoribonuclease active site of the nsp14 protein. A model of the SARS-CoV-2-nsp10-nsp14 complex bound to substrate RNA showed that the ritonavir binding site overlaps with that of the 3' nucleotide of substrate RNA. A comparison of the calculated energies of binding for RNA and ritonavir suggested that the drug may bind to the active site of nsp14 with significant affinity. link3 It is, therefore, possible that ritonavir may prevent association with substrate RNA and thus inhibit the exoribonuclease activity of nsp14. Overall, our computational studies suggest that ritonavir may serve as an effective inhibitor of the nsp14 protein. nsp14 is known to attenuate the inhibitory effect of drugs that function through premature termination of viral genome replication. Hence, ritonavir may potentiate the therapeutic properties of drugs such as remdesivir, favipiravir and ribavirin.A novel thermostable xylanase gene from Chaetomium sp. CQ31 was cloned and codon-optimized (CsXynBop). The deduced protein sequence of the gene shared the highest similarity of 75% with the glycoside hydrolase (GH) family 10 xylanase from Achaetomium sp. Xz-8. CsXynBop was over-expressed in Pichia pastoris GS115 by high-cell density fermentation, with the highest xylanase yield of 10,017 U/mL. The recombinant xylanase (CsXynBop) was purified to homogeneity and biochemically characterized. CsXynBop was optimally active at pH 6.5 and 85 °C, respectively, and stable over a broad pH range of 5.0-9.5 and up to 60 °C. The enzyme exhibited strict substrate specificity towards oat-spelt xylan (2, 489 U/mg), beechwood xylan (1522 U/mg), birchwood xylan (1067 U/mg), and showed relatively high activity towards arabinoxylan (1208 U/mg), but exhibited no activity on other tested polysaccharides. CsXynBop hydrolyzed different xylans to yield mainly xylooligosaccharides (XOSs) with degree of polymerization (DP) 2-5. The application of CsXynBop (200 U/g malt) in malt mashing substantially decreased the filtration time and viscosity of malt by 42.